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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab122119-10μl
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10μl |
1
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$66.90
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Ab122119-50μl
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50μl |
4
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$199.90
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Ab122119-100μl
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100μl |
3
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$299.90
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Ab122119-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$2,399.90
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pAb; Rabbit anti Human PKC zeta Antibody; WB, IHC; Unconjugated
| Product Name | PKC zeta Antibody - Primary antibody, specific to PKC zeta, Rabbit IgG |
|---|---|
| Synonyms | 14-3-3-zetaisoform antibody | AI098070 antibody | aPKCzeta antibody | C80388 antibody | EC 2.7.11.13 antibody | KPCZ_HUMAN antibody | nPKC zeta antibody | nPKC-zeta antibody | OTTHUMP00000001368 antibody | OTTHUMP00000044160 antibody | PKC 2 antibody | PK |
| Specifications & Purity | Carrier Free, ExactAb™, Validated, High Performance, See COA |
| Host species | Rabbit |
| Specificity | PKC zeta |
| Immunogen | Recombinant PKC zeta expressed in E.coli (AA 404-591) |
| Positive Control | WB: A43, HEK293, Hep G2 and C6 cell lysates. IHC: Human cerebrum tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Rabbit anti Human PKC zeta Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 0.01-2 µg/mL IHC: 5-20 µg/mL Protein Function This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified + Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
PKC zeta Antibody (Ab122119) - Western Blot
All lanes: PKC zeta Antibody (Ab122119) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 68 kDa
Observed band size: 46, 71, 78 kDa
Exposure time: 33.0 s
PKC zeta Antibody (Ab122119) - IHC
DAB staining on IHC
Samples: Human Cerebrum Tissue
Primary Ab: 20 μg/mL PKC zeta Antibody (Ab122119)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
| Application | Dilution info |
|---|---|
| IHC | 5-20 µg/mL |
| WB |
0.01-2 µg/mL
|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Nov 16, 2023 | Ab122119 | |
| Certificate of Analysis | Nov 16, 2023 | Ab122119 |