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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab169191-10μl |
10μl |
现货 ![]() |
| |
| Ab169191-50μl |
50μL |
现货 ![]() |
| |
| Ab169191-100μl |
100μL |
现货 ![]() |
| |
| Ab169191-1ml |
1ml |
期货 ![]() |
|
| 产品名称 | Cyclophilin B Mouse mAb |
|---|---|
| 别名 | 肽基脯氨酰异构酶 B | 亲环蛋白B | Cyclophilin B小鼠单克隆抗体 | Anti-Cyclophilin B抗体 | PPIB 抗体 | PPIB 小鼠单克隆抗体 | 环丝氨酸蛋白酶B小鼠单克隆抗体 | 环丝氨酸蛋白酶B小鼠单抗 |
| 英文别名 | AA408962 antibody | AA553318 antibody | AI844835 antibody | Cphn 2 antibody | Cphn2 antibody | Cyclophilin B antibody | Cyclophilin like protein antibody | CyP 20b antibody | CYP S1 antibody | CYP-S1 antibody | CYPB antibody | EC 5.2.1.8 antibody | MGC141 |
| 规格或纯度 | ExactAb™, Validated, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE), 1.0 mg/mL |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | Cyclophilin B |
| 种属反应性 | 人(Human),大鼠(Rat),小鼠(Mouse) |
| 预测反应种属 | Macaca thibetana thibetana,Pan paniscus,Pan troglodytes |
| 免疫原 | Recombinant protein corresponding to Human Cyclophilin B |
| 阳性对照 | WB: HeLa, MCF-7, HepG2, THP-1, U-2 OS, A549, K562, HEK293, NIH/3T3 and C6 Whole cell Lysates Flow: HeLa cells ICC/IF: U-2 OS cells |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| 克隆号 | EF01/1F1 |
| Format | Whole IgG |
| 亚型 | Mouse IgG1 |
| 轻链亚型 | kappa |
| SDS-PAGE | 150.0 KDa |
| 纯化方法 | Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | PBS, pH 7.2 |
| 浓度 | 1.0 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4°C 短期储存(1-2 周)。在 -20°C 或 -80°C 下保存。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
Cyclophilin B Mouse mAb (Ab169191) - Western Blot
All lanes: Cyclophilin B Mouse Antibody (Ab169191) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 22 kDa
Exposure time: 0.4 s
Cyclophilin B Mouse mAb (Ab169191) - Western Blot
All lanes: Cyclophilin B Mouse Antibody (Ab169191) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 20 kDa
Exposure time: 0.4 s
Cyclophilin B Mouse mAb (Ab169191) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Cyclophilin B Mouse mAb (Ab169191) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).
Cyclophilin B Mouse mAb (Ab169191) - ICC/IF
IF analysis of Cyclophilin B (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Cyclophilin B Mouse mAb (Ab169191) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| 应用名称 | 稀释比例 |
|---|---|
| IF/ICC |
1/200
|
| WB | 1/1,000 - 1/200,000; Predicted molecular weight: 23.7 KDa |
| Flow Cytometry |
1/100 - 1/1,6000
|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab169191 | |
| 分析证书 | Ab169191 | |
| 分析证书 | Ab169191 | |
| 分析证书 | Ab169191 | |
| 分析证书 | Ab169191 | |
| 分析证书 | Ab169191 | |
| 分析证书 | Ab169191 | |
| 分析证书 | Ab169191 | |
| 分析证书 | Ab169191 | |
| 分析证书 | Ab169191 | |
| 分析证书 | Ab169191 |
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