Iba1 Mouse mAb

COA

级别和纯度:

≥95%(SDS-PAGE)
1.0 mg/mL

应用:

ELISA
Flow Cytometry
IF/ICC

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG2b
克隆号: AB05/2A5
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab156545-10μl	10μl	现货
Ab156545-50μl	50μL	现货
Ab156545-100μl	100μL	现货
Ab156545-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: AIF1

Accession#:P55008 (3) AIF1 (3) Gene ID:199 (3) Iba1 (3) 流式细胞术 (1163)

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基本信息

产品名称	Iba1 Mouse mAb
别名	离子化钙结合适配分子1 \| 离子化钙结合适配分子1抗体 \| 离子化钙结合适配分子1小鼠单克隆抗体 \| 小鼠抗人离子化钙结合适配分子1抗体 \| 同种异体移植炎症因子1 \| 同种异体移植炎症因子1抗体 \| 同种异体移植炎症因子1小鼠单克隆抗体 \| 小鼠抗人同种异体移植炎症因子1抗体 \| AIF1抗体 \| Iba1抗体 \| G1抗体
英文别名	AIF 1 antibody \| AIF-1 antibody \| Aif1 antibody \| AIF1 protein antibody \| AIF1_HUMAN antibody \| Allograft inflammatory factor 1 antibody \| Allograft inflammatory factor 1 splice variant G antibody \| allograft inflammatory factor-1 splice variant Hara-1 an
规格或纯度	ExactAb™, 已验证, 无动物源, 无载体, 无叠氮钠, 高性能, ≥95%(SDS-PAGE), 1.0 mg/mL
生化机理	Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
宿主种属	小鼠(Mouse)
特异性	Human Iba1
种属反应性	人(Human)
免疫原	Recombinant protein corresponding to Human Iba1
阳性对照	Flow Cytometry: THP-1 cells IF/ICC: U-2 OS cells
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
SDS-PAGE	150.0 KDa
纯化方法	Protein A purified
储存缓冲液	PBS, pH 7.2
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
分子类型	抗体

关联靶点（人）

AIF1 Tbio 同种异体移植炎症因子1(Allograft inflammatory factor 1) (0 活性数据)

点击查看靶蛋白信息： AIF1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Iba1 Mouse mAb (Ab156545)

Iba1 Mouse mAb (Ab156545) - Flow Cytometry 
Flow Cytometry analysis of THP-1 cells stained with Iba1 Mouse mAb (Ab156545) (Red) at 1.25 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

ICC/IF: Iba1 Mouse mAb (Ab156545)

Iba1 Mouse mAb (Ab156545) - ICC/IF
IF analysis of Iba1 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Iba1 Mouse mAb (Ab156545) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

应用