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COX4I2 Antibody - Primary antibody, specific to COX4I2, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab097457
Grouped product items
SKU Size
Availability
Price Qty
Ab097457-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab097457-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab097457-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$319.90
Ab097457-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,499.90

pAb; Rabbit anti Human COX4I2 Antibody; WB, IHC, IF/ICC; Unconjugated

Basic Description

Product Name COX4I2 Antibody - Primary antibody, specific to COX4I2, Rabbit IgG
Synonyms COX IV-2 antibody | COX4 2 antibody | COX4 antibody | COX42_HUMAN antibody | COX4B antibody | Cox4i2 antibody | COX4L2 antibody | COXIV 2 antibody | Cytochrome c oxidase subunit 4 isoform 2 antibody | Cytochrome c oxidase subunit 4 isoform 2 mitochondrial
Specifications & Purity ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Biochemical and Physiological Mechanisms This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Host species Rabbit
Specificity COX4I2
Immunogen A synthetic peptide derived from human COX4-2
Positive Control WB: Mouse heart, Rat heart lysates. IHC: Human Squamous cell carcinoma of lung tissue. IF/ICC: COS7 cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Rabbit anti Human COX4I2 Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Immunogen affinity purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

COX4I2 Tbio Cytochrome c oxidase subunit 4 isoform 2, mitochondrial (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

COX4I2 Antibody (Ab097457) - Western Blot
All lanes: COX4I2 Antibody (Ab097457) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 20 kDa
Observed band size: 17 kDa
Exposure time: 120.0 s

COX4I2 Antibody (Ab097457) - IHC
Immunohistochemical analysis of COX4-2 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with COX4I2 Antibody (Ab097457) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

COX4I2 Antibody (Ab097457) - IF/ICC
Immunofluorescent analysis of COX4-2 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with COX4I2 Antibody (Ab097457) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Application

Application Dilution info
IHC

1/50-1/200

IF/ICC

1/50-1/100

WB

1/500-1/2000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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