CD41a Mouse mAb

COA

级别和纯度:

无载体

已验证

ExactAb™

见COA

应用:

Flow Cytometry

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG1
克隆号: HIP8
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab094804-10μg	10μg	现货
Ab094804-50μg	50μg	现货
Ab094804-100μg	100μg	期货
Ab094804-1mg	1mg	期货

查看相关系列

点击查看靶蛋白信息: ITGA2B

Accession#:P08514 (25) CD41 (7) Gene ID:3674 (25) ITGA2B (25) 流式细胞术 (1163)

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基本信息

产品名称	CD41a Mouse mAb
别名	血小板膜糖蛋白Ⅱb(CD41)单克隆抗体 \| Anti-CD41a抗体 \| CD41a抗体 \| CD41a单抗 \| CD41a 小鼠单克隆抗体 \| CD41a 小鼠单抗
英文别名	CD41 antigen \| CD41 \| CD41BHPA3 \| GP2B \| GP2Bintegrin alpha-IIb \| GPalpha IIb \| GPIIb \| GTA \| HPA3 \| Integrin alpha 2b \| integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41) \| integrin, alpha 2b (platelet glycoprotein IIb of IIb
规格或纯度	无载体, ExactAb™, 已验证, 见COA
宿主种属	小鼠(Mouse)
特异性	CD41a
种属反应性	人(Human)
免疫原	Purified platelet membrane glycoproteins
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	HIP8
Format	Whole IgG
亚型	Mouse IgG1
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 0.1% Sodium azide, pH 7.2
防腐剂	0.1% Sodium azide
浓度	见COA
储存温度	2-8°C储存
运输条件	冰袋运输
稳定性与储存	4°C 下运输。4°C 长期保存（36 个月）。收货后建议分装。
分子类型	抗体

关联靶点（人）

ITGA2B Tclin 整合素α-IIb(Integrin alpha-IIb) (0 活性数据)

点击查看靶蛋白信息： ITGA2B

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Flow Cytometry: CD41a Mouse mAb (Ab094804)

CD41a Mouse mAb (Ab094804) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood monocytes labelling CD41a with CD41a Mouse mAb (Ab094804) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD41a Mouse mAb (Ab094804)

CD41a Mouse mAb (Ab094804) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood monocytes labelling CD41a (red) with CD41a Mouse mAb (Ab094804) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD41a Mouse mAb (Ab094804)

CD41a Mouse mAb (Ab094804) - Flow Cytometry
Flow Cytometry analysis of HEL cells labelling CD41a (red) with CD41a Mouse mAb (Ab094804) at 0.05 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	< 0.5µg/100w cells

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找到2个结果

批号(Lot Number)	证书类型	货号
ZJ24F0404000	分析证书	Ab094804
ZJ24F0403999	分析证书	Ab094804

技术文档和文章

利用甲醇通透法进行流式细胞术

Categories: 实验方案（Protocols）

探索主题:

流式细胞术流式抗体流式实验甲醇通透细胞分选细胞分型免疫研究细胞内抗原检测

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

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引用文献

1. Miller WH, Bondinell WE, Cousins RD, Erhard KF, Jakas DR, Keenan RM, Ku TW, Newlander KA, Ross ST, Haltiwanger RC, Bradbeer J, Drake FH, Gowen M, Hoffman SJ, Hwang SM, James IE, Lark MW, Lechowska B, Rieman DJ, Stroup GB, Vasko-Moser JA, Zembryki DL, Azzarano LM, Adams PC, Huffman WF.. (1999) Orally bioavailable nonpeptide vitronectin receptor antagonists with efficacy in an osteoporosis model.. Bioorg Med Chem Lett, 9 (13): (1807-1812). [PMID:10406646] [10.1016/s0960-894x(99)00283-8]
2. Weller T, Alig L, Beresini M, Blackburn B, Bunting S, Hadváry P, Müller MH, Knopp D, Levet-Trafit B, Lipari MT, Modi NB, Müller M, Refino CJ, Schmitt M, Schönholzer P, Weiss S, Steiner B.. (1996) Orally active fibrinogen receptor antagonists. 2. Amidoximes as prodrugs of amidines.. J Med Chem, 39 (16): (3139-3147). [PMID:8759635] [10.1021/jm9509298]
3. Badorc A, Bordes MF, de Cointet P, Savi P, Bernat A, Lalé A, Petitou M, Maffrand JP, Herbert JM.. (1997) New orally active non-peptide fibrinogen receptor (GpIIb-IIIa) antagonists: identification of ethyl 3-[N-[4-[4-[amino[(ethoxycarbonyl) imino]methyl]phenyl]-1,3-thiazol-2-yl]-N-[1-[(ethoxycarbonyl)methyl]pip erid -4-yl]amino]propionate (SR 121787) as a potent and long-acting antithrombotic agent.. J Med Chem, 40 (21): (3393-3401). [PMID:9341914] [10.1021/jm970240y]
4. Okumura K, Shimazaki T, Aoki Y, Yamashita H, Tanaka E, Banba S, Yazawa K, Kibayashi K, Banno H.. (1998) New platelet fibrinogen receptor glycoprotein IIb-IIIa antagonists: orally active series of N-alkylated amidines with a 6,6-bicyclic template.. J Med Chem, 41 (21): (4036-4052). [PMID:9767641] [10.1021/jm9801859]
5. Hoekstra WJ, Maryanoff BE, Damiano BP, Andrade-Gordon P, Cohen JH, Costanzo MJ, Haertlein BJ, Hecker LR, Hulshizer BL, Kauffman JA, Keane P, McComsey DF, Mitchell JA, Scott L, Shah RD, Yabut SC.. (1999) Potent, orally active GPIIb/IIIa antagonists containing a nipecotic acid subunit. Structure-activity studies leading to the discovery of RWJ-53308.. J Med Chem, 42 (25): (5254-5265). [PMID:10602710] [10.1021/jm990418b]
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8. Calvete JJ, Alvarez MV, Rivas G, Hew CL, Henschen A, González-Rodríguez J. Interchain and intrachain disulphide bonds in human platelet glycoprotein IIb. Localization of the epitopes for several monoclonal antibodies.. Biochem J, [PMID:2476117]
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18. Uzan, G G and 9 more authors.. (1988) cDNA clones for human platelet GPIIb corresponding to mRNA from megakaryocytes and HEL cells. Evidence for an extensive homology to other Arg-Gly-Asp adhesion receptors.. European journal of biochemistry, (15): [PMID:3422188]
19. Bray, P F PF and 8 more authors.. (1987) Platelet glycoprotein IIb. Chromosomal localization and tissue expression.. The Journal of clinical investigation, [PMID:3479442]
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21. Hiraiwa, A A and 5 more authors.. (1987) Purification and partial amino acid sequence of human platelet membrane glycoproteins IIb and IIIa.. Blood, [PMID:3801670]
22. Wilcox, D A DA, Wautier, J L JL, Pidard, D D and Newman, P J PJ.. (1994) A single amino acid substitution flanking the fourth calcium binding domain of alpha IIb prevents maturation of the alpha IIb beta 3 integrin complex.. The Journal of biological chemistry, (11): [PMID:7508443]
23. Calvete, J J JJ and Muñiz-Diaz, E E.. (1993) Localization of an O-glycosylation site in the alpha-subunit of the human platelet integrin GPIIb/IIIa involved in Baka (HPA-3a) alloantigen expression.. FEBS letters, (9): [PMID:7688323]
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28. Basani, R B RB and 5 more authors.. (1996) Glanzmann thrombasthenia due to a two amino acid deletion in the fourth calcium-binding domain of alpha IIb: demonstration of the importance of calcium-binding domains in the conformation of alpha IIb beta 3.. Blood, (1): [PMID:8704171]
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30. French, D L DL and Coller, B S BS.. (1997) Hematologically important mutations: Glanzmann thrombasthenia.. Blood cells, molecules & diseases, [PMID:9215749]
31. Grimaldi, C M CM and 5 more authors.. (1998) Glycoprotein IIb Leu214Pro mutation produces glanzmann thrombasthenia with both quantitative and qualitative abnormalities in GPIIb/IIIa.. Blood, (1): [PMID:9473221]
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33. Ruan, J J and 6 more authors.. (1998) Double heterozygosity of the GPIIb gene in a Swiss patient with Glanzmann's thrombasthenia.. British journal of haematology, [PMID:9734640]
34. Tadokoro, S S and 9 more authors.. (1998) A Gln747-->Pro substitution in the IIb subunit is responsible for a moderate IIbbeta3 deficiency in Glanzmann thrombasthenia.. Blood, (15): [PMID:9763559]
35. Trikha, M M, Cai, Y Y, Grignon, D D and Honn, K V KV.. (1998) Identification of a novel truncated alphaIIb integrin.. Cancer research, (1): [PMID:9809974]
36. González-Manchón, C C and 7 more authors.. (1999) Molecular genetic analysis of a compound heterozygote for the glycoprotein (GP) IIb gene associated with Glanzmann's thrombasthenia: disruption of the 674-687 disulfide bridge in GPIIb prevents surface exposure of GPIIb-IIIa complexes.. Blood, (1): [PMID:9920835]
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44. Tanaka, Shigenori S and 7 more authors.. (2002) A Leu55 to Pro substitution in the integrin alphaIIb is responsible for a case of Glanzmann's thrombasthenia.. British journal of haematology, [PMID:12181054]
45. Mitchell, W Beau WB and 6 more authors.. (2003) Two novel mutations in the alpha IIb calcium-binding domains identify hydrophobic regions essential for alpha IIbbeta 3 biogenesis.. Blood, (15): [PMID:12424194]
46. Kiyoi, Teruo T and 9 more authors.. (2003) A naturally occurring Tyr143His alpha IIb mutation abolishes alpha IIb beta 3 function for soluble ligands but retains its ability for mediating cell adhesion and clot retraction: comparison with other mutations causing ligand-binding defects.. Blood, (1): [PMID:12506038]
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溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

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g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

CD41a Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器