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Hsp60 Mouse mAb

    应用:
  • Flow Cytometry
  • IF/ICC
  • WB
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human)
  • 亚型: Mouse IgG1
  • 克隆号: EF02/2E10
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab169190-10μl
10μl 现货 Stock Image
Ab169190-50μl
50μL 现货 Stock Image
Ab169190-100μl
100μL 现货 Stock Image
Ab169190-1ml
1ml 现货 Stock Image

基本信息

产品名称 Hsp60 Mouse mAb
别名 60 kD热休克蛋白 | 热休克蛋白60抗体 | 热激蛋白60 | Anti-Hsp60抗体 | Hsp60小鼠单克隆抗体 | Hsp60抗体 | Hsp60小鼠单克隆抗体 | Hsp60小鼠单抗
英文别名 60 kDa chaperonin antibody | 60 kDa heat shock protein, mitochondrial antibody | CH60_HUMAN antibody | Chaperonin 60 antibody | Chaperonin, 60-KD antibody | CPN60 antibody | fa04a05 antibody | GROEL antibody | heat shock 60kDa protein 1 (chaperonin) antib
规格或纯度 Validated, High performance, Carrier Free, Azide Free, ≥95%(SDS-PAGE), 1.0 mg/mL
宿主种属 小鼠(Mouse)
特异性 Hsp60
种属反应性 人(Human)
免疫原 Recombinant full length protein corresponding to Human Hsp60
阳性对照 WB: HEK293, HeLa, HepG2, A549, MOTL-4, MCF-7, Jurkat, K562, A431 and THP-1 Whole cell Lysates ICC/IF: U-2 OS cells Flow: Jurkat cells
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 EF02/2E10
Format Whole IgG
亚型 Mouse IgG1
轻链亚型 kappa
SDS-PAGE 150.0 KDa
纯化方法 Protein A purified
物理外观 Liquid
储存缓冲液 PBS, pH 7.2
浓度 1.0 mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 短期储存(1-2 周)。在 -20°C 或 -80°C 下保存。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

HSPD1 Tbio 60 kDa 热休克蛋白,线粒体(60 kDa heat shock protein, mitochondrial) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
HSPD1 Tbio Heat shock protein HSP 60 (26 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

图片

Hsp60 Mouse mAb (Ab169190) - Western Blot
All lanes: Hsp60 Mouse Antibody (Ab169190) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 58 kDa
Exposure time: 15.0 s 

Hsp60 Mouse mAb (Ab169190) - Western Blot
All lanes: Hsp60 Mouse Antibody (Ab169190) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 4.0 s 

Hsp60 Mouse mAb (Ab169190) - Flow Cytometry
Overlay histogram showing Jurkat cells stained with Hsp60 Mouse mAb (Ab169190) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Hsp60 Mouse mAb (Ab169190) - ICC/IF
IF analysis of Hsp60 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Hsp60 Mouse mAb (Ab169190) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

应用

应用名称 稀释比例
IF/ICC

1/200

Flow Cytometry

1/800 - 1/200,000

WB

1/1,000 - 1/200,000; Predicted molecular weight: 61.0 KDa

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批号(Lot Number) 证书类型 货号
ZJ24F1114044 分析证书 Ab169190
ZJ24F1114043 分析证书 Ab169190
ZJ24F1114042 分析证书 Ab169190
ZJ24F0809932 分析证书 Ab169190
ZJ24F0809906 分析证书 Ab169190
ZJ24F0809905 分析证书 Ab169190
ZJ24F0606531 分析证书 Ab169190
ZJ24F0606530 分析证书 Ab169190

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