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Connexin 43/GJA1 (phospho S368) Antibody - Primary antibody, specific to GJA1 (phospho S368), Rabbit IgG

COA COA
    Application:
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab097298
Grouped product items
SKU Size
Availability
 Price Qty
Ab097298-10μl
10μl
1
$69.90
Ab097298-50μl
50μl
1
$199.90
Ab097298-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab097298-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

pAb; Rabbit anti Human Connexin 43/GJA1 (phospho S368) Antibody; WB, IHC; Unconjugated

View related series
Click to view target information: GJA1
Accession#: P17302 (3) Connexin 43 (2) Gene ID: 2697 (3) GJA1 (3) GJA1 (phospho S368) (1)

Basic Description

Product Name Connexin 43/GJA1 (phospho S368) Antibody - Primary antibody, specific to GJA1 (phospho S368), Rabbit IgG
Synonyms Connexin 43(phospho-Ser368) | Connexin 43(phospho Ser368) | Connexin 43(phospho S368) | Connexin 43 | Connexin43v Cx 43v CX43 | DFNB38 | Gap junction 43 kDa heart protein | Connexin-43 | Gap junction alpha 1 protein | Gap junction protein alpha 1 43kDa (c
Specifications & Purity Validated, 1.0 mg/mL
Host species Rabbit
Specificity GJA1 (phospho S368)
Immunogen A synthetic peptide derived from human Connexin 43/GJA1 (phospho S368) (AA 332-381).
Positive Control WB: K562 treated with 200ng/mL PMA for 10 min lysates. IHC: Human breast carcinoma tissue.
Conjugation Unconjugated
Grade Validated
Product Description

Rabbit polyclonal to Connexin 43/GJA1 (phospho S368), could be used for WB, IHC and so on.
Applications:
WB: 1/500 - 1/2000
IHC: 1/100 - 1/300
Protein Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150kDa
Purification Method Immunogen affinity purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

GJA1 Tbio Gap junction alpha-1 protein (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Connexin 43/GJA1 (phospho S368) Antibody (Ab097298) - Western Blot
All lanes: Connexin 43/GJA1 (phospho S368) Antibody (Ab097298) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 31, 37 kDa
The truncated isoforms are detected as distinct bands on the western blot lysate from the left ventricle of non-failing human heart tissue . In addition to 43 kDa full length isoform, the largest 3 isoforms (32 kDa, 29 kDa and 26 kDa in size) contain 2 transmembrane domains, part of intracellular loop, and the C-terminus. The smallest 3 isoforms are 20 kDa, 11 kDa, and 7 kDa in size and include part of the C-terminal tail with the 20 kDa isoform also containing part of the last transmembrane domain (PMID: 28576298).
Exposure time: 26.2 s

Connexin 43/GJA1 (phospho S368) Antibody (Ab097298) - IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Connexin 43/GJA1 (phospho S368) Antibody (Ab097298). The picture on the right is blocked with the phosphor peptide.

Application

Application Dilution info
WB

1/500-1/2000
IHC

1/100-1/300

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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