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Goat Anti-Rabbit IgG H&L (AF405)

    应用:
  • IF/ICC
  • 宿主种属: 山羊(Goat)
  • 种属反应性: 兔(Rabbit)
  • 亚型: Goat IgG
  • 偶联: AF405
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab181953-10μg
10μg 现货 Stock Image
Ab181953-100μg
100μg 现货 Stock Image
Ab181953-1mg
1mg 现货 Stock Image

基本信息

产品名称 Goat Anti-Rabbit IgG H&L (AF405)
别名 羊抗兔IgG抗体 | 羊抗兔IgG | 山羊抗兔IgG | 山羊抗兔IgG抗体 | AF405标记的山羊抗兔IgG抗体 | 山羊抗兔 IgG H&L (AF405)
英文别名 Goat Anti-Rabbit IgG H&L Antibody | Goat Anti Rabbit IgG H&L Antibody | Anti-Rabbit IgG H&L Antibody | Anti Rabbit IgG H&L Antibody | Goat Anti-Rabbit IgG Antibody | Goat Anti Rabbit IgG Antibody | Anti- Rabbit IgG Antibody | Anti Rabbit IgG Antibody | AF
规格或纯度 ExactAb™, 已验证, Ex:401nm, Em:422nm, 1.0 mg/mL
生化机理 IgG 是血清和淋巴中含量最高的一类抗体。它能有效对抗细菌、真菌、病毒和外来颗粒。免疫球蛋白 G 抗体会触发补体系统的作用。
宿主种属 山羊(Goat)
种属反应性 兔(Rabbit)
免疫原 Rabbit IgG
偶联 AF405
Excitation(nm) Violet Laser(405nm)
Emission(nm) 422nm
Ex/Em(nm) Ex:401nm;Em:422nm

产品属性

克隆类型 多克隆抗体
Format Whole IgG
亚型 Goat IgG
纯化方法 antigen affinity chromatography
储存缓冲液 Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂 0.05% Proclin300
浓度 1.0 mg/mL
储存温度 避光,-20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 储存温度为 -20°C。避免冷冻/解冻循环。避免光照。
分子类型 抗体

图片

Goat Anti-Rabbit IgG H&L (AF405) (Ab181953) - IF
IF analysis of MMP9 (blue) in U2OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with MMP9 antibody (Ab115721) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF405) (Ab181953) at a dilution of 1/1000 for 1 hour at room temperature in the dark. beta Tubulin Mouse mAb (Ab179922) (green) was stained with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at 1/1000 dilution. Cells were counterstained with PI (red). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Goat Anti-Rabbit IgG H&L (AF405) (Ab181953) - IF
IF analysis of MMP9 (blue) in U2OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with MMP9 antibody (Ab115721) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF405) (Ab181953) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with PI (red). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

应用

应用名称 稀释比例
IF/ICC

1/100-1/1000

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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ24F0910381 分析证书 Ab181953
ZJ24F0910382 分析证书 Ab181953
ZJ24F0910383 分析证书 Ab181953

技术文档和文章

免疫荧光染色实验步骤(甲醇通透法)
Immunofluorescence Staining Protocol (Methanol permeabilization)
Immunofluorescence Staining Protocol (Methanol permeabilization)
蛋白质印迹的二抗选择指南
CLARITY组织透明化染色技术
转录因子流式细胞术的固定和通透方案
Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry
Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry
Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry

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