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Recombinant CLIC4 Antibody - Primary antibody, specific to CLIC4, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab096857
Grouped product items
SKU Size
Availability
Price Qty
Ab096857-10μl
10μl
2
$69.90
Ab096857-50μl
50μl
1
$199.90
Ab096857-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab096857-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human CLIC4 Antibody; WB, IHC, IF, ICC; Unconjugated

Basic Description

Product Name Recombinant CLIC4 Antibody - Primary antibody, specific to CLIC4, Rabbit IgG
Synonyms MC3S5 | Chloride intracellular channel 4 | Chloride intracellular channel 4 (mitochondrial) | Chloride intracellular channel 4 like | Chloride intracellular channel protein 4 | Clic4 | CLIC4_HUMAN | CLIC4L | DKFZP566G223 | FLJ38640 | H1 antibody HUH1 | In
Specifications & Purity Recombinant, ExactAb™, Validated, KD, 0.5 mg/mL
Host species Rabbit
Specificity CLIC4
Immunogen A synthetic peptide derived from human CLIC4 (AA 3-16)
Positive Control WB: HeLa, HEK293, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human Cholangiocarcinoma tissue. ICC/IF: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, KD Validation, Recombinant, Validated
Product Description

Rabbit anti Human CLIC4 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Antigen affinity purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

CLIC4 Tbio Chloride intracellular channel protein 4 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant CLIC4 Antibody (Ab096857) - Western Blot
All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 29 kDa
Observed band size: 28 kDa
Exposure time: 21.9 s

Recombinant CLIC4 Antibody (Ab096857) - Western Blot
All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 29 kDa
Observed band size: 28 kDa
Exposure time: 35.0 s

Recombinant CLIC4 Antibody (Ab096857) - IF/ICC
Immunocytochemistry analysis of CLIC4 (green) in HeLa using Recombinant CLIC4 Antibody (Ab096857), and cells were counterstained with DAPI (blue). 

Recombinant CLIC4 Antibody (Ab096857) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant CLIC4 Antibody (Ab096857). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Recombinant CLIC4 Antibody (Ab096857) - Western Blot
All lanes: Recombinant CLIC4 Antibody (Ab096857) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 29 kDa
Observed band size: 28 kDa
Exposure time: 0.5 s

Application

Application Dilution info
WB

1/500-1/1000

IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0303176 Certificate of Analysis Mar 13, 2024 Ab096857
ZJ24F0303175 Certificate of Analysis Mar 13, 2024 Ab096857

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