This is a demo store. No orders will be fulfilled.

Recombinant MLH1 Antibody - Primary antibody, specific to MLH1, Rabbit IgG

    Application:
  • IF/ICC
  • WB
  • Host species: Rabbit
  • Reactive species: Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab115549
Grouped product items
SKU Size
Availability
Price Qty
Ab115549-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$66.90
Ab115549-50μl
50μl
1
$199.90
Ab115549-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab115549-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

Recombinant; Rabbit anti Human MLH1 Antibody; WB, ICC, IF; Unconjugated

Basic Description

Product Name Recombinant MLH1 Antibody - Primary antibody, specific to MLH1, Rabbit IgG
Synonyms COCA 2 antibody | COCA2 antibody | DNA mismatch repair protein Mlh1 antibody | FCC 2 antibody | FCC2 antibody | hMLH 1 antibody | hMLH1 antibody | HNPCC 2 antibody | HNPCC antibody | HNPCC2 antibody | MGC5172antibody | MLH 1 antibody | MLH1 antibody | MLH
Specifications & Purity ExactAb™, Validated, Recombinant, 0.6 mg/mL
Host species Rabbit
Specificity MLH1
Immunogen Recombinant protein of human MLH1 (AA 350-756)
Positive Control WB: HeLa, A549, Jurkat, A431, HEK293, Hep G2, MCF7, K562 and MOLT-4 cell lysates. ICC/IF: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human MLH1 Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function
Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Antigen affinity purified
Shape Liquid
Concentration 0.6 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

MLH1 Tbio DNA mismatch repair protein Mlh1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant MLH1 Antibody (Ab115549) - Western Blot
All lanes: Recombinant MLH1 Antibody (Ab115549) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 200.0 s


Recombinant MLH1 Antibody (Ab115549) - IF/ICC
Immunocytochemistry analysis of MLH1 (green) in HeLa using Recombinant MLH1 Antibody (Ab115549), and cells were counterstained with DAPI (blue).


Application

Application Dilution info
WB

1/500-1/1000

IF/ICC

1/50-1/200

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1202153 Certificate of Analysis Dec 20, 2023 Ab115549
ZJ23F1202154 Certificate of Analysis Dec 20, 2023 Ab115549

Solution Calculators

Reviews

Customer Reviews

Shall we send you a message when we have discounts available?

Remind me later

Thank you! Please check your email inbox to confirm.

Oops! Notifications are disabled.