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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab169190-10μl |
10μl |
现货 ![]() |
| |
| Ab169190-50μl |
50μL |
现货 ![]() |
| |
| Ab169190-100μl |
100μL |
现货 ![]() |
| |
| Ab169190-1ml |
1ml |
现货 ![]() |
|
| 产品名称 | Hsp60 Mouse mAb |
|---|---|
| 别名 | 60 kD热休克蛋白 | 热休克蛋白60抗体 | 热激蛋白60 | Anti-Hsp60抗体 | Hsp60小鼠单克隆抗体 | Hsp60抗体 | Hsp60小鼠单克隆抗体 | Hsp60小鼠单抗 |
| 英文别名 | 60 kDa chaperonin antibody | 60 kDa heat shock protein, mitochondrial antibody | CH60_HUMAN antibody | Chaperonin 60 antibody | Chaperonin, 60-KD antibody | CPN60 antibody | fa04a05 antibody | GROEL antibody | heat shock 60kDa protein 1 (chaperonin) antib |
| 规格或纯度 | Validated, High performance, Carrier Free, Azide Free, ≥95%(SDS-PAGE), 1.0 mg/mL |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | Hsp60 |
| 种属反应性 | 人(Human) |
| 免疫原 | Recombinant full length protein corresponding to Human Hsp60 |
| 阳性对照 | WB: HEK293, HeLa, HepG2, A549, MOTL-4, MCF-7, Jurkat, K562, A431 and THP-1 Whole cell Lysates ICC/IF: U-2 OS cells Flow: Jurkat cells |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| 克隆号 | EF02/2E10 |
| Format | Whole IgG |
| 亚型 | Mouse IgG1 |
| 轻链亚型 | kappa |
| SDS-PAGE | 150.0 KDa |
| 纯化方法 | Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | PBS, pH 7.2 |
| 浓度 | 1.0 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4°C 短期储存(1-2 周)。在 -20°C 或 -80°C 下保存。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
Hsp60 Mouse mAb (Ab169190) - Western Blot
All lanes: Hsp60 Mouse Antibody (Ab169190) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 58 kDa
Exposure time: 15.0 s
Hsp60 Mouse mAb (Ab169190) - Western Blot
All lanes: Hsp60 Mouse Antibody (Ab169190) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 4.0 s
Hsp60 Mouse mAb (Ab169190) - Flow Cytometry
Overlay histogram showing Jurkat cells stained with Hsp60 Mouse mAb (Ab169190) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).
Hsp60 Mouse mAb (Ab169190) - ICC/IF
IF analysis of Hsp60 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Hsp60 Mouse mAb (Ab169190) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| 应用名称 | 稀释比例 |
|---|---|
| IF/ICC |
1/200
|
| Flow Cytometry | 1/800 - 1/200,000
|
| WB |
1/1,000 - 1/200,000; Predicted molecular weight: 61.0 KDa
|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab169190 | |
| 分析证书 | Ab169190 | |
| 分析证书 | Ab169190 | |
| 分析证书 | Ab169190 | |
| 分析证书 | Ab169190 | |
| 分析证书 | Ab169190 | |
| 分析证书 | Ab169190 | |
| 分析证书 | Ab169190 |
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