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Adiponectin Mouse mAb

    应用:
  • IHC
  • WB
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human), 小鼠(Mouse)
  • 亚型: Mouse IgG2b
  • 克隆号: H2
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab087545-10μl
10μl 现货 Stock Image
Ab087545-50μl
50μL 现货 Stock Image
Ab087545-100μl
100μL 现货 Stock Image
Ab087545-1ml
1ml 期货 Stock Image

基本信息

产品名称 Adiponectin Mouse mAb
别名 脂联素(ADPN)单克隆抗体 | 脂联素小鼠单克隆抗体 | 脂联素小鼠单抗
英文别名 30 kDa adipocyte complement related protein antibody | 30 kDa adipocyte complement-related protein antibody | ACDC antibody | Acrp 30 antibody | ACRP30 antibody | ADIPO_HUMAN antibody | Adipocyte antibody | Adipocyte C1q and collagen domain containing pro
规格或纯度 无载体, ExactAb™, 已验证, 高性能, 见COA
宿主种属 小鼠(Mouse)
特异性 ADIPOQ
种属反应性 人(Human),小鼠(Mouse)
免疫原 Recombinant ADPN expressed in E.coli (AA 108-244)
阳性对照 WB: Mouse skeletal muscle lysate. IHC: Human cardiac muscle, Rat cardiac muscle tissues.
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 H2
Format Whole IgG
亚型 Mouse IgG2b
轻链亚型 kappa
SDS-PAGE 150 kDa
纯化方法 Protein A + Protein G purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH7.4
防腐剂 0.02% Sodium azide
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

ADIPOQ Tbio 脂联素(Adiponectin) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

图片

Adiponectin Mouse mAb (Ab087545) - Western Blot
All lanes: Adiponectin Mouse mAb (Ab087545) at 1/2000 dilution
Samples: Lysates at 5 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 26 kDa
Observed band size: 28 kDa
Exposure time: 50.0 s 

Adiponectin Mouse mAb (Ab087545) - IHC
DAB staining on IHC
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20 µg/mL Adiponectin Mouse mAb (Ab087545)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Adiponectin Mouse mAb (Ab087545) - IHC
DAB staining on IHC
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20 µg/mL Adiponectin Mouse mAb (Ab087545)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Adiponectin Mouse mAb (Ab087545) - IHC
DAB staining on IHC
Sample: Rat Cardiac Muscle Tissue
Primary Ab: 30 µg/mL Adiponectin Mouse mAb (Ab087545)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

应用

应用名称 稀释比例
IHC

5-30 µg/mL

WB

0.01-2 µg/mL

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找到5个结果

批号(Lot Number) 证书类型 货号
ZJ23F1201912 分析证书 Ab087545
ZJ23F1201913 分析证书 Ab087545
ZJ23F1201914 分析证书 Ab087545
ZJ24F1011854 分析证书 Ab087545
ZJ24F1011774 分析证书 Ab087545

引用文献

1. Maeda K, Okubo K, Shimomura I, Funahashi T, Matsuzawa Y, Matsubara K.  (1996)  cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (AdiPose Most abundant Gene transcript 1)..  Biochem Biophys Res Commun,  221  (2): (286-9).  [PMID:8619847]
2. Nakano, Y Y, Tobe, T T, Choi-Miura, N H NH, Mazda, T T and Tomita, M M..  (1996)  Isolation and characterization of GBP28, a novel gelatin-binding protein purified from human plasma..  Journal of biochemistry,  [PMID:8947845]
3. Saito, K K and 8 more authors..  (1999)  Organization of the gene for gelatin-binding protein (GBP28)..  Gene,  (18): [PMID:10095105]
4. Schäffler, A A and 7 more authors..  (1999)  The human apM-1, an adipocyte-specific gene linked to the family of TNF's and to genes expressed in activated T cells, is mapped to chromosome 1q21.3-q23, a susceptibility locus identified for familial combined hyperlipidaemia (FCH)..  Biochemical and biophysical research communications,  (5): [PMID:10403784]
5. Takahashi, M M and 13 more authors..  (2000)  Genomic structure and mutations in adipose-specific gene, adiponectin..  International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity,  [PMID:10918532]
6. Yokota, T T and 10 more authors..  (2000)  Adiponectin, a new member of the family of soluble defense collagens, negatively regulates the growth of myelomonocytic progenitors and the functions of macrophages..  Blood,  (1): [PMID:10961870]
7. Ouchi, N N and 14 more authors..  (2000)  Adiponectin, an adipocyte-derived plasma protein, inhibits endothelial NF-kappaB signaling through a cAMP-dependent pathway..  Circulation,  (12): [PMID:10982546]
8. Yamauchi, T T and 24 more authors..  (2001)  The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity..  Nature medicine,  [PMID:11479627]
9. Hara, Kazuo K and 23 more authors..  (2002)  Genetic variation in the gene encoding adiponectin is associated with an increased risk of type 2 diabetes in the Japanese population..  Diabetes,  [PMID:11812766]
10. Kondo, Hidehiko H and 11 more authors..  (2002)  Association of adiponectin mutation with type 2 diabetes: a candidate gene for the insulin resistance syndrome..  Diabetes,  [PMID:12086969]
11. Vasseur, Francis F and 14 more authors..  (2002)  Single-nucleotide polymorphism haplotypes in the both proximal promoter and exon 3 of the APM1 gene modulate adipocyte-secreted adiponectin hormone levels and contribute to the genetic risk for type 2 diabetes in French Caucasians..  Human molecular genetics,  (1): [PMID:12354786]
12. Waki, Hironori H and 12 more authors..  (2003)  Impaired multimerization of human adiponectin mutants associated with diabetes. Molecular structure and multimer formation of adiponectin..  The Journal of biological chemistry,  (10): [PMID:12878598]
13. Zhang, Zemin Z and Henzel, William J WJ..  (2004)  Signal peptide prediction based on analysis of experimentally verified cleavage sites..  Protein science : a publication of the Protein Society,  [PMID:15340161]
14. Richards, Ayanthi A AA and 6 more authors..  (2006)  Adiponectin multimerization is dependent on conserved lysines in the collagenous domain: evidence for regulation of multimerization by alterations in posttranslational modifications..  Molecular endocrinology (Baltimore, Md.),  [PMID:16497731]
15. Richards, Ayanthi A AA and 13 more authors..  (2010)  Sialic acid modification of adiponectin is not required for multimerization or secretion but determines half-life in circulation..  Molecular endocrinology (Baltimore, Md.),  [PMID:19855092]
16. Min, Xiaoshan X and 7 more authors..  (2012)  Crystal structure of a single-chain trimer of human adiponectin globular domain..  FEBS letters,  (23): [PMID:22449980]
17. Rosenow, Anja A and 6 more authors..  (2012)  Resveratrol-induced changes of the human adipocyte secretion profile..  Journal of proteome research,  (7): [PMID:22905912]

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