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CD3D Mouse mAb

    应用:
  • IHC
  • WB
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human), 猪(Pig)
  • 亚型: Mouse IgG2b
  • 克隆号: C6
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab094722-10μl
10μl 现货 Stock Image
Ab094722-50μl
50μL 现货 Stock Image
Ab094722-100μl
100μL 期货 Stock Image
Ab094722-1ml
1ml 期货 Stock Image

基本信息

产品名称 CD3D Mouse mAb
别名 CD3 delta单克隆抗体 | CD3D小鼠单克隆抗体 | CD3D小鼠单抗
英文别名 CD3 antigen delta subunit antibody | CD3 delta antibody | CD3-delta antibody | CD3d antibody | CD3d antigen delta polypeptide (TiT3 complex) antibody | CD3d molecule antibody | CD3d molecule delta (CD3-TCR complex) antibody | CD3D_HUMAN antibody | IMD19 a
规格或纯度 无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属 小鼠(Mouse)
特异性 CD3D
种属反应性 人(Human),猪(Pig)
免疫原 Recombinant CD3D expressed in E.coli (AA 18-113).
阳性对照 WB: Jurkat and MOLT-4 cell lysates. IHC: Human small intestine and spleen tissues.
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 C6
Format Whole IgG
亚型 Mouse IgG2b
轻链亚型 kappa
SDS-PAGE 150 kDa
纯化方法 Protein A purified + Protein G purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂 0.05% Proclin 300
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

CD3D Tclin T 细胞表面糖蛋白 CD3 δ 链(T-cell surface glycoprotein CD3 delta chain) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

图片

CD3D Mouse mAb (Ab094722) - Western Blot
All lanes: CD3D Mouse mAb (Ab094722) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 19 kDa
Observed band size: 23 kDa
Exposure time: 20.0 s

CD3D Mouse mAb (Ab094722) - IHC
DAB staining on IHC
Sample: Human Small intestine Tissue
Primary Ab: 10 µg/mL CD3D Mouse mAb (Ab094722)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

CD3D Mouse mAb (Ab094722) - IHC
DAB staining on IHC
Sample: Human Spleen Tissue
Primary Ab: 10 µg/mL CD3D Mouse mAb (Ab094722)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

应用

应用名称 稀释比例
IHC

5-20 µg/mL

WB

0.01-2 µg/mL

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ24F0404441 分析证书 Ab094722
ZJ24F0404440 分析证书 Ab094722
ZJ24F0404439 分析证书 Ab094722

引用文献

1. de Saint Basile G, Geissmann F, Flori E, Uring-Lambert B, Soudais C, Cavazzana-Calvo M, Durandy A, Jabado N, Fischer A, Le Deist F.  (2004)  Severe combined immunodeficiency caused by deficiency in either the delta or the epsilon subunit of CD3..  J Clin Invest,  114  (10): (1512-7).  [PMID:15546002]
2. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki.  (2006-03-24)  Human chromosome 11 DNA sequence and analysis including novel gene identification..  Nature,  440  ((7083)): (497-500).  [PMID:16554811]
3. Phillips, J H JH and 5 more authors..  (1992)  Ontogeny of human natural killer (NK) cells: fetal NK cells mediate cytolytic function and express cytoplasmic CD3 epsilon,delta proteins..  The Journal of experimental medicine,  (1): [PMID:1372642]
4. Barber, E K EK, Dasgupta, J D JD, Schlossman, S F SF, Trevillyan, J M JM and Rudd, C E CE..  (1989)  The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:2470098]
5. Alexander, D D and 5 more authors..  (1989)  Dephosphorylation of the human T lymphocyte CD3 antigen..  European journal of biochemistry,  (15): [PMID:2540970]
6. van den Elsen, P P, Georgopoulos, K K, Shepley, B A BA, Orkin, S S and Terhorst, C C..  (1986)  Exon/intron organization of the genes coding for the delta chains of the human and murine T-cell receptor/T3 complex..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:2939461]
7. Tunnacliffe, A A, Sims, J E JE and Rabbitts, T H TH..  (1986)  T3 delta pre-mRNA is transcribed from a non-TATA promoter and is alternatively spliced in human T cells..  The EMBO journal,  [PMID:3488209]
8. van den Elsen, P P and 6 more authors..  (1984)  Isolation of cDNA clones encoding the 20K T3 glycoprotein of human T-cell receptor complex..  Nature,  [PMID:6095101]
9. Wilde, M I MI and Goa, K L KL..  (1996)  Muromonab CD3: a reappraisal of its pharmacology and use as prophylaxis of solid organ transplant rejection..  Drugs,  [PMID:8861551]
10. Doucey, Marie-Agnès MA and 7 more authors..  (2003)  CD3 delta establishes a functional link between the T cell receptor and CD8..  The Journal of biological chemistry,  (31): [PMID:12215456]
11. Salomon, Arthur R AR and 10 more authors..  (2003)  Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry..  Proceedings of the National Academy of Sciences of the United States of America,  (21): [PMID:12522270]
12. Dadi, Harjit K HK, Simon, Amos J AJ and Roifman, Chaim M CM..  (2003)  Effect of CD3delta deficiency on maturation of alpha/beta and gamma/delta T-cell lineages in severe combined immunodeficiency..  The New England journal of medicine,  (6): [PMID:14602880]
13. Jin, Pei P and 7 more authors..  (2004)  PCR isolation and cloning of novel splice variant mRNAs from known drug target genes..  Genomics,  [PMID:15028279]
14. Brill, Laurence M LM and 5 more authors..  (2004)  Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry..  Analytical chemistry,  (15): [PMID:15144186]
15. Arnett, Kelly L KL, Harrison, Stephen C SC and Wiley, Don C DC..  (2004)  Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment..  Proceedings of the National Academy of Sciences of the United States of America,  (16): [PMID:15534202]
16. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,  (18): [PMID:19690332]
17. Yu, Grace P and 13 more authors..  (2011)  Genotype, phenotype, and outcomes of nine patients with T-B+NK+ SCID..  Pediatric transplantation,  [PMID:21883749]
18. Zugmaier, Gerhard G, Klinger, Matthias M, Schmidt, Margit M and Subklewe, Marion M..  (2015)  Clinical overview of anti-CD19 BiTE(®) and ex vivo data from anti-CD33 BiTE(®) as examples for retargeting T cells in hematologic malignancies..  Molecular immunology,  [PMID:25883042]

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