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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab133422-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$79.90
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Ab133422-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$219.90
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Ab133422-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$349.90
|
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Ab133422-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,399.90
|
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Recombinant; Rabbit anti Human UFM1 Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant UFM1 Antibody - Primary antibody, specific to UFM1, Rabbit IgG |
|---|---|
| Synonyms | BM002 antibody | C13orf20 antibody | Chromosome 13 open reading frame 20 antibody | Ubiquitin fold modifier 1 antibody | Ubiquitin-fold modifier 1 antibody | UFM1 antibody | UFM1_HUMAN antibody | bA131P10.1 | BM 002 antibody | BM-002 | C13orf20 | chromoso |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.9 mg/mL |
| Host species | Rabbit |
| Specificity | UFM1 |
| Immunogen | A synthetic peptide derived from human UFM1 (AA 71-85). |
| Positive Control | WB: Jurkat, Hep G2, MCF7, HEL and NIH/3T3 cell lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human UFM1 Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Shape | Liquid |
| Concentration | 0.9 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Recombinant UFM1 Antibody (Ab133422) - Western Blot
All lanes: Recombinant UFM1 Antibody (Ab133422) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 9 kDa
Observed band size: 12 kDa
Exposure time: 26.4 s
Recombinant UFM1 Antibody (Ab133422) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant UFM1 Antibody (Ab133422). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant UFM1 Antibody (Ab133422) - IF/ICC
IF analysis of Recombinant UFM1 Antibody (Ab133422) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant UFM1 Antibody (Ab133422) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab138205) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
| Application | Dilution info |
|---|---|
| IF/ICC |
1:500
|
| WB |
1/500-1/1000
|
| IHC | 1/50-1/100
|