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CD8 Mouse mAb

    应用:
  • Flow Cytometry
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human)
  • 亚型: Mouse IgG1
  • 克隆号: HIT8a
  • 偶联: Unconjugated
有货

库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab095357-10μg
10μg 现货 Stock Image
Ab095357-50μg
50μg 现货 Stock Image

基本信息

产品名称 CD8 Mouse mAb
别名 CD8单克隆抗体 | Anti-CD8a抗体 | CD8a抗体 | CD8a单抗 | CD8小鼠单克隆抗体 | CD8小鼠单抗
英文别名 CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | CD8 | CD8a molecule | CD8A | Leu2 T-lymphocyte antigen | LEU2 | MAL | OKT8 T-cell antigen | p32 | T cell co-receptor | T8 T-cell antigen | T-cell antigen Leu2 | T-cell surface glycoprotein CD8 alph
规格或纯度 无载体, ExactAb™, 已验证, 见COA
宿主种属 小鼠(Mouse)
特异性 CD8
种属反应性 人(Human)
免疫原 Tissue, cells or virus.
偶联 Unconjugated
备注 本产品即将售完下架,替代产品为 Ab210974

产品属性

克隆类型 单克隆抗体
克隆号 HIT8a
Format Whole IgG
亚型 Mouse IgG1
轻链亚型 kappa
SDS-PAGE 150 kDa
纯化方法 Protein A purified
物理外观 Liquid
储存缓冲液 10mM PBS, 0.1% Sodium azide, pH 7.2
防腐剂 0.1% Sodium azide
浓度 见COA
储存温度 2-8°C储存
运输条件 冰袋运输
稳定性与储存 4°C 下运输。4°C 长期保存(36 个月)。收货后建议分装。
分子类型 抗体

关联靶点(人)

CD8A Tbio T 细胞表面糖蛋白 CD8 α 链(T-cell surface glycoprotein CD8 alpha chain) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

图片

CD8 Mouse mAb (Ab095357) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD8 with CD8 Mouse mAb (Ab095357) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).

CD8 Mouse mAb (Ab095357) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD8 (red) with CD8 Mouse mAb (Ab095357) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称 稀释比例
Flow Cytometry

< 0.5µg/100w cells

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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ24F0202883 分析证书 Ab095357
ZJ24F0202882 分析证书 Ab095357
ZJ24F0202881 分析证书 Ab095357

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引用文献

1. Peter W Kim,Zhen-Yu J Sun,Stephen C Blacklow,Gerhard Wagner,Michael J Eck.  (2003-09-23)  A zinc clasp structure tethers Lck to T cell coreceptors CD4 and CD8..  Science (New York, N.Y.),  301  ((5640)): (1725-1728).  [PMID:14500983]
2. Leahy, D J DJ, Axel, R R and Hendrickson, W A WA..  (1992)  Crystal structure of a soluble form of the human T cell coreceptor CD8 at 2.6 A resolution..  Cell,  (20): [PMID:1547508]
3. Norment, A M AM, Lonberg, N N, Lacy, E E and Littman, D R DR..  (1989)  Alternatively spliced mRNA encodes a secreted form of human CD8 alpha. Characterization of the human CD8 alpha gene..  Journal of immunology (Baltimore, Md. : 1950),  (1): [PMID:2496167]
4. Nakayama, K K, Tokito, S S, Okumura, K K and Nakauchi, H H..  (1989)  Structure and expression of the gene encoding CD8 alpha chain (Leu-2/T8)..  Immunogenetics,  [PMID:2509342]
5. Giblin, P P, Ledbetter, J A JA and Kavathas, P P..  (1989)  A secreted form of the human lymphocyte cell surface molecule CD8 arises from alternative splicing..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:2536941]
6. Salter, R D RD and 6 more authors..  (1989)  Polymorphism in the alpha 3 domain of HLA-A molecules affects binding to CD8..  Nature,  (23): [PMID:2784196]
7. Littman, D R DR, Thomas, Y Y, Maddon, P J PJ, Chess, L L and Axel, R R..  (1985)  The isolation and sequence of the gene encoding T8: a molecule defining functional classes of T lymphocytes..  Cell,  [PMID:3871356]
8. Sukhatme, V P VP, Sizer, K C KC, Vollmer, A C AC, Hunkapiller, T T and Parnes, J R JR..  (1985)  The T cell differentiation antigen Leu-2/T8 is homologous to immunoglobulin and T cell receptor variable regions..  Cell,  [PMID:3918796]
9. Parnes, J R JR, Sizer, K C KC, Sukhatme, V P VP and Hunkapiller, T T..  (1985)  Structure of Leu-2/T8 as deduced from the sequence of a cDNA clone..  Behring Institute Mitteilungen,  [PMID:3936473]
10. Snow, P M PM and Terhorst, C C..  (1983)  The T8 antigen is a multimeric complex of two distinct subunits on human thymocytes but consists of homomultimeric forms on peripheral blood T lymphocytes..  The Journal of biological chemistry,  (10): [PMID:6605969]
11. Schlesinger, M M and 6 more authors..  (1994)  A distinctive form of soluble CD8 is secreted by stimulated CD8+ cells in HIV-1-infected and high-risk individuals..  Clinical immunology and immunopathology,  [PMID:7923932]
12. Gao, G F GF and 8 more authors..  (1997)  Crystal structure of the complex between human CD8alpha(alpha) and HLA-A2..  Nature,  (5): [PMID:9177355]
13. de la Calle-Martin, O O and 9 more authors..  (2001)  Familial CD8 deficiency due to a mutation in the CD8 alpha gene..  The Journal of clinical investigation,  [PMID:11435463]
14. Shiroishi, Mitsunori M and 13 more authors..  (2003)  Human inhibitory receptors Ig-like transcript 2 (ILT2) and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-G..  Proceedings of the National Academy of Sciences of the United States of America,  (22): [PMID:12853576]
15. Hillier, Ladeana W LW and 121 more authors..  (2005)  Generation and annotation of the DNA sequences of human chromosomes 2 and 4..  Nature,  (7): [PMID:15815621]
16. Pang, Dick John DJ, Hayday, Adrian C AC and Bijlmakers, Marie-José MJ..  (2007)  CD8 Raft localization is induced by its assembly into CD8alpha beta heterodimers, Not CD8alpha alpha homodimers..  The Journal of biological chemistry,  (4): [PMID:17341584]
17. Gibbings, Derrick J DJ, Marcet-Palacios, Marcelo M, Sekar, Yokananth Y, Ng, Marcus C Y MC and Befus, A Dean AD..  (2007)  CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses..  BMC immunology,  (1): [PMID:17678538]
18. Zhu, Jia and 11 more authors..  (2013)  Immune surveillance by CD8αα+ skin-resident T cells in human herpes virus infection..  Nature,  (23): [PMID:23657257]
19. Schuster, Philipp; Thomann, Sabrina; Werner, Maren; Vollmer, Jörg and Schmidt, Barbara..  (2015)  A subset of human plasmacytoid dendritic cells expresses CD8α upon exposure to herpes simplex virus type 1..  Frontiers in microbiology,  [PMID:26082771]

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