基本信息

产品名称	Recombinant CD8a Antibody (APC)
别名	白细胞分化抗原8 \| 分化群8A \| CD8抗体 \| CD8a单抗 \| Anti-CD8a抗体
英文别名	alpha polypeptide (p32) antibody \| CD_antigen=CD8a antibody \| CD8 antibody \| CD8 antigen alpha polypeptide antibody \| CD8 antigen alpha polypeptide (p32) antibody \| CD8 antigen, alpha polypeptide (p32) antibody \| CD8a antibody \| CD8A antigen antibody \| CD
规格或纯度	ExactAb™, 已验证, 5 μL/test
生化机理	Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
宿主种属	小鼠(Mouse)
特异性	Human CD8a
种属反应性	人(Human)
免疫原	Human Peripheral Blood T Cells.
阳性对照	Flow Cytometry: Human peripheral blood lymphocytes
偶联	APC
Excitation(nm)	Red Laser(633nm)
Emission(nm)	660nm
Ex/Em(nm)	Ex:650nm;Em:660nm

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	5 μL/test
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

关联靶点（人）

CD8A Tbio T 细胞表面糖蛋白 CD8 α 链(T-cell surface glycoprotein CD8 alpha chain) (0 活性数据)

点击查看靶蛋白信息： CD8A

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Recombinant CD8a Antibody (APC) (Ab214457)

ecombinant CD8a Antibody (APC) (Ab214457) - Flow Cytometry
Flow cytometry analysis of CD8a expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD8a Antibody (APC) (Ab214457) 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (APC) (Ab180309).

Flow Cytometry: Recombinant CD8a Antibody (APC) (Ab214457)

Recombinant CD8a Antibody (APC) (Ab214457) - Flow Cytometry
Flow cytometry analysis of CD8a expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD8a Antibody (APC) (Ab214457) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (APC) (Ab180309). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.