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Recombinant Melanoma gp100 Antibody - Primary antibody, specific to Melanoma gp100, Rabbit IgG

    Application:
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab114936
Grouped product items
SKU Size
Availability
Price Qty
Ab114936-10μl
10μl
2
$69.90
Ab114936-50μl
50μl
1
$199.90
Ab114936-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$319.90
Ab114936-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,099.90

Recombinant; Rabbit anti Human Melanoma gp100 Antibody; WB, IHC; Unconjugated

Basic Description

Product Name Recombinant Melanoma gp100 Antibody - Primary antibody, specific to Melanoma gp100, Rabbit IgG
Synonyms 95 kDa melanocyte specific secreted glycoprotein antibody | 95 kDa melanocyte-specific secreted glycoprotein antibody | D12S53E antibody | gp100 antibody | M-beta antibody | ME20 antibody | ME20 M/ME20 S antibody | ME20-M antibody | ME20-S antibody | ME20
Specifications & Purity Recombinant, ExactAb™, Validated, See COA
Host species Rabbit
Specificity Melanoma gp100
Immunogen A synthetic peptide derived from human Melanoma gp100 (AA 600-661).
Positive Control WB: Human melanoma lysates. IHC: Human melanoma tissue.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Melanoma gp100 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/2000
IHC: 1/50-1/200
Protein Function:
Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.

Product Properties

Isotype Rabbit IgG
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

PMEL Tclin Melanocyte protein PMEL (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant Melanoma gp100 Antibody (Ab114936) - Western Blot
Western blot analysis of Recombinant Melanoma gp100 Antibody (Ab114936) expression in human melanoma lysate.

Predicted band size: 70 kDa
Observed band size: 100 & 26 kDa
The gp100 protein exhibits different cleavage forms in melanoma cells. In one study (PMID: 15096515), gp100 was detected in the melanoma cell line MNT-1 as two major specific bands, measuring 100 kDa and 26 kDa.

Recombinant Melanoma gp100 Antibody (Ab114936) - IHC
Immunohistochemical analysis of paraffin-embedded human melanoma, using Recombinant Melanoma gp100 Antibody (Ab114936).

Application

Application Dilution info
WB

1/500-1/2000

IHC

1/50-1/200

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0708946 Certificate of Analysis Jul 29, 2024 Ab114936
ZJ24F0708945 Certificate of Analysis Jul 29, 2024 Ab114936

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