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Recombinant MEK2 Antibody - Primary antibody, specific to MAP2K2, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab114868
Grouped product items
SKU Size
Availability
 Price Qty
Ab114868-10μl
10μl
5
$69.90
Ab114868-50μl
50μl
2
$207.90
Ab114868-100μl
100μl
1
$297.90
Ab114868-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,697.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human MEK2 Antibody; WB, IHC, ICC, IF; Unconjugated

View related series
Click to view target information: MAP2K2
Accession#:P36507 (2) Gene ID:5605 (2) MAP2K2 (2) MEK2 (2)

Basic Description

Product Name Recombinant MEK2 Antibody - Primary antibody, specific to MAP2K2, Rabbit IgG
Synonyms MAP2K2 | MEK2 | MKK2 | PRKMK2 | Dual specificity mitogen-activated protein kinase kinase 2 | MAP kinase kinase 2 | MAPKK 2 | ERK activator kinase 2 | MAPK/ERK kinase 2 | MEK 2 | Cardiofaciocutaneous syndrome antibody | CFC syndrome antibody | CFC4 antibod
Specifications & Purity ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species Rabbit
Specificity MAP2K2
Immunogen A synthetic peptide derived from human MEK2 (AA 1-50).
Positive Control WB: A549, HeLa, Jurkat, A431, MCF7 cell lysates, Mouse brain, Mouse lung lysates; IHC: Human breast cancer; ICC/IF: HeLa
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human MEK2 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Immunogen affinity purified
Shape Liquid
Concentration 0.2 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

MAP2K2 Tclin Dual specificity mitogen-activated protein kinase kinase 2 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
MAP2K2 Tclin Dual specificity mitogen-activated protein kinase kinase 2 (1262 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
MAP2K2 Tclin Dual specificity mitogen-activated protein kinase kinase; MEK1/2 (426 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
MAP2K2 Tclin Protein cereblon/MAP2K2 (15 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
MAP2K2 Tclin VHL/MAP2K2 (53 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
MAP2K2 Tclin VHL-MAP2K1/MAP2K2 (64 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant MEK2 Antibody (Ab114868) - Western Blot
All lanes: Recombinant MEK2 Antibody (Ab114868) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 0.8 s

Recombinant MEK2 Antibody (Ab114868) - Western Blot
All lanes: Recombinant MEK2 Antibody (Ab114868) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 5.8 s

Recombinant MEK2 Antibody (Ab114868) - IF
Immunocytochemistry analysis of MEK2 (green) in HeLa cells using Recombinant MEK2 Antibody (Ab114868) and cells were counterstained with DAPI (blue).

Recombinant MEK2 Antibody (Ab114868) – IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant MEK2 Antibody (Ab114868). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
WB

1/500-1/1000
IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0800903 Certificate of Analysis Aug 25, 2023 Ab114868
ZJ23F0800904 Certificate of Analysis Aug 25, 2023 Ab114868

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