CD10 Mouse mAb

COA

级别和纯度:

无载体

已验证

ExactAb™

见COA

应用:

Flow Cytometry

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG1
克隆号: HI10a
偶联: Unconjugated

有货

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货号 (SKU)	包装规格	是否现货
Ab093617-10μg	10μg	现货
Ab093617-50μg	50μg	现货
Ab093617-100μg	100μg	现货
Ab093617-1mg	1mg	期货

查看相关系列

点击查看靶蛋白信息: MME

Accession#:P08473 (9) CD10 (8) Gene ID:4311 (9) MME (9) 流式细胞术 (1163)

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基本信息

产品名称	CD10 Mouse mAb
别名	CD10单克隆抗体 \| CD10抗体 \| CD10小鼠单克隆抗体 \| CD10小鼠单抗
英文别名	Atriopeptidase \| CALLA \| CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase) \| CD10 antigen \| CD10 \| CD10) \| CD10membrane metallo-endopeptidase variant 1 \| Common acute lymphocytic leukemia antigen \| DKFZp686O16152 \| EC 3.4.24 \| EC
规格或纯度	无载体, ExactAb™, 已验证, 见COA
宿主种属	小鼠(Mouse)
特异性	CD10
种属反应性	人(Human)
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	HI10a
Format	Whole IgG
亚型	Mouse IgG1
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 0.1% Sodium azide, pH 7.2
防腐剂	0.1% Sodium azide
浓度	见COA
储存温度	2-8°C储存
运输条件	冰袋运输
稳定性与储存	4°C 下运输。4°C 长期保存（36 个月）。收货后建议分装。
分子类型	抗体

关联靶点（人）

MME Tclin 脑啡肽酶(Neprilysin) (0 活性数据)

点击查看靶蛋白信息： MME

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Flow Cytometry: CD10 Mouse mAb (Ab093617)

CD10 Mouse mAb (Ab093617) - Flow Cytometry
Flow Cytometry analysis of LNCaP cells labelling CD10 (red) with CD10 Mouse mAb (Ab093617) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD10 Mouse mAb (Ab093617)

CD10 Mouse mAb (Ab093617) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD10 (red) with CD10 Mouse mAb (Ab093617) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	< 0.5µg/100w cells

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0102464	分析证书	Ab093617
ZJ24F0102463	分析证书	Ab093617
ZJ24F0102462	分析证书	Ab093617

技术文档和文章

利用甲醇通透法进行流式细胞术

Categories: 实验方案（Protocols）

探索主题:

流式细胞术流式抗体流式实验甲醇通透细胞分选细胞分型免疫研究细胞内抗原检测

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

可替换产品

引用文献

1. Fournié-Zaluski MC, Coric P, Turcaud S, Rousselet N, Gonzalez W, Barbe B, Pham I, Jullian N, Michel JB, Roques BP.. (1994) New dual inhibitors of neutral endopeptidase and angiotensin-converting enzyme: rational design, bioavailability, and pharmacological responses in experimental hypertension.. J Med Chem, 37 (8): (1070-1083). [PMID:8164250] [10.1021/jm00034a005]
2. Tsantili-Kakoulidou A, Nicolaou I, Vrakas D, Demopoulos VJ. (2005) Modelling of aldose reductase inhibitory activity of pyrrol-1-yl-acetic acid derivatives by means of multivariate statistics.. Med Chem, 1 (4): (321-6). [PMID:16789889]
3. Figueroa F, Davicino R, Micalizzi B, Oliveros L, Forneris M. (2012) Macrophage secretions modulate the steroidogenesis of polycystic ovary in rats: effect of testosterone on macrophage pro-inflammatory cytokines.. Life Sci, 90 (19-20): (733-9). [PMID:22480516]
4. Ruf S, Buning C, Schreuder H, Horstick G, Linz W, Olpp T, Pernerstorfer J, Hiss K, Kroll K, Kannt A et al.. (2012) Novel β-amino acid derivatives as inhibitors of cathepsin A.. J Med Chem, 55 (17): (7636-49). [PMID:22861813]
5. Deaton DN, Gao EN, Graham KP, Gross JW, Miller AB, Strelow JM. (2008) Thiol-based angiotensin-converting enzyme 2 inhibitors: P1 modifications for the exploration of the S1 subsite.. Bioorg Med Chem Lett, 18 (2): (732-7). [PMID:18078750]
6. Georgiadis D, Yiotakis A. (2008) Specific targeting of metzincin family members with small-molecule inhibitors: progress toward a multifarious challenge.. Bioorg Med Chem, 16 (19): (8781-94). [PMID:18790648]
7. Bonnard E, Poras H, Nadal X, Maldonado R, Fournié-Zaluski MC, Roques BP. (2015) Long-lasting oral analgesic effects of N-protected aminophosphinic dual ENKephalinase inhibitors (DENKIs) in peripherally controlled pain.. Pharmacol Res Perspect, 3 (2): (e00116). [PMID:25692029]
8. Chen H, Noble F, Roques BP, Fournié-Zaluski MC. (2001) Long lasting antinociceptive properties of enkephalin degrading enzyme (NEP and APN) inhibitor prodrugs.. J Med Chem, 44 (21): (3523-30). [PMID:11585456]
9. Pinto M, Rougeot C, Gracia L, Rosa M, García A, Arsequell G, Valencia G, Centeno NB. (2012) Proposed Bioactive Conformations of Opiorphin, an Endogenous Dual APN/NEP Inhibitor.. ACS Med Chem Lett, 3 (1): (20-4). [PMID:24900367]
10. David C Pryde, Graham N Maw, Simon Planken, Michelle Y Platts, Vivienne Sanderson, Martin Corless, Alan Stobie, Christopher G Barber, Rachel Russell, Laura Foster, Laura Barker, Christopher Wayman, Piet Van Der Graaf, Peter Stacey, Debbie Morren, Christopher Kohl, Kevin Beaumont, Sara Coggon, Michael Tute,. (2006-07-11) Novel selective inhibitors of neutral endopeptidase for the treatment of female sexual arousal disorder. Synthesis and activity of functionalized glutaramides.. Journal of medicinal chemistry, 49 ((14)): ( 4409-4424 ). [PMID:16821800]
11. Berger Y, Dehmlow H, Blum-Kaelin D, Kitas EA, Löffler BM, Aebi JD, Juillerat-Jeanneret L.. (2005) Endothelin-converting enzyme-1 inhibition and growth of human glioblastoma cells.. J Med Chem, 48 (2): (483-498). [PMID:15658862] [10.1021/jm040857x]
12. Deaton DN, Graham KP, Gross JW, Miller AB.. (2008) Thiol-based angiotensin-converting enzyme 2 inhibitors: P1' modifications for the exploration of the S1' subsite.. Bioorg Med Chem Lett, 18 (5): (1681-1687). [PMID:18243695] [10.1016/j.bmcl.2008.01.046]
13. Abdul-Hay SO, Lane AL, Caulfield TR, Claussin C, Bertrand J, Masson A, Choudhry S, Fauq AH, Maharvi GM, Leissring MA.. (2013) Optimization of peptide hydroxamate inhibitors of insulin-degrading enzyme reveals marked substrate-selectivity.. J Med Chem, 56 (6): (2246-2255). [PMID:23437776] [10.1021/jm301280p]
14. Charton J, Gauriot M, Guo Q, Hennuyer N, Marechal X, Dumont J, Hamdane M, Pottiez V, Landry V, Sperandio O, Flipo M, Buee L, Staels B, Leroux F, Tang WJ, Deprez B, Deprez-Poulain R.. (2014) Imidazole-derived 2-[N-carbamoylmethyl-alkylamino]acetic acids, substrate-dependent modulators of insulin-degrading enzyme in amyloid-β hydrolysis.. Eur J Med Chem, 79 (184-193). [PMID:24735644] [10.1016/j.ejmech.2014.04.009]
15. Choi JY, Fuerst R, Knapinska AM, Taylor AB, Smith L, Cao X, Hart PJ, Fields GB, Roush WR.. (2017) Structure-Based Design and Synthesis of Potent and Selective Matrix Metalloproteinase 13 Inhibitors.. J Med Chem, 60 (13): (5816-5825). [PMID:28653849] [10.1021/acs.jmedchem.7b00514]
16. Sattigeri JA, Sethi S, Davis JA, Ahmed S, Rayasam GV, Jadhav BG, Chilla SM, Datta D, Gadhave A, Tulasi VK, Jain T, Voleti S, Benjamin B, Udupa S, Jain G, Singh Y, Srinivas K, Bansal VS, Ray A, Bhatnagar PK, Cliffe IA.. (2017) Approaches towards the development of chimeric DPP4/ACE inhibitors for treating metabolic syndrome.. Bioorg Med Chem Lett, 27 (11): (2313-2318). [PMID:28442252] [10.1016/j.bmcl.2017.04.036]
17. McKinnie SMK, Wang W, Fischer C, McDonald T, Kalin KR, Iturrioz X, Llorens-Cortes C, Oudit GY, Vederas JC.. (2017) Synthetic Modification within the "RPRL" Region of Apelin Peptides: Impact on Cardiovascular Activity and Stability to Neprilysin and Plasma Degradation.. J Med Chem, 60 (14): (6408-6427). [PMID:28685579] [10.1021/acs.jmedchem.7b00723]
18. McKinnell RM, Fatheree P, Choi SK, Gendron R, Jendza K, Olson Blair B, Budman J, Hill CM, Hegde LG, Yu C, McConn D, Hegde SS, Marquess DG, Klein U.. (2019) Discovery of TD-0212, an Orally Active Dual Pharmacology AT₁ Antagonist and Neprilysin Inhibitor (ARNI).. ACS Med Chem Lett, 10 (1): (86-91). [PMID:30655952] [10.1021/acsmedchemlett.8b00462]
19. Kawanami T, Karki RG, Cody E, Liu Q, Liang G, Ksander GM, Rigel DF, Schiering N, Gong Y, Coppola GM, Iwaki Y, Sun R, Neubert A, Fan L, Ingles S, D'Arcy A, Villard F, Ramage P, Jeng AY, Leung-Chu J, Liu J, Beil M, Fu F, Chen W, Cumin F, Wiesmann C, Mogi M.. (2020) Structure-Guided Design of Substituted Biphenyl Butanoic Acid Derivatives as Neprilysin Inhibitors.. ACS Med Chem Lett, 11 (2): (188-194). [PMID:32071687] [10.1021/acsmedchemlett.9b00578]
20. Sharma U, Cozier GE, Sturrock ED, Acharya KR.. (2020) Molecular Basis for Omapatrilat and Sampatrilat Binding to Neprilysin-Implications for Dual Inhibitor Design with Angiotensin-Converting Enzyme.. J Med Chem, 63 (10): (5488-5500). [PMID:32337993] [10.1021/acs.jmedchem.0c00441]
21. O'Connell, J E JE, Jardine, A G AG, Davidson, G G and Connell, J M JM.. (1992) Candoxatril, an orally active neutral endopeptidase inhibitor, raises plasma atrial natriuretic factor and is natriuretic in essential hypertension.. Journal of hypertension, [PMID:1315825]
22. Elsner, D D, Müntze, A A, Kromer, E P EP and Riegger, G A GA.. (1992) Effectiveness of endopeptidase inhibition (candoxatril) in congestive heart failure.. The American journal of cardiology, (15): [PMID:1386491]
23. D'Adamio, L L, Shipp, M A MA, Masteller, E L EL and Reinherz, E L EL.. (1989) Organization of the gene encoding common acute lymphoblastic leukemia antigen (neutral endopeptidase 24.11): multiple miniexons and separate 5' untranslated regions.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2528730]
24. Yandle, T G TG, Brennan, S O SO, Espiner, E A EA, Nicholls, M G MG and Richards, A M AM.. Endopeptidase-24.11 in human plasma degrades atrial natriuretic factor (ANF) to ANF(99-105/106-126).. Peptides, [PMID:2531377]
25. Shipp, M A MA and 7 more authors.. (1988) Molecular cloning of the common acute lymphoblastic leukemia antigen (CALLA) identifies a type II integral membrane protein.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2968607]
26. Letarte, M M and 7 more authors.. (1988) Common acute lymphocytic leukemia antigen is identical to neutral endopeptidase.. The Journal of experimental medicine, (1): [PMID:2971756]
27. Vanneste, Y Y, Michel, A A, Dimaline, R R, Najdovski, T T and Deschodt-Lanckman, M M.. (1988) Hydrolysis of alpha-human atrial natriuretic peptide in vitro by human kidney membranes and purified endopeptidase-24.11. Evidence for a novel cleavage site.. The Biochemical journal, (1): [PMID:2972276]
28. Malfroy, B B, Kuang, W J WJ, Seeburg, P H PH, Mason, A J AJ and Schofield, P R PR.. (1988) Molecular cloning and amino acid sequence of human enkephalinase (neutral endopeptidase).. FEBS letters, (29): [PMID:3162217]
29. Skidgel, R A RA, Engelbrecht, S S, Johnson, A R AR and Erdös, E G EG.. Hydrolysis of substance p and neurotensin by converting enzyme and neutral endopeptidase.. Peptides, [PMID:6208535]
30. Hirata, Y Y and 6 more authors.. (1994) Mechanisms of the natriuretic effects of neutral endopeptidase inhibition in Dahl salt-sensitive and salt-resistant rats.. Journal of cardiovascular pharmacology, [PMID:7511759]
31. Le Moual, H H, Dion, N N, Roques, B P BP, Crine, P P and Boileau, G G.. (1994) Asp650 is crucial for catalytic activity of neutral endopeptidase 24-11.. European journal of biochemistry, (1): [PMID:8168535]
32. Oefner, C C, D'Arcy, A A, Hennig, M M, Winkler, F K FK and Dale, G E GE.. (2000) Structure of human neutral endopeptidase (Neprilysin) complexed with phosphoramidon.. Journal of molecular biology, (18): [PMID:10669592]
33. Intengan, H D HD and Schiffrin, E L EL.. (2000) Vasopeptidase inhibition has potent effects on blood pressure and resistance arteries in stroke-prone spontaneously hypertensive rats.. Hypertension (Dallas, Tex. : 1979), [PMID:10856267]
34. Chen, X X, Ji, Z L ZL and Chen, Y Z YZ.. (2002) TTD: Therapeutic Target Database.. Nucleic acids research, (1): [PMID:11752352]
35. Zhang, Hui H, Li, Xiao-Jun XJ, Martin, Daniel B DB and Aebersold, Ruedi R.. (2003) Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry.. Nature biotechnology, [PMID:12754519]
36. Oefner, Christian C, Roques, Bernard P BP, Fournie-Zaluski, Marie-Claude MC and Dale, Glenn E GE.. (2004) Structural analysis of neprilysin with various specific and potent inhibitors.. Acta crystallographica. Section D, Biological crystallography, [PMID:14747736]
37. Rice, Gillian I GI, Thomas, Daniel A DA, Grant, Peter J PJ, Turner, Anthony J AJ and Hooper, Nigel M NM.. (2004) Evaluation of angiotensin-converting enzyme (ACE), its homologue ACE2 and neprilysin in angiotensin peptide metabolism.. The Biochemical journal, (1): [PMID:15283675]
38. Sansoè, Giovanni G and 9 more authors.. (2005) Neutral endopeptidase (EC 3.4.24.11) in cirrhotic liver: a new target to treat portal hypertension?. Journal of hepatology, [PMID:16085334]
39. Sansoè, G G and 9 more authors.. (2006) Overexpression of kidney neutral endopeptidase (EC 3.4.24.11) and renal function in experimental cirrhosis.. American journal of physiology. Renal physiology, [PMID:16449355]
40. Wisner, Anne A and 6 more authors.. (2006) Human Opiorphin, a natural antinociceptive modulator of opioid-dependent pathways.. Proceedings of the National Academy of Sciences of the United States of America, (21): [PMID:17101991]
41. Oefner, Christian C, Pierau, Sabine S, Schulz, Henk H and Dale, Glenn E GE.. (2007) Structural studies of a bifunctional inhibitor of neprilysin and DPP-IV.. Acta crystallographica. Section D, Biological crystallography, [PMID:17704566]
42. Zheng, Rong R and 6 more authors.. (2010) Neutral endopeptidase is a myristoylated protein.. Molecular and cellular biochemistry, [PMID:19756956]
43. Morisaki, Naoko N and 5 more authors.. (2010) Neprilysin is identical to skin fibroblast elastase: its role in skin aging and UV responses.. The Journal of biological chemistry, (17): [PMID:20876573]
44. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569]
45. Smith, A Ian AI and 12 more authors.. (2016) N-terminal domain of Bothrops asper Myotoxin II Enhances the Activity of Endothelin Converting Enzyme-1 and Neprilysin.. Scientific reports, (2): [PMID:26931059]
46. Higuchi, Yujiro Y and 32 more authors.. (2016) Mutations in MME cause an autosomal-recessive Charcot-Marie-Tooth disease type 2.. Annals of neurology, [PMID:26991897]
47. Depondt, Chantal C and 6 more authors.. (2016) MME mutation in dominant spinocerebellar ataxia with neuropathy (SCA43).. Neurology. Genetics, [PMID:27583304]
48. Auer-Grumbach, Michaela M and 31 more authors.. (2016) Rare Variants in MME, Encoding Metalloprotease Neprilysin, Are Linked to Late-Onset Autosomal-Dominant Axonal Polyneuropathies.. American journal of human genetics, (1): [PMID:27588448]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

CD10 Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器