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Recombinant HDAC1 Antibody - Primary antibody, specific to HDAC1, Rabbit IgG

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Cow,Green Monkey,Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Clone Number: RR672
  • Conjugation: Unconjugated
In stock
Item Number
Ab107012
Grouped product items
SKU Size
Availability
 Price Qty
Ab107012-10μl
10μl
5
$59.90
Ab107012-50μl
50μl
1
$177.90
Ab107012-100μl
100μl
1
$267.90
Ab107012-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,007.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant (RR672); Rabbit anti Human HDAC1 Antibody; WB, IHC, Flow, IF, ICC; Unconjugated

View related series
Click to view target information: HDAC1
Accession#:Q13547 (3) Flow Cytometry (1158) Gene ID:3065 (3) HDAC1 (3)

Basic Description

Product Name Recombinant HDAC1 Antibody - Primary antibody, specific to HDAC1, Rabbit IgG
Synonyms DKFZp686H12203 antibody | GON 10 antibody | HD1 antibody | HDAC 1 antibody | HDAC1 antibody | HDAC1_HUMAN antibody | Histone deacetylase 1 antibody | Reduced potassium dependency yeast homolog like 1 antibody | RPD3 antibody | RPD3L1 antibody | HDAC1 | GO
Specifications & Purity ExactAb™, Validated, Recombinant, High performance, 0.125 mg/mL
Host species Rabbit
Specificity HDAC1
Immunogen A synthetic peptide corresponding to the C-term of HDAC1 was used as an immunogen.
Positive Control WB: Jurkat, K562, MOLT-4, HeLa, THP-1, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human colon tissue. Flow: HeLa cells. IF/ICC: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, High Performance, Recombinant, Validated
Product Description

Rabbit anti Human HDAC1 Antibody, Recombinant (RR672), could be used for WB, IHC, Flow, IF, ICC and so on.

Application: 

WB: 1/1000 - 1/2000       

IHC: 1/100 - 1/200

Flow: 1/50 - 1/200

IF/ICC: 1/200 - 1/500

Protein Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.

Post-translational

Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.

Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.

Ubiquitinated by CHFR, leading to its degradation by the proteasome.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 0.125 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

HDAC1 Tclin Histone deacetylase 1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
HDAC1 Tclin Histone deacetylase 1 (10854 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
HDAC1 Tclin Histone deacetylase (6747 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
HDAC1 Tclin Histone deacetylase (HDAC1 and HDAC2) (618 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
HDAC1 Tclin Histone deacetylase 1/3/5/8 (49 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
HDAC1 Tclin Histone deacetylase 1/2/3/6 (89 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
HDAC1 Tclin Histone deacetylase 1/2/3 (160 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
HDAC1 Tclin Histone deacetylase 1/8 (2 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
HDAC1 Tclin Histone deacetylase 1/Nuclear receptor corepressor 2 (6 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
HDAC1 Tclin BRD4/HDAC1 (6 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
HDAC1 Tclin Histone deacetylase 1/6 (127 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
HDAC1 Tclin HDAC1/REST corepressor 3 (5 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
HDAC1 Tclin Cereblon/Histone deacetylase 1 (11 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
HDAC1 Tclin Class 1 histone deacetylase (459 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant HDAC1 Antibody (Ab107012) - Western Blot
All lanes: Recombinant HDAC1 Antibody (Ab107012) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 55 kDa
Observed band size: 61 kDa
Exposure time: 10.0 s

Recombinant HDAC1 Antibody (Ab107012) - Western Blot
All lanes: Recombinant HDAC1 Antibody (Ab107012) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 55 kDa
Observed band size: 61 kDa
Exposure time: 19.5 s

Recombinant HDAC1 Antibody (Ab107012) - IF/ICC
IF analysis of HDAC1 (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant HDAC1 Antibody (Ab107012) at 0.25 μg/mL in blocking buffer overnight at 4 °C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Recombinant HDAC1 Antibody (Ab107012) - IF/ICC
Recombinant HDAC1 Antibody (Ab107012) staining HDAC1 in Hela cells by IF/ICC (immunofluorescence/immunocytochemistry). Cells were
fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant HDAC1 Antibody (Ab107012) (1/500) at 4°C. An AF594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An AF594-conjugated Goat Anti-Rabbit IgG (1/500).

Recombinant HDAC1 Antibody (Ab107012) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human colon tissue labelling HDAC1 with Recombinant HDAC1 Antibody (Ab107012) at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Recombinant HDAC1 Antibody (Ab107012) - Flow Cytometry 
Overlay histogram showing HeLa cells stained with Recombinant HDAC1 Antibody (Ab107012) (Red).  The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant HDAC1 Antibody (Ab107012) (1/200 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L)  at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

Application

Application Dilution info
IHC

1/100 - 1/200
WB

1/1000 - 1/2000
Flow Cytometry

1/50 - 1/200
IF/ICC

1/200 - 1/500

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1001410 Certificate of Analysis Oct 26, 2023 Ab107012
ZJ23F1001411 Certificate of Analysis Oct 26, 2023 Ab107012

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