Plays a major role in tight junction-specific obliteration of the intercellular space.
Host species
Rabbit
Specificity
Claudin 5
Immunogen
A synthetic peptide derived from human Claudin 5 (AA 169-218).
Positive Control
WB: Mouse brain, Rat brain, Mouse lung, Rat kidney, A549 lysates. IHC: Human uterus, uterus cancer, liver cancer, lung cancer, stomach, stomach cancer and brain; Rat kidney; Mouse testis and brain tissues. IF/ICC: Human liver, lung, stomach tissues and A5
Conjugation
Unconjugated
Grade
ExactAb™, Validated
Product Description
Rabbit anti Human Claudin 5 Antibody, Polyclonal, could be used for WB, IHC and so on.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Shape
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Claudin 5 Antibody (Ab096723) - Western Blot All lanes: Claudin 5 Antibody (Ab096723) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 23 kDa Observed band size: 21 kDa Exposure time: 8.8 s
Claudin 5 Antibody (Ab096723) - Western Blot All lanes: Claudin 5 Antibody (Ab096723) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 23 kDa Observed band size: 21 kDa Exposure time: 8.8 s
Claudin 5 Antibody (Ab096723) - IF/ICC Immunofluorescence analysis of A549. Claudin 5 Antibody (Ab096723) (red) was diluted at 1/200 (4°C, overnight). Goat Anti Rabbit IgG (H&L) - AF594 Secondary antibody was diluted at 1/1000 (room temperature, 50min). DAPI (blue) 10 min.
Claudin 5 Antibody (Ab096723) - IHC Immunofluorescence analysis of human-lung tissue. Claudin 5 Antibody (Ab096723) (red) was diluted at 1/200 (4°C, overnight). Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).
Claudin 5 Antibody (Ab096723) - IHC Immunofluorescence analysis of human-lung tissue. Claudin 5 Antibody (Ab096723) (red) was diluted at 1/200 (4°C, overnight). Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).
Claudin 5 Antibody (Ab096723) - IHC Immunofluorescence analysis of human-stomach tissue. Claudin 5 Antibody (Ab096723) (red) was diluted at 1/200 (4°C, overnight). Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50min). Picture B: DAPI (blue) 10 min.
Claudin 5 Antibody (Ab096723) - IHC Immunofluorescence analysis of human-stomach tissue. Claudin 5 Antibody (Ab096723) (red) was diluted at 1/200 (4°C, overnight). Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min). Picture B: DAPI (blue) 10 min.
Claudin 5 Antibody (Ab096723) - IHC Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. Claudin 5 Antibody (Ab096723) was diluted at 1/200 (4°C, overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min). Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
Claudin 5 Antibody (Ab096723) - IHC Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. Claudin 5 Antibody (Ab096723) was diluted at 1/200 (4°C, overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min). Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
Claudin 5 Antibody (Ab096723) - IHC Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. Claudin 5 Antibody (Ab096723) was diluted at 1/200 (4°C, overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min). Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
Claudin 5 Antibody (Ab096723) - IHC Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. Claudin 5 Antibody (Ab096723) was diluted at 1/200 (4°C, overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min). Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
Claudin 5 Antibody (Ab096723) - IHC Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. Claudin 5 Antibody (Ab096723) was diluted at 1/200 (4°C, overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min). Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
Claudin 5 Antibody (Ab096723) - IHC Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. Claudin 5 Antibody (Ab096723) was diluted at 1/200 (4°C, overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min). Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
Claudin 5 Antibody (Ab096723) - IHC Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. Claudin 5 Antibody (Ab096723) was diluted at 1/200 (4°C, overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min). Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
Claudin 5 Antibody (Ab096723) - IHC Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. Claudin 5 Antibody (Ab096723) was diluted at 1/200 (4°C, overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min). Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
Claudin 5 Antibody (Ab096723) - IHC Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. Claudin 5 Antibody (Ab096723) was diluted at 1/200 (4°C, overnight). Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min). Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.
Claudin 5 Antibody (Ab096723) - IHC Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Claudin 5 Antibody (Ab096723). The picture on the right is blocked with the synthesized peptide.
