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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab104356-10μl
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10μl |
1
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$69.90
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Ab104356-50μl
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50μl |
1
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$199.90
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Ab104356-100μl
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100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$289.90
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Ab104356-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,999.90
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pAb; Rabbit anti Human GAB1 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | GAB1 Antibody - Primary antibody, specific to GAB1, Rabbit IgG |
|---|---|
| Synonyms | Gab 1 antibody | GAB1 antibody | GAB1_HUMAN antibody | GRB 2 associated binder 1 antibody | GRB 2 associated binding protein 1 antibody | GRB2 associated binding protein 1 isoform a antibody | GRB2 associated binding protein 1 isoform b antibody | GRB2-as |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | GAB1 |
| Immunogen | A synthetic peptide derived from human GAB1 |
| Positive Control | WB: HEK293, Hep G2 and K562 cell lysates. IHC: Human breast cancer tissue. IF/ICC: Hep G2 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human GAB1 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
GAB1 Antibody (Ab104356) - Western Blot
All lanes: GAB1 Antibody (Ab104356) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 107 kDa
Exposure time: 120.0 s
GAB1 Antibody (Ab104356) - IHC
Immunohistochemical analysis of GAB1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with GAB1 Antibody (Ab104356) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
GAB1 Antibody (Ab104356) - IF/ICC
Immunofluorescent analysis of GAB1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GAB1 Antibody (Ab104356) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
| Application | Dilution info |
|---|---|
| IHC | 1/100 - 1/200 |
| WB | 1/500-1/1000 |
| ICC/IF | 1/100-1/500 |