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Recombinant ACTA2 Antibody - Primary antibody, specific to ACTA2, Rabbit IgG

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Cow,Dog,Green Monkey,Human,Mouse,Pig,Rat,Zebrafish
  • Isotype: Rabbit IgG
  • Clone Number: RR645
  • Conjugation: Unconjugated
In stock
Item Number
Ab088446
Grouped product items
SKU Size
Availability
 Price Qty
Ab088446-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab088446-50μl
50μl
1
$207.90
Ab088446-100μl
100μl
2
$297.90
Ab088446-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,187.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant (RR645); Rabbit anti Human ACTA2 Antibody; WB, IHC, Flow, IF, ICC; Unconjugated

View related series
Click to view target information: ACTA2
Accession#:P62736 (7) ACTA2 (8) alpha-Smooth Muscle Actin (3) Flow Cytometry (1158) Gene ID:59 (5) Loading control (34)

Basic Description

Product Name Recombinant ACTA2 Antibody - Primary antibody, specific to ACTA2, Rabbit IgG
Synonyms a actin antibody | AAT6 antibody | ACTA_HUMAN antibody | ACTA2 antibody | Actin alpha 2 smooth muscle aorta antibody | Actin aortic smooth muscle antibody | Actin, aortic smooth muscle antibody | ACTSA antibody | ACTVS antibody | Alpha 2 actin antibody |
Specifications & Purity ExactAb™, Validated, Recombinant, High performance, 0.05 mg/mL
Host species Rabbit
Specificity ACTA2
Immunogen A synthetic peptide corresponding to residues on the N-terminus of human Alpha Actin 2 was used as an immunogen.
Positive Control WB: Hela, HEK293, LNCap, Raji, Hep G2, A431, Jurkat, HUVEC, C6, NIH/3T3 cell lyeates. IHC: Placenta. Flow: HeLa cells. IF/ICC: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, High Performance, Recombinant, Validated
Product Description

Rabbit anti Human ACTA2 Antibody, Recombinant (RR645), could be used for WB, IHC, Flow, IF, ICC and so on.
Application:
WB: 1/1000 - 1/2000
IHC: 1/2000 - 1/4000
Flow: 1/20 - 1/100
IF/ICC: 1/80 - 1/250

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 0.05 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

ACTA2 Tbio Actin, aortic smooth muscle (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant ACTA2 Antibody (Ab088446) - Western Blot
All lanes: Recombinant ACTA2 Antibody (Ab088446) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 42 kDa
Observed band size: 44 kDa
Exposure time: 80.0 s

Recombinant ACTA2 Antibody (Ab088446) - IF
IF analysis of ACTA2 (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant ACTA2 Antibody (Ab088446) at 1/100 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Recombinant ACTA2 Antibody (Ab088446) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of placenta tissue labelling ACTA2 with Recombinant ACTA2 Antibody (Ab088446) at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Recombinant ACTA2 Antibody (Ab088446) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant ACTA2 Antibody (Ab088446) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant ACTA2 Antibody (Ab088446) (1/100 dilution) in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at 4°C. Unlabeled sample (Green) was used as a control.

Application

Application Dilution info
WB

1/1000 - 1/2000
IF/ICC

1/80 - 1/250
Flow Cytometry

1/20 - 1/100

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0901100 Certificate of Analysis Sep 18, 2023 Ab088446
ZJ23F0901101 Certificate of Analysis Sep 18, 2023 Ab088446

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