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Recombinant Cytokeratin 18 Antibody - Primary antibody, for WB,Flow,IHC,IP,IF/ICC various applications, from Human, generated in Rabbit, specific to KRT18, Rabbit IgG

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • IHC
  • IP
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Rat
  • Isotype: Rabbit IgG
  • Clone Number: RR693
  • Conjugation: Unconjugated
In stock
Item Number
Ab098863
Grouped product items
SKU Size
Availability
 Price Qty
Ab098863-10μl
10μl
5
$69.90
Ab098863-50μl
50μl
1
$207.90
Ab098863-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$297.90
Ab098863-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,517.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant (RR693); Rabbit anti Human Cytokeratin 18 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated

View related series
Click to view target information: KRT18
Accession#:P05783 (1) Cytokeratin 18 (1) Flow Cytometry (1158) Gene ID:3875 (1) KRT18 (1)

Basic Description

Product Name Recombinant Cytokeratin 18 Antibody - Primary antibody, for WB,Flow,IHC,IP,IF/ICC various applications, from Human, generated in Rabbit, specific to KRT18, Rabbit IgG
Synonyms Cell proliferation inducing gene 46 protein antibody | Cell proliferation inducing protein 46antibody | Cell proliferation-inducing gene 46 protein antibody | CK 18 antibody | CK-18 antibody | CK18 antibody | CYK 18 antibody | CYK18 antibody | Cytokeratin
Specifications & Purity ExactAb™, Validated, Recombinant, High performance, 0.5mg/mL
Host Species Rabbit
Host species Rabbit
Specificity KRT18
Application WB,Flow,IHC,IP,IF/ICC
Immunogen A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 18 was used as an immunogen.
Positive Control WB: Hela, A431, HepG2, T47D, MCF-7, A549 cell lysate; Rat Liver. IHC: Colonic adenocarcinoma tissue. Flow: HCT-116 cells. ICC/IF: HCT-116 cells. IP: Hela whole cell lysate.
Conjugation Unconjugated
Grade ExactAb™, High Performance, Recombinant, Validated
Product Description

Rabbit anti Human Cytokeratin 18 Antibody, Primary antibody, could be used for WB, IHC, Flow, IF/ICC, IP and so on.

Application

  • WB: 1/2000 - 1/5000     
  • IHC: 1/800 - 1/1600
  • Flow: 1/800 - 1/2000
  • IF/ICC: 1/800 - 1/2000
  • IP:  1/25

Function

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Post-translational

Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.

Product Properties

Antibody Type Primary antibody
Clonality Recombinant
Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Protein A purified
Source CHO supernatant
Shape Liquid
Concentration 0.5mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C for 2 years. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

KRT18 Tbio Keratin, type I cytoskeletal 18 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant Cytokeratin 18 Antibody (Ab098863) - Western Blot
All lanes: Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 44 kDa
Exposure time: 1.0 s

Recombinant Cytokeratin 18 Antibody (Ab098863) - ICC/IF
Recombinant Cytokeratin 18 Antibody (Ab098863) staining Cytokeratin 18 in HCT-116 cells by IF/ICC . Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1/2000) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An AF488- conjugated Goat Anti-Rabbit IgG (1/500).

Recombinant Cytokeratin 18 Antibody (Ab098863) - IHC
Immunohistochemistry analysis of colonic adenocarcinoma tissue labelling Cytokeratin 18 with Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Recombinant Cytokeratin 18 Antibody (Ab098863) - Flow Cytometry
Overlay histogram showing HCT-116 cells stained Cytokeratin 18 with Recombinant Cytokeratin 18 Antibody (Ab098863) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (Ab098863, 1/2000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

Recombinant Cytokeratin 18 Antibody (Ab098863) - IP
Cytokeratin 18 was immunoprecipitated from 0.4mg of HeLa whole cell lysate with Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/25 dilution.

Lane 1: Ab098863 IP in HeLa whole cell lysate
Lane 2: PBS instead of Ab098863 in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 μg (input)

Secondary: GAR HRP for IP 1:500
Exposure time: 20.0 s

Application

Application Dilution info
IF/ICC

1/800 - 1/2000
Flow Cytometry

1/800 - 1/2000
WB

1/2000 - 1/5000

IHC

1/800 - 1/1600

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0800786 Certificate of Analysis Aug 14, 2023 Ab098863
ZJ23F0800787 Certificate of Analysis Aug 14, 2023 Ab098863

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