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Osteopontin Antibody - Primary antibody, specific to Osteopontin, Rabbit IgG

COA COA
    Application:
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Pig
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab119430
Grouped product items
SKU Size
Availability
 Price Qty
Ab119430-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$66.90
Ab119430-50μl
50μl
4
$189.90
Ab119430-100μl
100μl
3
$309.90
Ab119430-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,299.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

pAb; Rabbit anti Human Osteopontin Antibody; WB, IHC; Unconjugated

View related series
Click to view target information: SPP1
Accession#:P10451 (3) Gene ID:6696 (4) Osteopontin (4) SPP1 (5)

Basic Description

Product Name Osteopontin Antibody - Primary antibody, specific to Osteopontin, Rabbit IgG
Synonyms BNSP antibody | Bone sialoprotein 1 antibody | Bone sialoprotein I antibody | BSP I antibody | BSPI antibody | Early T lymphocyte activation 1 antibody | ETA 1 antibody | ETA1 antibody | MGC110940 antibody | Nephropontin antibody | OPN antibody | Osteopon
Specifications & Purity Carrier Free, ExactAb™, Validated, High Performance, See COA
Host species Rabbit
Specificity Osteopontin
Immunogen Recombinant Osteopontin expressed in E.coli (AA 17-287).
Positive Control WB: A549, Hep G2, HEK293, RAW 264.7 and NIH/3T3 cell lysates. IHC: Human kidney tissue.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, High Performance, Validated
Product Description

Rabbit anti Human Osteopontin Antibody, Polyclonal, could be used for WB, IHC and so on.

Application

WB: 0.01-3 µg/mL IHC: 5-30 µg/mL

Protein Function

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Antigen affinity purified + Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

SPP1 Tbio Osteopontin (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Osteopontin Antibody (Ab119430) - Western Blot
All lanes: Osteopontin Antibody (Ab119430) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 35 kDa
Observed band size: 32, 45 kDa
Exposure time: 15.6 s 

Osteopontin Antibody (Ab119430) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 µg/mL Osteopontin Antibody (Ab119430)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Application

Application Dilution info
IHC

5-30 µg/mL
WB

0.01-3 µg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1101832 Certificate of Analysis Nov 27, 2023 Ab119430
ZJ23F1101814 Certificate of Analysis Nov 27, 2023 Ab119430

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