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CD79a Antibody

    应用:
  • Flow Cytometry
  • IHC
  • WB
  • 宿主种属: 兔(Rabbit)
  • 种属反应性: 人(Human)
  • 亚型: Rabbit IgG
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab095317-10μl
10μl 现货 Stock Image
Ab095317-50μl
50μL 现货 Stock Image
Ab095317-100μl
100μL 现货 Stock Image
Ab095317-1ml
1ml 期货 Stock Image

基本信息

产品名称 CD79a Antibody
别名 免疫球蛋白关联α (Iga) 多克隆抗体 | CD79a 抗体
英文别名 B lymphocyte-specific MB1 protein antibody | B-cell antigen receptor complex-associated protein alpha chain antibody | CD 79a antibody | CD79a antibody | CD79a antigen (immunoglobulin-associated alpha) antibody | CD79A antigen antibody | CD79a molecule, i
规格或纯度 无载体, ExactAb™, 已验证, 见COA
宿主种属 兔(Rabbit)
特异性 CD79a
种属反应性 人(Human)
免疫原 Recombinant CD79a expressed in E.coli (AA 36-219)
阳性对照 WB: Ramos and Raji cell lysates. IHC: Human lymphoma tissue. Flow: Human Raji Burkitt's lymphoma cell line.
偶联 Unconjugated

产品属性

克隆类型 多克隆抗体
Format Whole IgG
亚型 Rabbit IgG
SDS-PAGE 150 kDa
纯化方法 Antigen affinity purified and protein A purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH7.4
防腐剂 0.02% Sodium azide
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

CD79A Tbio B 细胞抗原受体复合物相关蛋白 α 链(B-cell antigen receptor complex-associated protein alpha chain) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

图片

CD79a Antibody (Ab095317) - Western Blot
All lanes: CD79a Antibody (Ab095317) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 25 kDa
Observed band size: 45 kDa
Exposure time: 10.0 s

CD79a Antibody (Ab095317) - IHC
DAB staining on IHC
Samples: Human Lymphoma Tissue
Primary Ab: 10 µg/mL CD79a Antibody (Ab095317)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

CD79a Antibody (Ab095317) - Flow Cytometry
Raji human Burkitt‘s lymphoma cell line was stained with CD79a Antibody (Ab095317) (filled red histogram) or Isotype control antibody (filled blue histogram), followed by CY5-conjugated Anti-rabbit IgG Secondary Antibody.

应用

应用名称 稀释比例
IHC

5-20 µg/mL

WB

0.5-2 µg/mL

Flow Cytometry

20μg/mL

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ23F1101595 分析证书 Ab095317
ZJ23F1101594 分析证书 Ab095317
ZJ24F1011832 分析证书 Ab095317

技术文档和文章

采用碘化丙啶DNA染色法进行流式细胞术细胞周期分析
流式实验样品制备实验指南
Experimental Guide of Sample Preparation for Flow Cytometry
冷冻样本的免疫组织化学染色实验方案(IHC-Fr)
免疫组织化学染色的抗原修复方案
Experimental Guide of Sample Preparation for Flow Cytometry
利用甲醇通透法进行流式细胞术
Flow Cytometry Using Methanol Permeabilization
Flow Cytometry Using Methanol Permeabilization

引用文献

1. Leduc, I I, Preud'homme, J L JL and Cogné, M M..  (1992)  Structure and expression of the mb-1 transcript in human lymphoid cells..  Clinical and experimental immunology,  [PMID:1395095]
2. Müller, B B, Cooper, L L and Terhorst, C C..  (1992)  Cloning and sequencing of the cDNA encoding the human homologue of the murine immunoglobulin-associated protein B29..  European journal of immunology,  [PMID:1534761]
3. Ha, H J HJ, Kubagawa, H H and Burrows, P D PD..  (1992)  Molecular cloning and expression pattern of a human gene homologous to the murine mb-1 gene..  Journal of immunology (Baltimore, Md. : 1950),  (1): [PMID:1538135]
4. Flaswinkel, H H and Reth, M M..  (1992)  Molecular cloning of the Ig-alpha subunit of the human B-cell antigen receptor complex..  Immunogenetics,  [PMID:1639443]
5. Yu, L M LM and Chang, T W TW..  (1992)  Human mb-1 gene: complete cDNA sequence and its expression in B cells bearing membrane Ig of various isotypes..  Journal of immunology (Baltimore, Md. : 1950),  (15): [PMID:1729378]
6. Sakaguchi, N N, Kashiwamura, S S, Kimoto, M M, Thalmann, P P and Melchers, F F..  (1988)  B lymphocyte lineage-restricted expression of mb-1, a gene with CD3-like structural properties..  The EMBO journal,  [PMID:2463161]
7. Vasile, S S, Coligan, J E JE, Yoshida, M M and Seon, B K BK..  (1994)  Isolation and chemical characterization of the human B29 and mb-1 proteins of the B cell antigen receptor complex..  Molecular immunology,  [PMID:7514267]
8. Hashimoto, S S, Chiorazzi, N N and Gregersen, P K PK..  (1995)  Alternative splicing of CD79a (Ig-alpha/mb-1) and CD79b (Ig-beta/B29) RNA transcripts in human B cells..  Molecular immunology,  [PMID:7643857]
9. Hashimoto, S S, Mohrenweiser, H W HW, Gregersen, P K PK and Chiorazzi, N N..  (1994)  Chromosomal localization, genomic structure, and allelic polymorphism of the human CD79 alpha (Ig-alpha/mb-1) gene..  Immunogenetics,  [PMID:7916003]
10. Ha, H H, Barnoski, B L BL, Sun, L L, Emanuel, B S BS and Burrows, P D PD..  (1994)  Structure, chromosomal localization, and methylation pattern of the human mb-1 gene..  Journal of immunology (Baltimore, Md. : 1950),  (15): [PMID:8207205]
11. Minegishi, Y Y and 5 more authors..  (1999)  Mutations in Igalpha (CD79a) result in a complete block in B-cell development..  The Journal of clinical investigation,  [PMID:10525050]
12. Gaul, B S BS, Harrison, M L ML, Geahlen, R L RL, Burton, R A RA and Post, C B CB..  (2000)  Substrate recognition by the Lyn protein-tyrosine kinase. NMR structure of the immunoreceptor tyrosine-based activation motif signaling region of the B cell antigen receptor..  The Journal of biological chemistry,  (26): [PMID:10748115]
13. Wang, Yue Y and 6 more authors..  (2002)  Novel Igalpha (CD79a) gene mutation in a Turkish patient with B cell-deficient agammaglobulinemia..  American journal of medical genetics,  (1): [PMID:11920841]

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