Grouped product items

SKU

Size

Availability

Price

Qty

Ab107657-10μl

10μl

Available within 8-12 weeks(?)

$49.90

Ab107657-50μl

50μl

Available within 8-12 weeks(?)

$149.90

Ab107657-100μl

100μl

Available within 8-12 weeks(?)

$239.90

Ab107657-1ml

1ml

Available within 8-12 weeks(?)

$2,199.90

Basic Description

Product Name	Recombinant Histone H2A.X Antibody - Primary antibody, specific to Histone H2A.X, Rabbit IgG
Synonyms	AW228881 antibody \| H2A histone family member X antibody \| H2A.FX antibody \| H2A.X antibody \| H2a/x antibody \| H2AFX antibody \| H2AX antibody \| H2AX histone antibody \| H2AX_HUMAN antibody \| Hist5.2ax antibody \| Histone 2A antibody \| Histone 2AX antibody \|
Specifications & Purity	Recombinant, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms	Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role i
Host species	Rabbit
Specificity	Histone H2A.X
Immunogen	A synthetic peptide derived from human Histone H2A.X (AA 12-48)
Positive Control	WB: Mouse kidney, Rat brain, Raji, Ramos, HeLa and HEK293 lysates. IHC: Rat stomach, pancreas, lung, Human Hodgkin's lymphoma, colon cancer, Mouse spleen tissues. IF/ICC: HeLa cells.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human Histone H2A.X Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

H2AFX Tbio Histone H2AX (0 Activities)

Discover Target: H2AFX

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: Recombinant Histone H2A.X Antibody (Ab107657)

Recombinant Histone H2A.X Antibody (Ab107657) - Western Blot
All lanes: Recombinant Histone H2A.X Antibody (Ab107657) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Monoubiquitination of Lys-120 (H2AXK119ub) by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression.The calculated molecular weight of H2AX is 15 kDa, but the ubiquitinated H2A.X is about 22 kDa.
Exposure time: 11.3 s

IF/ICC: Recombinant Histone H2A.X Antibody (Ab107657)

Recombinant Histone H2A.X Antibody (Ab107657) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Histone H2A.X Antibody (Ab107657).