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alpha-Smooth Muscle Actin Mouse mAb - Primary antibody, specific to Alpha smooth muscle Actin, Mouse IgG1

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alpha-Smooth Muscle Actin Mouse mAb - Primary antibody, specific to Alpha smooth muscle Actin, Mouse IgG1

Grade & Purity:

High Performance

See COA

Application:

IHC
WB

Host species: Mouse
Reactive species: Cow,Dog,Human,Mouse,Pig,Rat
Isotype: Mouse IgG1
Clone Number: F1
Conjugation: Unconjugated

In stock

Item Number

Ab088443

Grouped product items
SKU	Size	Availability	Price	Qty
Ab088443-10μl	10μl	4	$59.90
Ab088443-50μl	50μl	3	$177.90
Ab088443-100μl	100μl	2	$297.90
Ab088443-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,007.90

mAb (F1); Mouse anti Human alpha-Smooth Muscle Actin Antibody; WB, IHC; Unconjugated

View related series

Click to view target information: ACTA2

Accession#:P62736 (7) ACTA2 (8) alpha-Smooth Muscle Actin (3) Gene ID:59 (5) Loading control (34)

Basic Description

Product Name	alpha-Smooth Muscle Actin Mouse mAb - Primary antibody, specific to Alpha smooth muscle Actin, Mouse IgG1
Synonyms	A actin antibody \| AAT6 antibody \| ACTA_HUMAN antibody \| ACTA2 antibody \| Actin alpha 2 smooth muscle aorta antibody \| Actin aortic smooth muscle antibody \| Actin, aortic smooth muscle antibody \| ACTSA antibody \| ACTVS antibody \| Alpha 2 actin antibody \|
Specifications & Purity	Carrier Free, ExactAb™, Validated, High Performance, See COA
Host species	Mouse
Specificity	Alpha smooth muscle Actin
Immunogen	Recombinant ACTA2 expressed in E.coli (AA 167-377).
Positive Control	WB: NIH/3T3, Mouse skeletal and Mouse heart lysates; IHC: Human Ovarian cancer tissues, Human Rectum tissue, Rat Skeletal muscle tissues, Rat Lung tissue, Mouse Pancreas tissues, Mouse Spleen tissues.
Conjugation	Unconjugated
Grade	Carrier Free, ExactAb™, High Performance, Validated
Product Description	Mouse anti Human Alpha smooth muscle Actin Antibody, Monoclonal (F1), could be used for WB, IHC and so on. Application WB:0.001-2 μg/mL IHC: 5-20 µg/mL Protein Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Product Properties

Isotype	Mouse IgG1
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A + Protein G purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

ACTA2 Tbio Actin, aortic smooth muscle (0 Activities)

Discover Target: ACTA2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: Alpha smooth muscle Actin Mouse mAb (Ab088443)

Alpha smooth muscle Actin Mouse mAb (Ab088443) - Western Blot
All lanes: Alpha smooth muscle Actin Mouse mAb (Ab088443) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5.6 s

Western Blot: Alpha smooth muscle Actin Mouse mAb (Ab088443)

Alpha smooth muscle Actin Mouse mAb (Ab088443) - Western Blot
All lanes: Alpha smooth muscle Actin Mouse mAb (Ab088443) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 26.7 s

IHC: Alpha smooth muscle Actin Mouse mAb (Ab088443)

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Human Ovarian cancer Tissue
Primary Ab:10ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse
IgG Polyclonal Antibody.

IHC: Alpha smooth muscle Actin Mouse mAb (Ab088443)

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Human Rectum Tissue
Primary Ab:10ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Alpha smooth muscle Actin Mouse mAb (Ab088443)

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Rat Skeletal muscle Tissue
Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Alpha smooth muscle Actin Mouse mAb (Ab088443)

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Rat Lung Tissue
Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Alpha smooth muscle Actin Mouse mAb (Ab088443)

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Mouse Pancreas Tissue
Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

IHC: Alpha smooth muscle Actin Mouse mAb (Ab088443)

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC
DAB staining on IHC
Sample: Mouse Spleen Tissue
Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Application

Application	Dilution info
IHC	5-20 µg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0404290	Certificate of Analysis	Apr 09, 2024	Ab088443
ZJ23F0800857	Certificate of Analysis	Aug 22, 2023	Ab088443
ZJ23F0800856	Certificate of Analysis	Aug 22, 2023	Ab088443

Alternative Products

Documents & Articles

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Categories: Protocols

Tags:

IHC Monoclonal antibodies Immunohistochemistry Frozen section Recombinant Antibodies

Immunohistochemistry (IHC) staining antigen retrieval protocol

Categories: Protocols

Tags:

IHC Monoclonal antibodies Immunohistochemistry Recombinant Antibodies Antigen Retrieval

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