Recombinant SIRP alpha Antibody - Primary antibody, specific to SIRPA, Rabbit IgG
Synonyms
Signal regulatory protein alpha type 1 antibody | Bit antibody | Brain Ig like molecule with tyrosine based activation motifs antibody | Brain Ig-like molecule with tyrosine-based activation motifs antibody | Brain immunoglobulin like molecule with tyrosi
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.7 mg/mL
Host species
Rabbit
Specificity
SIRPA
Immunogen
Recombinant protein of human SIRP alpha (AA 31-373)
Positive Control
WB: HEK293, A549, U937 and THP-1 cell lysates. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Note
本产品即将售完下架，替代产品为 Ab127637
Product Description
Rabbit anti Human SIRP alpha Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Shape
Liquid
Concentration
0.7 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Associated Targets(Human)
SIRPATbioTyrosine-protein phosphatase non-receptor type substrate 1 (0 Activities)
Recombinant SIRP alpha Antibody (Ab127634) - Western Blot All lanes: Recombinant SIRP alpha Antibody (Ab127634) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa Observed band size: 74 kDa Exposure time: 65.4 s
Recombinant SIRP alpha Antibody (Ab127634) - Western Blot All lanes: Recombinant SIRP alpha Antibody (Ab127634) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa Observed band size: 77 kDa Exposure time: 30.0 s
Recombinant SIRP alpha Antibody (Ab127634) - IF Immunocytochemistry analysis of SIRP alpha (green) in HeLa using Recombinant SIRP alpha Antibody (Ab127634), and DAPI (blue).
Application
Application
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Find and download the COA for your product by matching the lot number on the packaging.
ZJ24F0403986