Determine the necessary mass, volume, or concentration for preparing a solution.
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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab115835-1mg
|
1mg |
7
|
$117.90
|
|
|
Ab115835-2mg
|
2mg |
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
|
$147.90
|
|
|
Ab115835-10mg
|
10mg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$447.90
|
|
pAb; Goat Anti-Mouse IgG H&L Antibody; ELISA, LF, GICA; Unconjugated
| Product Name | Goat Anti-Mouse IgG H&L Antibody - Secondary antibody, for ELISA,LF,GICA various applications, from Mouse, generated in Goat |
|---|---|
| Synonyms | Goat Anti-Mouse IgG H&L Antibody | Goat Anti Mouse IgG H&L Antibody | Anti-Mouse IgG H&L Antibody | Anti Mouse IgG H&L Antibody | Mouse IgG H&L Antibody | Goat Anti-Mouse IgG Antibody | Goat Anti Mouse IgG Antibody | Anti-Mouse IgG Antibody | Anti Mouse I |
| Specifications & Purity | Carrier Free, ExactAb™, Validated, See COA |
| Host Species | Goat |
| Host species | Goat |
| Application | ELISA,LF,GICA |
| Immunogen | Mouse IgG |
| Positive Control | Mouse IgG |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Goat Anti-Mouse IgG H&L Antibody, Secondary antibody, could be uesd for ELISA, LF, GICA and so on.
|
| Antibody Type | secondary antibody |
|---|---|
| Clonality | polyclonal antibodies |
| SDS-PAGE | 150kDa |
| Purification Method | Antigen affinity purified |
| Source | Serum |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C long term (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
Goat Anti-Mouse IgG H&L Antibody (Ab115835) - IF
IF analysis of beta Tubulin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L Antibody (Ab115835) conjugated with biotin at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at a dilution of 1/200 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Goat Anti-Mouse IgG H&L Antibody (Ab115835) - Flow Cytometry
Flow analysis of beta Actin (red line) in Jurkat cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/5000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L Antibody (Ab115835) conjugated with FITC (Fluorescein isothiocyanate) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.
| Application | Dilution info |
|---|---|
| ELISA | Use at an assay dependent concentration. |
| LF |
Use at an assay dependent concentration
|
| GICA | Use at an assay dependent concentration. |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | May 25, 2023 | Ab115835 | |
| Certificate of Analysis | May 25, 2023 | Ab115835 | |
| Certificate of Analysis | May 17, 2023 | Ab115835 | |
| Certificate of Analysis | May 17, 2023 | Ab115835 | |
| Certificate of Analysis | May 17, 2023 | Ab115835 |
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| 1. Xu Tao, Yang Changwei, Lu Yang, Wang Heng, Chen Cheng, Zhou Yuchen, Chen Xiaoqing. (2024) Photocrosslinkable human amniotic membrane hydrogel for recovery from spinal cord injury. Bio-Design and Manufacturing, (1-18). |