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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab130770-10μl
|
10μl |
2
|
$69.90
|
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Ab130770-50μl
|
50μl |
1
|
$209.90
|
|
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Ab130770-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$309.90
|
|
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Ab130770-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,599.90
|
|
Recombinant; Rabbit anti Human TFEB Antibody; WB, IHC, IF/ICC; Unconjugated
| Product Name | Recombinant TFEB Antibody - Primary antibody, specific to TFEB, Rabbit IgG |
|---|---|
| Synonyms | Alpha TFEB antibody | AlphaTFEB antibody | bHLHe35 antibody | Class E basic helix-loop-helix protein 35 antibody | T cell transcription factor EB antibody | TCFEB antibody | TFEB antibody | TFEB_HUMAN antibody | Transcription factor EB antibody | ALPHATFE |
| Specifications & Purity | Recombinant, ExactAb™, Validated, See COA |
| Biochemical and Physiological Mechanisms | Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the express |
| Host species | Rabbit |
| Specificity | TFEB |
| Immunogen | Recombinant full length protein (Human). |
| Positive Control | WB: HeLa, Hep G2 and K562 cell lysates. IHC: Human lung cancer tissues. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human TFEB Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Recombinant TFEB Antibody (Ab130770) - Western Blot
All lanes: Recombinant TFEB Antibody (Ab130770) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 42, 61 kDa
Exposure time: 50.0 s
Recombinant TFEB Antibody (Ab130770) - IF/ICC
Immunocytochemistry analysis of Transcription Factor EB (green) in HeLa using Recombinant TFEB Antibody (Ab130770), and cells were counterstained with DAPI (blue).
Recombinant TFEB Antibody (Ab130770) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant TFEB Antibody (Ab130770). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IF/ICC |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Mar 22, 2024 | Ab130770 | |
| Certificate of Analysis | Mar 22, 2024 | Ab130770 |