ALDH6A1 Mouse mAb

COA

级别和纯度:

无载体

已验证

ExactAb™

0.5 mg/mL

应用:

IF/ICC
IHC
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human), 小鼠(Mouse)
亚型: Mouse IgG1
克隆号: E7S8
偶联: Unconjugated

有货

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货号 (SKU)	包装规格	是否现货
Ab088099-10μl	10μl	现货
Ab088099-50μl	50μL	现货
Ab088099-100μl	100μL	期货
Ab088099-1ml	1ml	期货

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Accession#: Q02252 (1) ALDH6A1 (1) Gene ID: 4329 (1)

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基本信息

产品名称	ALDH6A1 Mouse mAb
别名	醛脱氢酶 6 家族成员 A1抗体 \| ALDH6A1 小鼠单克隆抗体 \| ALDH6A1 小鼠单抗
英文别名	AI314632 antibody \| Aldehyde dehydrogenase 6 family member A1 antibody \| Aldehyde dehydrogenase family 6 member A1 antibody \| Aldehyde dehydrogenase family 6, subfamily A1 antibody \| ALDH6A1 antibody \| Malonate semialdehyde dehydrogenase antibody \| Malona
规格或纯度	ExactAb™, Validated, Carrier Free, 0.5 mg/mL
生化机理	在缬氨酸和嘧啶代谢中发挥作用。结合脂肪酰基-CoA。
宿主种属	小鼠(Mouse)
特异性	ALDH6A1
种属反应性	人(Human),小鼠(Mouse)
免疫原	Recombinant fusion protein of human ALDH6A1.
阳性对照	WB: Mouse liver, LNCaP lysates. IHC: Human colon tissue. IF/ICC: MCF7 cells.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	E7S8
Format	Whole IgG
亚型	Mouse IgG1
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, 30% Glycerol, 0.01% Sodium azide, pH7.3
防腐剂	0.01% Sodium azide
浓度	0.5 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4℃ 短期储存（1-2 周）。-20℃长期保存（12 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

图片

Western Blot: ALDH6A1 Mouse mAb (Ab088099)

ALDH6A1 Mouse mAb (Ab088099) - Western Blot
All lanes: ALDH6A1 Mouse mAb (Ab088099) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution

Predicted band size: 57 kDa
Observed band size: 54 kDa
Exposure time: 28.1 s

Western Blot: ALDH6A1 Mouse mAb (Ab088099)

IF/ICC: ALDH6A1 Mouse mAb (Ab088099)

ALDH6A1 Mouse mAb (Ab088099) - IF/ICC
Immunofluorescent analysis of ALDH6A1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ALDH6A1 Mouse mAb (Ab088099) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

IHC: ALDH6A1 Mouse mAb (Ab088099)

ALDH6A1 Mouse mAb (Ab088099) - IHC
Immunohistochemical analysis of ALDH6A1 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ALDH6A1 Mouse mAb (Ab088099) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

应用