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PKC zeta Antibody - Primary antibody, specific to PKC zeta, Rabbit IgG

    Application:
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Pig,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab122119
Grouped product items
SKU Size
Availability
Price Qty
Ab122119-10μl
10μl
1
$66.90
Ab122119-50μl
50μl
4
$199.90
Ab122119-100μl
100μl
3
$299.90
Ab122119-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

pAb; Rabbit anti Human PKC zeta Antibody; WB, IHC; Unconjugated

Basic Description

Product Name PKC zeta Antibody - Primary antibody, specific to PKC zeta, Rabbit IgG
Synonyms 14-3-3-zetaisoform antibody | AI098070 antibody | aPKCzeta antibody | C80388 antibody | EC 2.7.11.13 antibody | KPCZ_HUMAN antibody | nPKC zeta antibody | nPKC-zeta antibody | OTTHUMP00000001368 antibody | OTTHUMP00000044160 antibody | PKC 2 antibody | PK
Specifications & Purity Carrier Free, ExactAb™, Validated, High Performance, See COA
Host species Rabbit
Specificity PKC zeta
Immunogen Recombinant PKC zeta expressed in E.coli (AA 404-591)
Positive Control WB: A43, HEK293, Hep G2 and C6 cell lysates. IHC: Human cerebrum tissue.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, High Performance, Validated
Product Description

Rabbit anti Human PKC zeta Antibody, Polyclonal, could be used for WB, IHC and so on.

Application

WB: 0.01-2 µg/mL IHC: 5-20 µg/mL

Protein Function

This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Antigen affinity purified + Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

PRKCZ Tchem Protein kinase C zeta type (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

PKC zeta Antibody (Ab122119) - Western Blot
All lanes: PKC zeta Antibody (Ab122119) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 68 kDa
Observed band size: 46, 71, 78 kDa
Exposure time: 33.0 s

PKC zeta Antibody (Ab122119) - IHC
DAB staining on IHC
Samples: Human Cerebrum Tissue
Primary Ab: 20 μg/mL PKC zeta Antibody (Ab122119)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Application

Application Dilution info
IHC

5-20 µg/mL

WB

0.01-2 µg/mL

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1101705 Certificate of Analysis Nov 16, 2023 Ab122119
ZJ23F1101704 Certificate of Analysis Nov 16, 2023 Ab122119

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