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Recombinant PKR Antibody - Primary antibody, specific to PKR, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab122177
Grouped product items
SKU Size
Availability
 Price Qty
Ab122177-10μl
10μl
2
$69.90
Ab122177-50μl
50μl
1
$199.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human PKR Antibody; WB, IHC, ICC, IF; Unconjugated

View related series
Click to view target information: EIF2AK2
Accession#:P19525 (2) EIF2AK2 (2) Gene ID:5610 (2) PKR (2)

Basic Description

Product Name Recombinant PKR Antibody - Primary antibody, specific to PKR, Rabbit IgG
Synonyms 2.7.10.2 | EC 2.7.11.1 | eIF-2A protein kinase 2 | Eukaryotic translation initiation factor 2-alpha kinase 2 | Interferon-inducible RNA-dependent protein kinase | P1/eIF-2A protein kinase | p68 kinase | PKR | Protein kinase R | Protein kinase RNA-activate
Specifications & Purity ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species Rabbit
Specificity PKR
Immunogen A synthetic peptide derived from human PKR (AA 508-526)
Positive Control WB: K562, Hep G2 and HeLa cell lysates. IHC: Human cholangiocarcinoma tissue. ICC/IF: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human PKR Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function：
Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Antigen affinity purified
Shape Liquid
Concentration 0.2 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

EIF2AK2 Tchem Interferon-induced, double-stranded RNA-activated protein kinase (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant PKR Antibody (Ab122177) - Western Blot
All lanes: Recombinant PKR Antibody (Ab122177) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 62 kDa
Observed band size: 67 kDa
Exposure time: 22.9 s

Recombinant PKR Antibody (Ab122177) - IF/ICC
Immunocytochemistry analysis of PKR (green) in HeLa using Recombinant PKR Antibody (Ab122177), and DAPI (blue).

Recombinant PKR Antibody (Ab122177) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant PKR Antibody (Ab122177). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
WB

1/500-1/1000
IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0506153 Certificate of Analysis May 27, 2024 Ab122177
ZJ24F0506152 Certificate of Analysis May 27, 2024 Ab122177

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