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UBE2M/UBC12 Mouse mAb

UBE2M/UBC12 Mouse mAb

COA

级别和纯度:

已验证

ExactAb™

见COA

应用:

IF/ICC
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse), 狗(Dog), 猴(Monkey)
亚型: Mouse IgG1
克隆号: OTI2D9
偶联: Unconjugated

有货

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货号 (SKU)	包装规格	是否现货
Ab133254-10μl	10μl	现货
Ab133254-50μl	50μL	现货
Ab133254-100μl	100μL	期货
Ab133254-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: UBE2M

Accession#: P61081 (2) Gene ID: 9040 (2) hUbc12 (1) UBC12 (1) UBE2M (2)

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基本信息

产品名称	UBE2M/UBC12 Mouse mAb
别名	UBC12多克隆抗体 \| UBC12多克隆抗体 \| 泛素蛋白连接酶M抗体 \| UBE2M/UBC12抗体 \| UBE2M/UBC12单克隆抗体
英文别名	hUbc12 antibody \| hUbc12 antibody \| NEDD8 carrier protein antibody \| NEDD8 carrier protein antibody \| NEDD8 conjugating enzyme Ubc12 antibody \| NEDD8 conjugating enzyme Ubc12 antibody \| NEDD8 protein ligase antibody \| NEDD8 protein ligase antibody \| NEDD8
规格或纯度	ExactAb™, 已验证, 见COA
生化机理	Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
宿主种属	小鼠(Mouse)
特异性	UBE2M/UBC12
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse),狗(Dog),猴(Monkey)
预测反应种属	牛(Cow)
免疫原	Recombinant full length protein of human UBE2M produced in HEK293T.
阳性对照	WB: Hep G2, HeLa, A549, HEK293, Ramos, NIH/3T3 and C6 cell lysates. IF/ICC: COS7 cells transfected by pCMV6-ENTRY UBE2M.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 1% BSA, 0.02% Sodium azide, pH 7.3
防腐剂	0.02% Sodium azide
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下长期保存（12 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

UBE2M Tchem NEDD8 结合酶 Ubc12(NEDD8-conjugating enzyme Ubc12) (0 活性数据)

点击查看靶蛋白信息： UBE2M

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

UBE2M Tchem NEDD8-conjugating enzyme Ubc12 (10 活性数据)

点击查看靶蛋白信息： UBE2M

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: UBE2M/UBC12 Mouse mAb (Ab133254)

UBE2M/UBC12 Mouse mAb (Ab133254) - Western Blot
All lanes: UBE2M/UBC12 Mouse mAb (Ab133254) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 20.0 s

IF/ICC: UBE2M/UBC12 Mouse mAb (Ab133254)

UBE2M/UBC12 Mouse mAb (Ab133254) - IF/ICC
UBE2M/UBC12 Mouse mAb (Ab133254) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2M.