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Recombinant eIF4E Antibody - Primary antibody, specific to EIF4E, Rabbit IgG

    Application:
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab101380
Grouped product items
SKU Size
Availability
Price Qty
Ab101380-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$66.90
Ab101380-50μl
50μl
1
$199.90
Ab101380-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab101380-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human eIF4E Antibody; WB, IHC; Unconjugated

Basic Description

Product Name Recombinant eIF4E Antibody - Primary antibody, specific to EIF4E, Rabbit IgG
Synonyms CBP | eIF4E | eIF-4E | EIF4E1 | EIF4EL1 | EIF4EL1MGC111573 | eIF-4F 25 kDa subunit | EIF4F | eukaryotic translation initiation factor 4E | eukaryotic translation initiation factor 4E-like 1 | mRNA cap-binding protein | AUTS19 antibody | CBP antibody | eIF
Specifications & Purity ExactAb™, Validated, Recombinant, 0.5 mg/mL
Host species Rabbit
Specificity EIF4E
Immunogen A synthetic peptide derived from human eIF4E (AA 176-194)
Positive Control WB: HeLa, K562, A431, HEK293, C6 and NIH/3T3 lysates. IHC: Mouse colon tissue.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human eIF4E Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Immunogen affinity purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

EIF4E Tchem Eukaryotic translation initiation factor 4E (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
EIF4E Tchem Eukaryotic translation initation factor (600 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
EIF4E Tchem Eukaryotic translation initiation factor 4E/Eukaryotic translation initiation factor 4E-binding protein 1 (15 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
EIF4E Tchem EIF4E/EIF4G1 (7 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant eIF4E Antibody (Ab101380) - Western Blot
All lanes: Recombinant eIF4E Antibody (Ab101380) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 27 kDa
Exposure time: 21.2 s

Recombinant eIF4E Antibody (Ab101380) - IHC
Immunohistochemistry analysis of paraffin-embedded mouse colon using Recombinant eIF4E Antibody (Ab101380). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
WB

1/500-1/1000

IHC

1/50 - 1/100

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1202239 Certificate of Analysis Dec 26, 2023 Ab101380
ZJ23F1202240 Certificate of Analysis Dec 26, 2023 Ab101380

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