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UAP56 Antibody - Primary antibody, specific to DDX39B, Rabbit IgG

COA COA
    Application:
  • Flow Cytometry
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab133187
Grouped product items
SKU Size
Availability
 Price Qty
Ab133187-10μl
10μl
2
$66.90
Ab133187-50μl
50μl
1
$189.90
Ab133187-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$279.90
Ab133187-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

pAb; Rabbit anti Human UAP56 Antibody; WB, IHC, Flow; Unconjugated

View related series
Click to view target information: DDX39B
Accession#:Q13838 (2) DDX39B (2) Flow Cytometry (1158) Gene ID:7919 (2) UAP56 (2)

Basic Description

Product Name UAP56 Antibody - Primary antibody, specific to DDX39B, Rabbit IgG
Synonyms 0610030D10Rik antibody | 4F2-LC6 antibody | 56 kDa U2AF65-associated protein antibody | AI428441 antibody | ATP-dependent RNA helicase p47 antibody | B(0,+)-type amino acid transporter 1 antibody | BAT1 antibody | Bat1a antibody | D17H6S81E antibody | D17
Specifications & Purity ExactAb™, Validated, Carrier Free, 0.5 mg/mL
Host species Rabbit
Specificity DDX39B
Immunogen This BAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide from the C-terminal region of human BAT1. (AA 351-380).
Positive Control WB: K562, HeLa, A431, HEK293 and Jurkat cell lysates. IHC: Human kidney tissue. Flow: K562 cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Rabbit anti Human DDX39B Antibody, Polyclonal, could be used for WB, IHC, Flow and so on.
Application:
· WB: 1/1000
· IHC: 1/100-1/500
· Flow: 1/10-1/50
Protein Function
Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Protein A purified+ Peptide affinity purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

DDX39B Tbio Spliceosome RNA helicase DDX39B (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

UAP56 Antibody (Ab133187) - Western Blot
All lanes: UAP56 Antibody (Ab133187) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 35.4 s


UAP56 Antibody (Ab133187) - Western Blot
All lanes: UAP56 Antibody (Ab133187) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 16.5 s

UAP56 Antibody (Ab133187) - IHC
Formalin-fixed and paraffin-embedded human kidney reacted with UAP56 Antibody (Ab133187), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

UAP56 Antibody (Ab133187) - Flow Cytometry
UAP56 Antibody (Ab133187) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application

Application Dilution info
IHC

1/100-1/500
WB

1/1000

Flow Cytometry

1/10-1/50

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0303482 Certificate of Analysis Mar 21, 2024 Ab133187
ZJ24F0303481 Certificate of Analysis Mar 21, 2024 Ab133187

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