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Annexin V/ANXA5 Mouse mAb

    应用:
  • IF/ICC
  • IHC
  • WB
  • 细胞凋亡
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human)
  • 亚型: Mouse IgG1
  • 克隆号: C13
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab088956-10μl
10μl 现货 Stock Image
Ab088956-50μl
50μL 现货 Stock Image
Ab088956-100μl
100μL 现货 Stock Image
Ab088956-1ml
1ml 期货 Stock Image

基本信息

产品名称 Annexin V/ANXA5 Mouse mAb
别名 Anti-Annexin V/ANXA5抗体 | 膜联蛋白A5抗体 | 膜联蛋白A5单克隆抗体 | Annexin V抗体 | Annexin V单克隆抗体 | ANXA5抗体 | ANXA5单克隆抗体
英文别名 Anchorin CII | annexin A5 | Annexin V | annexin-5 | ANX5 | ANXA5 | ANXV | Calphobindin I | Endonexin II | ENX2CBP-I | Lipocortin V | PAP-I | Placental anticoagulant protein 4 | Placental anticoagulant protein I | PP4 | Thromboplastin inhibitor | VAC-alpha
规格或纯度 无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
生化机理 This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
宿主种属 小鼠(Mouse)
特异性 ANXA5
种属反应性 人(Human)
免疫原 Recombinant ANXA5 expressed in E.coli (AA 1-320)
阳性对照 WB: HeLa, Hep G2, K562, HEK293, MDA-MB-231 and MCF7 cell lysates. IHC: Human small intestine, Human kidney, Human cerebrum, Human spleen, Human placenta, Human colon tissues. IF/ICC: HeLa cells.
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 C13
Format Whole IgG
亚型 Mouse IgG1
轻链亚型 kappa
SDS-PAGE 150 kDa
纯化方法 Protein A + Protein G purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂 0.05% Proclin 300
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

ANXA5 Tbio 膜联蛋白A5(Annexin A5) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

图片

Annexin V/ANXA5 Mouse mAb (Ab088956) - Western Blot
All lanes: Annexin V/ANXA5 Mouse mAb (Ab088956) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 33 kDa
Exposure time: 1.5 s

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Small intestine Tissue
Primary Ab: 40 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 40 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Cerebrum Tissue
Primary Ab: 40 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Spleen Tissue
Primary Ab: 40 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Placenta Tissue
Primary Ab: 40 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Colon Tissue
Primary Ab: 20 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Cerebrum Tissue
Primary Ab: 20 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Small intestine Tissue
Primary Ab: 20 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Annexin V/ANXA5 Mouse mAb (Ab088956) - IF/ICC
IF analysis of Annexin V/ANXA5 Mouse mAb (Ab088956) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Annexin V/ANXA5 Mouse mAb (Ab088956) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

应用

应用名称 稀释比例
WB

0.01-4 µg/mL

IHC

2-40µg/mL

IF/ICC

5 µg/mL

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批号(Lot Number) 证书类型 货号
ZJ25F0420186 分析证书 Ab088956
ZJ25F0420185 分析证书 Ab088956
ZJ25F0420184 分析证书 Ab088956
ZJ24F0505160 分析证书 Ab088956
ZJ24F0505158 分析证书 Ab088956
ZJ24F0505159 分析证书 Ab088956

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