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Cyclophilin B Mouse mAb

    应用:
  • Flow Cytometry
  • IF/ICC
  • WB
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
  • 亚型: Mouse IgG1
  • 克隆号: EF01/1F1
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab169191-10μl
10μl 现货 Stock Image
Ab169191-50μl
50μL 现货 Stock Image
Ab169191-100μl
100μL 现货 Stock Image
Ab169191-1ml
1ml 期货 Stock Image

基本信息

产品名称 Cyclophilin B Mouse mAb
别名 肽基脯氨酰异构酶 B | 亲环蛋白B | Cyclophilin B小鼠单克隆抗体 | Anti-Cyclophilin B抗体 | PPIB 抗体 | PPIB 小鼠单克隆抗体 | 环丝氨酸蛋白酶B小鼠单克隆抗体 | 环丝氨酸蛋白酶B小鼠单抗
英文别名 AA408962 antibody | AA553318 antibody | AI844835 antibody | Cphn 2 antibody | Cphn2 antibody | Cyclophilin B antibody | Cyclophilin like protein antibody | CyP 20b antibody | CYP S1 antibody | CYP-S1 antibody | CYPB antibody | EC 5.2.1.8 antibody | MGC141
规格或纯度 ExactAb™, Validated, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE), 1.0 mg/mL
宿主种属 小鼠(Mouse)
特异性 Cyclophilin B
种属反应性 人(Human),大鼠(Rat),小鼠(Mouse)
预测反应种属 Macaca thibetana thibetana,Pan paniscus,Pan troglodytes
免疫原 Recombinant protein corresponding to Human Cyclophilin B
阳性对照 WB: HeLa, MCF-7, HepG2, THP-1, U-2 OS, A549, K562, HEK293, NIH/3T3 and C6 Whole cell Lysates Flow: HeLa cells ICC/IF: U-2 OS cells
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 EF01/1F1
Format Whole IgG
亚型 Mouse IgG1
轻链亚型 kappa
SDS-PAGE 150.0 KDa
纯化方法 Protein A purified
物理外观 Liquid
储存缓冲液 PBS, pH 7.2
浓度 1.0 mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 短期储存(1-2 周)。在 -20°C 或 -80°C 下保存。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

PPIB Tchem 肽基脯氨酰顺反异构酶B(Peptidyl-prolyl cis-trans isomerase B) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

图片

Cyclophilin B Mouse mAb (Ab169191) - Western Blot
All lanes: Cyclophilin B Mouse Antibody (Ab169191) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 24 kDa
Observed band size: 22 kDa
Exposure time: 0.4 s 

Cyclophilin B Mouse mAb (Ab169191) - Western Blot
All lanes: Cyclophilin B Mouse Antibody (Ab169191) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 24 kDa
Observed band size: 20 kDa
Exposure time: 0.4 s 

Cyclophilin B Mouse mAb (Ab169191) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Cyclophilin B Mouse mAb (Ab169191) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Cyclophilin B Mouse mAb (Ab169191) - ICC/IF
IF analysis of Cyclophilin B (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Cyclophilin B Mouse mAb (Ab169191) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

应用

应用名称 稀释比例
IF/ICC

1/200

WB

1/1,000 - 1/200,000; Predicted molecular weight: 23.7 KDa

Flow Cytometry

1/100 - 1/1,6000

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批号(Lot Number) 证书类型 货号
ZJ24F1114027 分析证书 Ab169191
ZJ24F1114026 分析证书 Ab169191
ZJ24F1114025 分析证书 Ab169191
ZJ24F0809945 分析证书 Ab169191
ZJ24F0809910 分析证书 Ab169191
ZJ24F0809908 分析证书 Ab169191
ZJ24F0809907 分析证书 Ab169191
ZJ24F0809909 分析证书 Ab169191
ZJ24F0506223 分析证书 Ab169191
ZJ24F0506222 分析证书 Ab169191
ZJ24F0506221 分析证书 Ab169191

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