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GABARAPL1 Antibody - Primary antibody, specific to GABARAPL1, Rabbit IgG

COA COA
    Application:
  • Flow Cytometry
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab104445
Grouped product items
SKU Size
Availability
 Price Qty
Ab104445-10μl
10μl
2
$76.90
Ab104445-50μl
50μl
1
$229.90
Ab104445-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$339.90
Ab104445-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

pAb; Rabbit anti Human GABARAPL1 Antibody; WB, IHC, Flow; Unconjugated

View related series
Click to view target information: GABARAPL1
Accession#:Q9H0R8 (2) Flow Cytometry (1158) GABARAPL1 (2) Gene ID:23710 (2)

Basic Description

Product Name GABARAPL1 Antibody - Primary antibody, specific to GABARAPL1, Rabbit IgG
Synonyms APG8 like antibody | APG8L antibody | ATG8 antibody | ATG8B antibody | ATG8L antibody | Early estrogen regulated protein antibody | Early estrogen-regulated protein antibody | GABA antibody | GABA(A) receptor associated protein like 1 antibody | GABA(A) r
Specifications & Purity ExactAb™, Validated, Carrier Free, 0.5 mg/mL
Host species Rabbit
Specificity GABARAPL1
Immunogen Recombinant human GABARAPL1 protein (AA 1-117).
Positive Control WB: HeLa, HEK293, SK-N-SH, NIH/3T3 and C6 cell lysates. IHC: Human brain tissue. Flow: HeLa cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Rabbit anti Human GABARAPL1 Antibody, Polyclonal, could be used for WB, IHC, Flow and so on.
Application:
WB: 1/1000
IHC: 1/100-1/500
Flow: 1/10-1/50
Protein Function
Ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Post-translational
The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL1-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL1-II (By similarity). ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes. The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

GABARAPL1 Tbio Gamma-aminobutyric acid receptor-associated protein-like 1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

GABARAPL1 Antibody (Ab104445) - Western Blot
All lanes: GABARAPL1 Antibody (Ab104445) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 14 kDa
Observed band size: 16, 18 kDa
Exposure time: 100.0 s

GABARAPL1 Antibody (Ab104445) - Flow
GABARAPL1 Antibody (Ab104445) flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis

GABARAPL1 Antibody (Ab104445) - IHC
Formalin-fixed and paraffin-embedded human brain tissue reacted with GABARAPL1 Antibody (Ab104445), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Application

Application Dilution info
IHC

1/100-1/500
WB

1/1000

Flow Cytometry

1/10-1/50

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0202932 Certificate of Analysis Mar 01, 2024 Ab104445
ZJ24F0202933 Certificate of Analysis Mar 01, 2024 Ab104445

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