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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab097457-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$69.90
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Ab097457-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$199.90
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Ab097457-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$319.90
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Ab097457-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,499.90
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pAb; Rabbit anti Human COX4I2 Antibody; WB, IHC, IF/ICC; Unconjugated
| Product Name | COX4I2 Antibody - Primary antibody, specific to COX4I2, Rabbit IgG |
|---|---|
| Synonyms | COX IV-2 antibody | COX4 2 antibody | COX4 antibody | COX42_HUMAN antibody | COX4B antibody | Cox4i2 antibody | COX4L2 antibody | COXIV 2 antibody | Cytochrome c oxidase subunit 4 isoform 2 antibody | Cytochrome c oxidase subunit 4 isoform 2 mitochondrial |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. |
| Host species | Rabbit |
| Specificity | COX4I2 |
| Immunogen | A synthetic peptide derived from human COX4-2 |
| Positive Control | WB: Mouse heart, Rat heart lysates. IHC: Human Squamous cell carcinoma of lung tissue. IF/ICC: COS7 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human COX4I2 Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
COX4I2 Antibody (Ab097457) - Western Blot
All lanes: COX4I2 Antibody (Ab097457) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 20 kDa
Observed band size: 17 kDa
Exposure time: 120.0 s
COX4I2 Antibody (Ab097457) - IHC
Immunohistochemical analysis of COX4-2 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with COX4I2 Antibody (Ab097457) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
COX4I2 Antibody (Ab097457) - IF/ICC
Immunofluorescent analysis of COX4-2 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with COX4I2 Antibody (Ab097457) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
| Application | Dilution info |
|---|---|
| IHC | 1/50-1/200 |
| IF/ICC | 1/50-1/100 |
| WB |
1/500-1/2000
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