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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab117492-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$69.90
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Ab117492-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$189.90
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Ab117492-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$309.90
|
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Ab117492-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,699.90
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Recombinant; Rabbit anti Human NEDD8 Antibody; WB; Unconjugated
| Product Name | Recombinant NEDD8 Antibody - Primary antibody, specific to NEDD8, Rabbit IgG |
|---|---|
| Synonyms | NEDD-8 | Neddylin | Neural precursor cell expressed developmentally down-regulated protein 8 | Ubiquitin-like protein Nedd8 | FLJ43224 antibody | MGC104393 antibody | MGC125896 antibody | MGC125897 antibody | NED8 antibody | NEDD 8 antibody | NEDD-8 antib |
| Specifications & Purity | Recombinant, ExactAb™, Validated, See COA |
| Biochemical and Physiological Mechanisms | Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins |
| Host species | Rabbit |
| Specificity | NEDD8 |
| Immunogen | A synthetic peptide derived from human NEDD8 (AA 1-40) |
| Positive Control | WB: K562, Jurkat, HeLa, THP-1, HEK293, NIH/3T3 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human NEDD8 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Recombinant NEDD8 Antibody (Ab117492) - Western Blot
All lanes: Recombinant NEDD8 Antibody (Ab117492) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 9 kDa
Observed band size: 10, 89 kDa
The band at 10 kDa represents free NEDD8 protein, while the band at 89 kDa corresponds to Cullin proteins that have undergone Neddylation modification. NEDD8 is a small protein that typically modifies Cullin proteins through covalent binding, resulting in the formation of Neddylated Cullins. This modification plays an important role in processes such as cell cycle regulation and protein degradation.
Exposure time: 4.2 s
| Application | Dilution info |
|---|---|
| WB |
1/1000-1/2000
|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Jan 07, 2025 | Ab117492 |