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Recombinant Eg5 Antibody - Primary antibody, specific to Eg5, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab101062
Grouped product items
SKU Size
Availability
 Price Qty
Ab101062-10μl
10μl
2
$69.90
Ab101062-50μl
50μl
1
$199.90
Ab101062-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$279.90
Ab101062-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human Eg5 Antibody; WB, IHC, ICC, IF; Unconjugated

View related series
Click to view target information: KIF11
Accession#:P52732 (1) Eg5 (1) Gene ID:3832 (1) KIF11 (1)

Basic Description

Product Name Recombinant Eg5 Antibody - Primary antibody, specific to Eg5, Rabbit IgG
Synonyms EG5 | HKSP | KNSL1 | MCLMR | TRIP5 | EG5 antibody | HKSP antibody | KIF11 antibody | KIF11_HUMAN antibody | Kinesin family member 11 antibody | Kinesin like protein 1 antibody | Kinesin-like protein 1 antibody | Kinesin-like protein KIF11 antibody | Kines
Specifications & Purity ExactAb™, Validated, Recombinant, 0.6 mg/mL
Host species Rabbit
Specificity Eg5
Immunogen Recombinant protein of human Eg5 (AA 8-399)
Positive Control WB: HeLa, A549, U2OS, K562 and A431 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Eg5 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function：
Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Antigen affinity purified
Shape Liquid
Concentration 0.6 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

KIF11 Tchem Kinesin-like protein KIF11 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant Eg5 Antibody (Ab101062) - Western Blot
All lanes: Recombinant Eg5 Antibody (Ab101062) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 119 kDa
Observed band size: 121 kDa
Exposure time: 5.9 s

Recombinant Eg5 Antibody (Ab101062) - IF/ICC
Immunocytochemistry analysis of Eg5 (green) in HeLa using Recombinant Eg5 Antibody (Ab101062), and cells were counterstained with DAPI (blue).

Recombinant Eg5 Antibody (Ab101062) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant Eg5 Antibody (Ab101062). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
WB

1/500-1/1000
IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0404427 Certificate of Analysis Apr 11, 2024 Ab101062
ZJ24F0404426 Certificate of Analysis Apr 11, 2024 Ab101062

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