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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab094722-10μl |
10μl |
现货 ![]() |
| |
| Ab094722-50μl |
50μL |
现货 ![]() |
| |
| Ab094722-100μl |
100μL |
期货 ![]() |
| |
| Ab094722-1ml |
1ml |
期货 ![]() |
|
| 产品名称 | CD3D Mouse mAb |
|---|---|
| 别名 | CD3 delta单克隆抗体 | CD3D小鼠单克隆抗体 | CD3D小鼠单抗 |
| 英文别名 | CD3 antigen delta subunit antibody | CD3 delta antibody | CD3-delta antibody | CD3d antibody | CD3d antigen delta polypeptide (TiT3 complex) antibody | CD3d molecule antibody | CD3d molecule delta (CD3-TCR complex) antibody | CD3D_HUMAN antibody | IMD19 a |
| 规格或纯度 | 无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | CD3D |
| 种属反应性 | 人(Human),猪(Pig) |
| 免疫原 | Recombinant CD3D expressed in E.coli (AA 18-113). |
| 阳性对照 | WB: Jurkat and MOLT-4 cell lysates. IHC: Human small intestine and spleen tissues. |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| 克隆号 | C6 |
| Format | Whole IgG |
| 亚型 | Mouse IgG2b |
| 轻链亚型 | kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified + Protein G purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4 |
| 防腐剂 | 0.05% Proclin 300 |
| 浓度 | 见COA |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
CD3D Mouse mAb (Ab094722) - Western Blot
All lanes: CD3D Mouse mAb (Ab094722) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 19 kDa
Observed band size: 23 kDa
Exposure time: 20.0 s
CD3D Mouse mAb (Ab094722) - IHC
DAB staining on IHC
Sample: Human Small intestine Tissue
Primary Ab: 10 µg/mL CD3D Mouse mAb (Ab094722)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
CD3D Mouse mAb (Ab094722) - IHC
DAB staining on IHC
Sample: Human Spleen Tissue
Primary Ab: 10 µg/mL CD3D Mouse mAb (Ab094722)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
| 应用名称 | 稀释比例 |
|---|---|
| IHC | 5-20 µg/mL |
| WB |
0.01-2 µg/mL
|
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab094722 | |
| 分析证书 | Ab094722 | |
| 分析证书 | Ab094722 |
| 1. de Saint Basile G, Geissmann F, Flori E, Uring-Lambert B, Soudais C, Cavazzana-Calvo M, Durandy A, Jabado N, Fischer A, Le Deist F. (2004) Severe combined immunodeficiency caused by deficiency in either the delta or the epsilon subunit of CD3.. J Clin Invest, 114 (10): (1512-7). [PMID:15546002] |
| 2. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811] |
| 3. Phillips, J H JH and 5 more authors.. (1992) Ontogeny of human natural killer (NK) cells: fetal NK cells mediate cytolytic function and express cytoplasmic CD3 epsilon,delta proteins.. The Journal of experimental medicine, (1): [PMID:1372642] |
| 4. Barber, E K EK, Dasgupta, J D JD, Schlossman, S F SF, Trevillyan, J M JM and Rudd, C E CE.. (1989) The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2470098] |
| 5. Alexander, D D and 5 more authors.. (1989) Dephosphorylation of the human T lymphocyte CD3 antigen.. European journal of biochemistry, (15): [PMID:2540970] |
| 6. van den Elsen, P P, Georgopoulos, K K, Shepley, B A BA, Orkin, S S and Terhorst, C C.. (1986) Exon/intron organization of the genes coding for the delta chains of the human and murine T-cell receptor/T3 complex.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2939461] |
| 7. Tunnacliffe, A A, Sims, J E JE and Rabbitts, T H TH.. (1986) T3 delta pre-mRNA is transcribed from a non-TATA promoter and is alternatively spliced in human T cells.. The EMBO journal, [PMID:3488209] |
| 8. van den Elsen, P P and 6 more authors.. (1984) Isolation of cDNA clones encoding the 20K T3 glycoprotein of human T-cell receptor complex.. Nature, [PMID:6095101] |
| 9. Wilde, M I MI and Goa, K L KL.. (1996) Muromonab CD3: a reappraisal of its pharmacology and use as prophylaxis of solid organ transplant rejection.. Drugs, [PMID:8861551] |
| 10. Doucey, Marie-Agnès MA and 7 more authors.. (2003) CD3 delta establishes a functional link between the T cell receptor and CD8.. The Journal of biological chemistry, (31): [PMID:12215456] |
| 11. Salomon, Arthur R AR and 10 more authors.. (2003) Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry.. Proceedings of the National Academy of Sciences of the United States of America, (21): [PMID:12522270] |
| 12. Dadi, Harjit K HK, Simon, Amos J AJ and Roifman, Chaim M CM.. (2003) Effect of CD3delta deficiency on maturation of alpha/beta and gamma/delta T-cell lineages in severe combined immunodeficiency.. The New England journal of medicine, (6): [PMID:14602880] |
| 13. Jin, Pei P and 7 more authors.. (2004) PCR isolation and cloning of novel splice variant mRNAs from known drug target genes.. Genomics, [PMID:15028279] |
| 14. Brill, Laurence M LM and 5 more authors.. (2004) Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.. Analytical chemistry, (15): [PMID:15144186] |
| 15. Arnett, Kelly L KL, Harrison, Stephen C SC and Wiley, Don C DC.. (2004) Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment.. Proceedings of the National Academy of Sciences of the United States of America, (16): [PMID:15534202] |
| 16. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 17. Yu, Grace P and 13 more authors.. (2011) Genotype, phenotype, and outcomes of nine patients with T-B+NK+ SCID.. Pediatric transplantation, [PMID:21883749] |
| 18. Zugmaier, Gerhard G, Klinger, Matthias M, Schmidt, Margit M and Subklewe, Marion M.. (2015) Clinical overview of anti-CD19 BiTE(®) and ex vivo data from anti-CD33 BiTE(®) as examples for retargeting T cells in hematologic malignancies.. Molecular immunology, [PMID:25883042] |