Grouped product items

SKU

Size

Availability

Price

Qty

Ab097410-10μl

10μl

Available within 8-12 weeks(?)

$59.90

Ab097410-50μl

50μl

$177.90

Ab097410-100μl

100μl

$237.90

Ab097410-1ml

1ml

Available within 8-12 weeks(?)

$1,797.90

Basic Description

Product Name	Recombinant COX IV Antibody - Primary antibody, specific to COX4I1, Rabbit IgG
Synonyms	AL024441 antibody \| COX 4 antibody \| COX IV 1 antibody \| COX IV antibody \| COX IV-1 antibody \| Cox4 antibody \| COX41_HUMAN antibody \| Cox4a antibody \| COX4B antibody \| COX4I1 antibody \| COX4I2 antibody \| COX4L2 antibody \| COXIV antibody \| Cytochrome c oxi
Specifications & Purity	ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL
Host species	Rabbit
Specificity	COX4I1
Immunogen	A synthetic peptide corresponding to residues on the N-terminus of human COX IV was used as an immunogen.
Positive Control	WB: A431, HEK293, HeLa, Jurkat, MCF7, A549, THP-1, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Liver tissue. Flow: HeLa cells. IF/ICC: HeLa cells. IP: HeLa whole cell lysate.
Conjugation	Unconjugated
Grade	ExactAb™, High Performance, Recombinant, Validated
Product Description	Rabbit anti Human COX IV Antibody, Recombinant antibody (RR684), could be used for WB, IHC, Flow, IF, ICC, IP and so on. Application: WB: 1/5000 - 1/10000 IHC: 1/200 - 1/400 Flow: 1/200 - 1/1000 IF/ICC: 1/10 - 1/500 IP: 1/50

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Shape	Liquid
Concentration	0.5 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

COX4I1 Tbio Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (0 Activities)

Discover Target: COX4I1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: Recombinant COX IV Antibody (Ab097410)

Recombinant COX IV Antibody (Ab097410) - Western Blot
All lanes: Recombinant COX IV Antibody (Ab097410) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 17 kDa
Observed band size: 18 kDa
Exposure time: 15.1 s

IF: Recombinant COX IV Antibody (Ab097410)

Recombinant COX IV Antibody (Ab097410) - IF
IF analysis of COX IV (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

IHC: Recombinant COX IV Antibody (Ab097410)

Recombinant COX IV Antibody (Ab097410) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of liver tissue labelling COX IV with Recombinant COX IV Antibody (Ab097410) at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Flow Cytometry: Recombinant COX IV Antibody (Ab097410)

Recombinant COX IV Antibody (Ab097410) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant COX IV Antibody (Ab097410)(Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant COX IV Antibody (Ab097410) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

IP: Recombinant COX IV Antibody (Ab097410)

Recombinant COX IV Antibody (Ab097410) - IP
COX IV was immunoprecipitated from 0.4 mg of HeLa whole cell lysate with Recombinant COX IV Antibody (Ab097410) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500

Lane 1: Recombinant COX IV Antibody (Ab097410) IP in HeLa whole cell lysate
Lane 2: PBS instead of Recombinant COX IV Antibody (Ab097410) in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 μg (input).

Exposure: 20.0 s

IF: Recombinant COX IV Antibody (Ab097410)

Recombinant COX IV Antibody (Ab097410) - IF
IF analysis of COX IV (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (TRITC) (Ab176448) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

IF: Recombinant COX IV Antibody (Ab097410)

Recombinant COX IV Antibody (Ab097410) - IF
IF analysis of COX IV (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant COX IV Antibody (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ab176446) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Application

Application	Dilution info
IP	1/50
Flow Cytometry	1/200 - 1/1000
WB	1/5000 – 1/10000
IF/ICC	1/10 - 1/500

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0901104	Certificate of Analysis	Sep 18, 2023	Ab097410
ZJ23F0901105	Certificate of Analysis	Sep 18, 2023	Ab097410