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Recombinant ERK1 Antibody - Primary antibody, for WB,Flow,IF,ICC,IHC,IP various applications, from Human, generated in Rabbit, specific to MAPK3, Rabbit IgG

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • IHC
  • IP
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Clone Number: RR671
  • Conjugation: Unconjugated
In stock
Item Number
Ab102223
Grouped product items
SKU Size
Availability
 Price Qty
Ab102223-10μl
10μl
4
$69.90
Ab102223-50μl
50μl
1
$207.90
Ab102223-100μl
100μl
1
$297.90
Ab102223-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,517.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant (RR671); Rabbit anti Human ERK1 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated

View related series
Click to view target information: MAPK3
Accession#:P27361 (2) ERK1 (2) Flow Cytometry (1158) Gene ID:5595 (2) MAPK3 (2)

Basic Description

Product Name Recombinant ERK1 Antibody - Primary antibody, for WB,Flow,IF,ICC,IHC,IP various applications, from Human, generated in Rabbit, specific to MAPK3, Rabbit IgG
Synonyms ERK 1 antibody | ERK-1 antibody | ERK1 antibody | ERT 2 antibody | ERT2 antibody | Extracellular Signal Regulated Kinase 1 antibody | Extracellular signal related kinase 1 antibody | Extracellular signal-regulated kinase 1 antibody | HGNC6877 antibody | H
Specifications & Purity ExactAb™, Validated, Recombinant, High performance, 2mg/mL
Biochemical and Physiological Mechanisms unction Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translati
Host Species Rabbit
Host species Rabbit
Specificity MAPK3
Application WB,Flow,IF,ICC,IHC,IP
Immunogen A synthetic peptide corresponding to the N-term of ERK1 was used as an immunogen.
Positive Control WB: HeLa, A431, NIH/3T3, Jurkat and K562 cell lysates. IHC: Cervix cancer tissue. Flow: HeLa cells. ICC/IF: HeLa cells. IP: A375 whole cell lysate.
Conjugation Unconjugated
Grade ExactAb™, High Performance, Recombinant, Validated
Product Description

Rabbit anti Human ERK1 Antibody, Primary antibody, could be used for WB, IHC, Flow, IF/ICC and so on.

Product Properties

Antibody Type Primary antibody
Clonality Recombinant
Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Protein A purified
Source CHO supernatant
Shape Liquid
Concentration 2mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C for 2 years. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

MAPK3 Tchem Mitogen-activated protein kinase 3 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
MAPK3 Tchem MAP kinase ERK1 (4725 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
MAPK3 Tchem Mitogen-activated protein kinase; ERK1/ERK2 (651 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant ERK1 Antibody (Ab102223) - Western Blot
All lanes: Recombinant ERK1 Antibody (Ab102223) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 43 kDa
Observed band size: 40 kDa
Exposure time: 27.0 s

Recombinant ERK1 Antibody (Ab102223) - IF/ICC
Recombinant ERK1 Antibody (Ab102223) staining ERK1 in HeLa cells by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant ERK1 Antibody (Ab102223) (1/50) at 4°C. An AF488-conjugatedGoat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.

Control: PBS and secondary antibody, An AF488-conjugatedGoat Anti-Rabbit IgG (1/500).

Recombinant ERK1 Antibody (Ab102223) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant ERK1 Antibody (Ab102223) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant ERK1 Antibody (Ab102223) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.

Recombinant ERK1 Antibody (Ab102223) - IP
ERK1 was immunoprecipitated from 0.4 mg of A375 whole cell lysate with Recombinant ERK1 Antibody (Ab102223) at 1/50 dilution
2nd Ab: GAR HRP for IP 1/500

Lane 1: Recombinant ERK1 Antibody (Ab102223) IP in A375 whole cell lysate
Lane 2: PBS instead of Recombinant ERK1 Antibody (Ab102223) in A375 whole cell lysate
Lane 3: A375 whole cell lysate, 10 μg (input)

Exposure: 120.0 s.

Recombinant ERK1 Antibody (Ab102223) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded
sections) analysis Cervix cancer tissue labelling ERK1 with Recombinant ERK1 Antibody (Ab102223) at 1:200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Application

Application Dilution info
IHC

1/100 - 1/200
IF/ICC

1/50 - 1/200
IP

1/50

Flow Cytometry

1/200 - 1/1000

WB

1/1000 - 1/10000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0404288 Certificate of Analysis Apr 09, 2024 Ab102223
ZJ23F0901102 Certificate of Analysis Sep 18, 2023 Ab102223
ZJ23F0901103 Certificate of Analysis Sep 18, 2023 Ab102223

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Documents & Articles

Flow Cytometry
Categories: Technical articles
Tags:
Flow Cytometry
Flow cytometry analysis
Categories: Technical articles
Tags:
Forward scatter Side scatter Dichroic filters/mirrors The multicolor flow cytometer
How to choose and use antibodies-primary antibody?
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Antibody applications and techniques
Categories: Technical articles
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ICC ELISA WB IHC
Flow cytometry protocol
Categories: Protocols
Tags:
Antibodies Flow Cytometry Flow Antibodies Reactive dyes Indirect antibody labelling
Immunoprecipitation (IP) lysates and reagents
Categories: Protocols
Tags:
Immunoprecipitation lysate Tissue lysate
Immunohistochemical staining protocol for frozen samples (IHC-Fr)
Categories: Protocols
Tags:
IHC Monoclonal antibodies Immunohistochemistry Frozen section Recombinant Antibodies
Immunohistochemistry (IHC) staining antigen retrieval protocol
Categories: Protocols
Tags:
IHC Monoclonal antibodies Immunohistochemistry Recombinant Antibodies Antigen Retrieval
General Immunoprecipitation Protocol
Categories: Protocols
Tags:
Immunoprecipitation Western blot Cell lysate Co-immunoprecipitation IP Protein interaction Protein A/G magnetic beads
Immunofluorescence Staining Protocol (Methanol permeabilization)
Categories: Protocols
Tags:
Frozen section Immunofluorescence staining Tissue section Methanol permeability IF
RNA Immunoprecipitation (RIP) Protocol
Categories: Protocols
Tags:
IP Protein A/G magnetic beads RNA immunoprecipitation RIP RNA protein interaction Isotype control antibody Input Crosslinking
Immobilized IP Protocol
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Tags:
Immunoprecipitation WB Western blot IP Protein interaction Immobilization immunoprecipitation Immobilized antibody
Flow Cytometry Using Methanol Permeabilization
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Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

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