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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab087545-10μl |
10μl |
现货 ![]() |
| |
| Ab087545-50μl |
50μL |
现货 ![]() |
| |
| Ab087545-100μl |
100μL |
现货 ![]() |
| |
| Ab087545-1ml |
1ml |
期货 ![]() |
|
| 产品名称 | Adiponectin Mouse mAb |
|---|---|
| 别名 | 脂联素(ADPN)单克隆抗体 | 脂联素小鼠单克隆抗体 | 脂联素小鼠单抗 |
| 英文别名 | 30 kDa adipocyte complement related protein antibody | 30 kDa adipocyte complement-related protein antibody | ACDC antibody | Acrp 30 antibody | ACRP30 antibody | ADIPO_HUMAN antibody | Adipocyte antibody | Adipocyte C1q and collagen domain containing pro |
| 规格或纯度 | 无载体, ExactAb™, 已验证, 高性能, 见COA |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | ADIPOQ |
| 种属反应性 | 人(Human),小鼠(Mouse) |
| 免疫原 | Recombinant ADPN expressed in E.coli (AA 108-244) |
| 阳性对照 | WB: Mouse skeletal muscle lysate. IHC: Human cardiac muscle, Rat cardiac muscle tissues. |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| 克隆号 | H2 |
| Format | Whole IgG |
| 亚型 | Mouse IgG2b |
| 轻链亚型 | kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A + Protein G purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH7.4 |
| 防腐剂 | 0.02% Sodium azide |
| 浓度 | 见COA |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
Adiponectin Mouse mAb (Ab087545) - Western Blot
All lanes: Adiponectin Mouse mAb (Ab087545) at 1/2000 dilution
Samples: Lysates at 5 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 28 kDa
Exposure time: 50.0 s
Adiponectin Mouse mAb (Ab087545) - IHC
DAB staining on IHC
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20 µg/mL Adiponectin Mouse mAb (Ab087545)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Adiponectin Mouse mAb (Ab087545) - IHC
DAB staining on IHC
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20 µg/mL Adiponectin Mouse mAb (Ab087545)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Adiponectin Mouse mAb (Ab087545) - IHC
DAB staining on IHC
Sample: Rat Cardiac Muscle Tissue
Primary Ab: 30 µg/mL Adiponectin Mouse mAb (Ab087545)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
| 应用名称 | 稀释比例 |
|---|---|
| IHC | 5-30 µg/mL |
| WB |
0.01-2 µg/mL
|
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab087545 | |
| 分析证书 | Ab087545 | |
| 分析证书 | Ab087545 | |
| 分析证书 | Ab087545 | |
| 分析证书 | Ab087545 |
¥399.92
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| 2. Nakano, Y Y, Tobe, T T, Choi-Miura, N H NH, Mazda, T T and Tomita, M M.. (1996) Isolation and characterization of GBP28, a novel gelatin-binding protein purified from human plasma.. Journal of biochemistry, [PMID:8947845] |
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| 7. Ouchi, N N and 14 more authors.. (2000) Adiponectin, an adipocyte-derived plasma protein, inhibits endothelial NF-kappaB signaling through a cAMP-dependent pathway.. Circulation, (12): [PMID:10982546] |
| 8. Yamauchi, T T and 24 more authors.. (2001) The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity.. Nature medicine, [PMID:11479627] |
| 9. Hara, Kazuo K and 23 more authors.. (2002) Genetic variation in the gene encoding adiponectin is associated with an increased risk of type 2 diabetes in the Japanese population.. Diabetes, [PMID:11812766] |
| 10. Kondo, Hidehiko H and 11 more authors.. (2002) Association of adiponectin mutation with type 2 diabetes: a candidate gene for the insulin resistance syndrome.. Diabetes, [PMID:12086969] |
| 11. Vasseur, Francis F and 14 more authors.. (2002) Single-nucleotide polymorphism haplotypes in the both proximal promoter and exon 3 of the APM1 gene modulate adipocyte-secreted adiponectin hormone levels and contribute to the genetic risk for type 2 diabetes in French Caucasians.. Human molecular genetics, (1): [PMID:12354786] |
| 12. Waki, Hironori H and 12 more authors.. (2003) Impaired multimerization of human adiponectin mutants associated with diabetes. Molecular structure and multimer formation of adiponectin.. The Journal of biological chemistry, (10): [PMID:12878598] |
| 13. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161] |
| 14. Richards, Ayanthi A AA and 6 more authors.. (2006) Adiponectin multimerization is dependent on conserved lysines in the collagenous domain: evidence for regulation of multimerization by alterations in posttranslational modifications.. Molecular endocrinology (Baltimore, Md.), [PMID:16497731] |
| 15. Richards, Ayanthi A AA and 13 more authors.. (2010) Sialic acid modification of adiponectin is not required for multimerization or secretion but determines half-life in circulation.. Molecular endocrinology (Baltimore, Md.), [PMID:19855092] |
| 16. Min, Xiaoshan X and 7 more authors.. (2012) Crystal structure of a single-chain trimer of human adiponectin globular domain.. FEBS letters, (23): [PMID:22449980] |
| 17. Rosenow, Anja A and 6 more authors.. (2012) Resveratrol-induced changes of the human adipocyte secretion profile.. Journal of proteome research, (7): [PMID:22905912] |