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抗体
多克隆抗体
Galectin 1 Antibody

Galectin 1 Antibody

级别和纯度:

见COA

应用:

IHC
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型: Rabbit IgG
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货	价格	数量
Ab104554-10μl	10μl	现货
Ab104554-50μl	50μL	现货
Ab104554-100μl	100μL	现货
Ab104554-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: LGALS1

Accession#:P09382 (3) Galectin 1 (2) Gene ID:3956 (3) LGALS1 (3)

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基本信息

产品名称	Galectin 1 Antibody
别名	半乳糖凝集素1多克隆抗体 \| 半乳糖凝集素1抗体
英文别名	14 kDa laminin-binding protein antibody \| 14 kDa lectin antibody \| Beta galactoside binding lectin antibody \| Beta galactoside binding lectin L 14 I antibody \| beta galactoside binding protein antibody \| Beta-galactoside-binding lectin L-14-I antibody \| D
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属	兔(Rabbit)
特异性	Galectin 1
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	Recombinant Galectin 1 expressed in E.coli (AA 1-135)
阳性对照	WB: K562, A549, HUVEC, NIH/3T3 and C6 cell lysates. IHC: Human rectum and renal cancer tissues, Rat testis, colon and lung tissues, Porcine colon, uterus, small intestine, cardiac muscle and stomach tissues.
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Antigen affinity purified and Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂	0.05% Proclin 300
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

LGALS1 Tchem 半乳糖凝集素-1(Galectin-1) (0 活性数据)

点击查看靶蛋白信息： LGALS1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: Galectin 1 Antibody (Ab104554)

Galectin 1 Antibody (Ab104554) - Western Blot
All lanes: Galectin 1 Antibody (Ab104554) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 2.3 s

IHC: Galectin 1 Antibody (Ab104554)

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Samples: Human Rectum Tissue
Primary Ab: 20 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Galectin 1 Antibody (Ab104554)

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Samples: Human Renal cancer Tissue
Primary Ab: 20 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Galectin 1 Antibody (Ab104554)

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Sample: Rat Testis Tissue
Primary Ab: 10 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Galectin 1 Antibody (Ab104554)

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Sample: Rat Colon Tissue
Primary Ab: 10 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Galectin 1 Antibody (Ab104554)

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Sample: Rat Lung Tissue
Primary Ab: 10 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Galectin 1 Antibody (Ab104554)

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Sample: Porcine Colon Tissue
Primary Ab: 20 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Galectin 1 Antibody (Ab104554)

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Sample: Porcine Uterus Tissue
Primary Ab: 20 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Galectin 1 Antibody (Ab104554)

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Sample: Porcine Small intestine Tissue
Primary Ab: 20 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Galectin 1 Antibody (Ab104554)

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Sample: Porcine Cardiac Muscle Tissue
Primary Ab: 20 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Galectin 1 Antibody (Ab104554)

Galectin 1 Antibody (Ab104554) - IHC
DAB staining on IHC
Sample: Porcine Stomach Tissue
Primary Ab: 20 µg/mL Galectin 1 Antibody (Ab104554)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

应用

应用名称	稀释比例
IHC	5-20 µg/mL
WB	0.01-2 µg/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F1101527	分析证书	Ab104554
ZJ23F1101528	分析证书	Ab104554
ZJ24F1011834	分析证书	Ab104554

技术文档和文章

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化抗原修复

免疫肽封闭实验

Categories: 实验方案（Protocols）

探索主题:

多克隆抗体免疫肽封闭特异性结合

Immunopeptide blocking assay

Categories: 实验方案（Protocols）

探索主题:

Immunopeptideblocking PolyclonalAntibody SpecificBinding

Immunopeptide blocking assay

Categories: 实验方案（Protocols）

探索主题:

Immunopeptideblocking PolyclonalAntibody SpecificBinding

叠氮化钠去除实验

Categories: 实验方案（Protocols）

探索主题:

重组抗体叠氮化钠去除抗体纯化葡聚糖凝胶G-25 无叠氮化钠抗体

Sodium Azide Removal Protocol

Categories: 实验方案（Protocols）

探索主题:

Recombinant antibody Sodium Azide Removal Antibody Purification Sephadex G-25 Sodium Azide-Free Antibody

引用文献

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861]
2. Perillo NL, Pace KE, Seilhamer JJ, Baum LG. Apoptosis of T cells mediated by galectin-1.. Nature, [PMID:7501023]
3. Oberg CT, Blanchard H, Leffler H, Nilsson UJ.. (2008) Protein subtype-targeting through ligand epimerization: talose-selectivity of galectin-4 and galectin-8.. Bioorg Med Chem Lett, 18 (13): (3691-3694). [PMID:18539029] [10.1016/j.bmcl.2008.05.066]
4. Delaine T, Cumpstey I, Ingrassia L, Le Mercier M, Okechukwu P, Leffler H, Kiss R, Nilsson UJ.. (2008) Galectin-inhibitory thiodigalactoside ester derivatives have antimigratory effects in cultured lung and prostate cancer cells.. J Med Chem, 51 (24): (8109-8114). [PMID:19053747] [10.1021/jm801077j]
5. Dings RP, Miller MC, Nesmelova I, Astorgues-Xerri L, Kumar N, Serova M, Chen X, Raymond E, Hoye TR, Mayo KH.. (2012) Antitumor agent calixarene 0118 targets human galectin-1 as an allosteric inhibitor of carbohydrate binding.. J Med Chem, 55 (11): (5121-5129). [PMID:22575017] [10.1021/jm300014q]
6. Rajput VK, MacKinnon A, Mandal S, Collins P, Blanchard H, Leffler H, Sethi T, Schambye H, Mukhopadhyay B, Nilsson UJ.. (2016) A Selective Galactose-Coumarin-Derived Galectin-3 Inhibitor Demonstrates Involvement of Galectin-3-glycan Interactions in a Pulmonary Fibrosis Model.. J Med Chem, 59 (17): (8141-8147). [PMID:27500311] [10.1021/acs.jmedchem.6b00957]
7. Gabr M,Rehman AU,Chen HF. (2020) Quinoline-Pyrazole Scaffold as a Novel Ligand of Galectin-3 and Suppressor of TREM2 Signaling.. ACS Med Chem Lett, 11 (9): (1759-1765). [PMID:32944144] [10.1021/acsmedchemlett.0c00330]
8. Gitt, M A MA and Barondes, S H SH.. (1991) Genomic sequence and organization of two members of a human lectin gene family.. Biochemistry, (8): [PMID:1988031]
9. Sharma, A A, Chemelli, R R and Allen, H J HJ.. (1990) Human splenic galaptin: physicochemical characterization.. Biochemistry, (5): [PMID:2383549]
10. Abbott, W M WM and Feizi, T T.. (1989) Evidence that the 14 kDa soluble beta-galactoside-binding lectin in man is encoded by a single gene.. The Biochemical journal, (1): [PMID:2719646]
11. Hirabayashi, J J, Ayaki, H H, Soma, G G and Kasai, K K.. (1989) Cloning and nucleotide sequence of a full-length cDNA for human 14 kDa beta-galactoside-binding lectin.. Biochimica et biophysica acta, (1): [PMID:2719964]
12. Couraud, P O PO and 5 more authors.. (1989) Molecular cloning, characterization, and expression of a human 14-kDa lectin.. The Journal of biological chemistry, (15): [PMID:2910856]
13. Hirabayashi, J J and Kasai, K K.. (1988) Complete amino acid sequence of a beta-galactoside-binding lectin from human placenta.. Journal of biochemistry, [PMID:3065332]
14. Hirabayashi, J J, Kawasaki, H H, Suzuki, K K and Kasai, K K.. (1987) Further characterization and structural studies on human placenta lectin.. Journal of biochemistry, [PMID:3611046]
15. Dunham, I I and 24 more authors.. (1999) The DNA sequence of human chromosome 22.. Nature, (2): [PMID:10591208]
16. Fouillit, M M and 8 more authors.. (2000) Regulation of CD45-induced signaling by galectin-1 in Burkitt lymphoma B cells.. Glycobiology, [PMID:10764829]
17. Tinari, N N and 5 more authors.. (2001) Glycoprotein 90K/MAC-2BP interacts with galectin-1 and mediates galectin-1-induced cell aggregation.. International journal of cancer, (15): [PMID:11146440]
18. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801]
19. Matsuda, Akio A and 11 more authors.. (2003) Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways.. Oncogene, (22): [PMID:12761501]
20. He, Jiale J and Baum, Linda G LG.. (2004) Presentation of galectin-1 by extracellular matrix triggers T cell death.. The Journal of biological chemistry, (6): [PMID:14617626]
21. Collins, John E JE and 12 more authors.. (2004) A genome annotation-driven approach to cloning the human ORFeome.. Genome biology, [PMID:15461802]
22. Nishi, Nozomu N and 8 more authors.. (2008) Functional and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1.. Glycobiology, [PMID:18796645]
23. Than, Nandor Gabor NG and 17 more authors.. (2008) Emergence of hormonal and redox regulation of galectin-1 in placental mammals: implication in maternal-fetal immune tolerance.. Proceedings of the National Academy of Sciences of the United States of America, (14): [PMID:18824694]
24. Di Lella, Santiago S and 5 more authors.. (2009) Critical role of the solvent environment in galectin-1 binding to the disaccharide lactose.. Biochemistry, (3): [PMID:19128029]
25. Than, Nandor Gabor NG and 24 more authors.. (2009) A primate subfamily of galectins expressed at the maternal-fetal interface that promote immune cell death.. Proceedings of the National Academy of Sciences of the United States of America, (16): [PMID:19497882]
26. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895]
27. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378]
28. Rosenow, Anja A and 6 more authors.. (2012) Resveratrol-induced changes of the human adipocyte secretion profile.. Journal of proteome research, (7): [PMID:22905912]
29. Watson, Allison P AP, Evans, Rick L RL and Egland, Kristi A KA.. (2013) Multiple functions of sushi domain containing 2 (SUSD2) in breast tumorigenesis.. Molecular cancer research : MCR, [PMID:23131994]
30. Escoda-Ferran, Cristina C and 8 more authors.. (2014) Modulation of CD6 function through interaction with Galectin-1 and -3.. FEBS letters, (25): [PMID:24945728]
31. Tagliabracci, Vincent S VS and 14 more authors.. (2015) A Single Kinase Generates the Majority of the Secreted Phosphoproteome.. Cell, (18): [PMID:26091039]
32. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

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