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ADPGK Antibody - Primary antibody, specific to ADPGK, Rabbit IgG

Grade & Purity:

1.0 mg/mL

Application:

IF/ICC
IHC
WB

Host species: Rabbit
Reactive species: Human,Monkey,Mouse,Rat
Isotype: Rabbit IgG
Conjugation: Unconjugated

In stock

Item Number

Ab087591

Grouped product items
SKU	Size	Availability	Price
Ab087591-10μl	10μl	1	$79.90
Ab087591-50μl	50μl	1	$229.90
Ab087591-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$349.90
Ab087591-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,299.90

pAb; Rabbit anti Human ADPGK Antibody; WB, IHC, IF, ICC; Unconjugated

View related series

Click to view target information: ADPGK

Accession#: Q9BRR6 (1) ADPGK (1) Gene ID: 83440 (1)

Basic Description

Product Name	ADPGK Antibody - Primary antibody, specific to ADPGK, Rabbit IgG
Synonyms	ADP dependent glucokinase antibody \| ADP GK antibody \| ADP-dependent glucokinase antibody \| ADP-GK antibody \| ADPGK antibody \| ADPGK_HUMAN antibody \| ATP dependent glucokinase antibody \| DKFZP434B195 antibody \| 2610017G09Rik antibody \| RbBP 35 antibody \|
Specifications & Purity	ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host species	Rabbit
Specificity	ADPGK
Immunogen	A KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ADPGK.
Positive Control	WB: HeLa cell lysate. IHC: Human breast cancer tissue. IF/ICC: HeLa cells.
Conjugation	Unconjugated
Grade	Carrier Free, ExactAb™, Validated
Product Description	Rabbit anti Human ADPGK Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/1000 IHC: 1/100-1/200 IF/ICC: 1/100-1/500 Protein Function Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Immunogen affinity purified
Shape	Liquid
Concentration	1.0 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

ADPGK Tbio ADP-dependent glucokinase (0 Activities)

Discover Target: ADPGK

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: ADPGK Antibody (Ab087591)

ADPGK Antibody (Ab087591) - Western Blot
All lanes: ADPGK Antibody (Ab087591) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 53 kDa
Observed band size: 42, 52 kDa
ADPGK Antibody (Ab087591) recognizes different isoforms of ADPGK.
Exposure time: 200.0 s

IHC: ADPGK Antibody (Ab087591)

ADPGK Antibody (Ab087591) - IHC
Immunohistochemical analysis of ADPGK staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ADPGK Antibody (Ab087591) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: ADPGK Antibody (Ab087591)

ADPGK Antibody (Ab087591) - IF/ICC
Immunofluorescent analysis of ADPGK staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ADPGK Antibody (Ab087591) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Application

Application	Dilution info
WB	1/500-1/1000
IF/ICC	1/100-1/500
IHC	1/200

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ADPGK Antibody - Primary antibody, specific to ADPGK, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

ADPGK Antibody - Primary antibody, specific to ADPGK, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews