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PDPK1 Mouse mAb

    应用:
  • ELISA
  • Flow Cytometry
  • IF/ICC
  • WB
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
  • 亚型: Mouse IgG1
  • 克隆号: 3H3D9
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab121091-10μl
10μl 现货 Stock Image
Ab121091-50μL
50μL 现货 Stock Image
Ab121091-100μL
100μL 现货 Stock Image
Ab121091-1mL
1ml 期货 Stock Image

基本信息

产品名称 PDPK1 Mouse mAb
别名 PDPK1 小鼠单克隆抗体 | PDPK1 小鼠单抗
英文别名 EC 2.7.11.1 | hPDK1
规格或纯度 ExactAb™, Validated, Carrier Free, 1.0mg/mL
宿主种属 小鼠(Mouse)
特异性 PDPK1
种属反应性 人(Human),大鼠(Rat),小鼠(Mouse)
免疫原 Purified recombinant fragment of human PDPK1 (AA: 457-556) expressed in E. Coli.
阳性对照 WB: C6, K562, NIH/3T3 and MCF7 cell lysates. Flow: HeLa cells. ICC/IF: HeLa cells.
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 3H3D9
Format Whole IgG
亚型 IgG1
轻链亚型 kappa
SDS-PAGE 150 kDa
纯化方法 Protein G purified
物理外观 Liquid
储存缓冲液 Purified antibody in 1xPBS with 0.05% sodium azide
防腐剂 0.05% sodium azid
浓度 1.0mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 下运输。在 +4°C 下短期储存(1-2 周)。收货后建议分装。长期储存于 -20°C。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

PDPK1 Tchem 3-磷酸肌醇依赖性蛋白激酶 1(3-phosphoinositide-dependent protein kinase 1) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

图片

PDPK1 Mouse mAb (Ab121091) - Western Blot
All lanes: PDPK1 Mouse mAb (Ab121091) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 64 kDa
Observed band size: 51- 64 kDa
Exposure time: 113.7 s


PDPK1 Mouse mAb (Ab121091) - ICC/IF
Immunofluorescence analysis of HeLa cells using PDPK1 Mouse mAb (Ab121091) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with AF555 phalloidin.

PDPK1 Mouse mAb (Ab121091) - Flow Cytometry
Flow cytometric analysis of HeLa cells using PDPK1 Mouse mAb (Ab121091) (green) and negative control (red).

应用

应用名称 稀释比例
IF/ICC

1/200-1/1000

Flow Cytometry

1/200-1/400

ELISA

1/10000

WB

1/500-1/2000.Predicted molecular weight:63 kDa.

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ24F1012038 分析证书 Ab121091
ZJ23F0700736 分析证书 Ab121091
ZJ23F0700737 分析证书 Ab121091

技术文档和文章

流式细胞术
流式细胞仪分析
如何选择和使用抗体 — 一抗
How to choose and use antibodies-primary antibody?
流式细胞术实验方案
How to choose and use antibodies-primary antibody?
How to choose and use antibodies-primary antibody?
采用碘化丙啶DNA染色法进行流式细胞术细胞周期分析
石蜡样品的免疫组化染色方案(IHC-P)
How to choose and use antibodies-primary antibody?
利用甲醇通透法进行流式细胞术
Flow Cytometry Using Methanol Permeabilization
Flow Cytometry Using Methanol Permeabilization

引用文献

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