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Goat Anti-Mouse IgG H&L (FITC) - Secondary antibody

1 Citations COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • Host species: Goat
  • Reactive species: Mouse
  • Conjugation: FITC
In stock
Item Number
Ab156254
Grouped product items
SKU Size
Availability
 Price Qty
Ab156254-100μg
100μg
5
$29.90
Ab156254-1mg
1mg
1
$199.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Secondary Antibody; Goat Anti-Mouse IgG H&L (FITC); IF, Flow

View related series
CLARITY tissue clearing technology (64) FITC (181) Flow Cytometry (1158) Goat Anti-Mouse (14)

Basic Description

Product Name Goat Anti-Mouse IgG H&L (FITC) - Secondary antibody
Synonyms Goat Anti-Mouse IgG H&L Antibody (FITC) | Anti-Mouse IgG H&L Antibody (FITC) | Mouse IgG H&L Antibody (FITC) | FITC-conjugated Goat Anti-Mouse IgG (H+L) | Goat Anti Mouse IgG Antibody (FITC) | Anti-Mouse IgG Antibody (FITC) | Mouse IgG Antibody (FITC) | G
Specifications & Purity ExactAb™, Azide Free, Validated, Ex:498nm, Em:517nm, Pre-adsorbed, 1.0 mg/mL
Host species Goat
Immunogen Mouse IgG
Conjugation FITC
Excitation(nm) Blue Laser(488nm)
Emission(nm) 517nm
Ex/Em(nm) Ex:498nm;Em:517nm
Grade Azide Free, Em:517nm, Ex:498nm, ExactAb™, Pre-adsorbed, Validated
Product Description

Application
IF: 1:1000
Flow: 1:200-1:2000

Product Properties

Purification Method Antigen affinity purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

Goat Anti-Mouse IgG H&L (FITC) (Ab156254) - IF
IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Goat Anti-Mouse IgG H&L (FITC) (Ab156254) - Flow Cytometry
Flow analysis of beta Actin (red line) in Jurkat cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/5000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.

Application

Application Dilution info
IF/ICC

1:1000
Flow Cytometry

1/200-1/2000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1001207 Certificate of Analysis Jul 30, 2024 Ab156254
ZJ23F1001206 Certificate of Analysis Jul 30, 2024 Ab156254

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Citations of This Product

How to Cite Aladdin Scientific Products?
1. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu.  (2024)  Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method.  Food Bioscience,  61  (104936). 

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