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Recombinant NEDD8 Antibody - Primary antibody, specific to NEDD8, Rabbit IgG

    Application:
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab117492
Grouped product items
SKU Size
Availability
Price Qty
Ab117492-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab117492-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$189.90
Ab117492-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab117492-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human NEDD8 Antibody; WB; Unconjugated

Basic Description

Product Name Recombinant NEDD8 Antibody - Primary antibody, specific to NEDD8, Rabbit IgG
Synonyms NEDD-8 | Neddylin | Neural precursor cell expressed developmentally down-regulated protein 8 | Ubiquitin-like protein Nedd8 | FLJ43224 antibody | MGC104393 antibody | MGC125896 antibody | MGC125897 antibody | NED8 antibody | NEDD 8 antibody | NEDD-8 antib
Specifications & Purity Recombinant, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins
Host species Rabbit
Specificity NEDD8
Immunogen A synthetic peptide derived from human NEDD8 (AA 1-40)
Positive Control WB: K562, Jurkat, HeLa, THP-1, HEK293, NIH/3T3 and C6 cell lysates.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human NEDD8 Antibody, Recombinant, could be used for WB and so on.

Product Properties

Isotype Rabbit IgG
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

NEDD8 Tchem NEDD8 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant NEDD8 Antibody (Ab117492) - Western Blot
All lanes: Recombinant NEDD8 Antibody (Ab117492) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 9 kDa
Observed band size: 10, 89 kDa
The band at 10 kDa represents free NEDD8 protein, while the band at 89 kDa corresponds to Cullin proteins that have undergone Neddylation modification. NEDD8 is a small protein that typically modifies Cullin proteins through covalent binding, resulting in the formation of Neddylated Cullins. This modification plays an important role in processes such as cell cycle regulation and protein degradation.
Exposure time: 4.2 s

Application

Application Dilution info
WB

1/1000-1/2000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

1 results found

Lot Number Certificate Type Date Item
ZJ25F0116661 Certificate of Analysis Jan 07, 2025 Ab117492

Solution Calculators

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