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Pofut1 Antibody - Primary antibody, specific to Pofut1, Rabbit IgG

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Pofut1 Antibody - Primary antibody, specific to Pofut1, Rabbit IgG

Grade & Purity:

High Performance

See COA

Application:

IHC
WB

Host species: Rabbit
Reactive species: Human,Mouse,Rat
Isotype: Rabbit IgG
Conjugation: Unconjugated

In stock

Item Number

Ab122526

Grouped product items
SKU	Size	Availability	Price	Qty
Ab122526-10μl	10μl	10	$79.90
Ab122526-50μl	50μl	4	$219.90
Ab122526-100μl	100μl	2	$349.90
Ab122526-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,999.90

pAb; Rabbit anti Human Pofut1 Antibody; WB, IHC; Unconjugated

View related series

Click to view target information: POFUT1

Accession#: Q9H488 (1) Gene ID: 23509 (1) POFUT1 (1) Protein O-Fucosyltransferase 1 (1)

Basic Description

Product Name	Pofut1 Antibody - Primary antibody, specific to Pofut1, Rabbit IgG
Synonyms	FUT 12 antibody \| FUT12 antibody \| GDP fucose protein O fucosyltransferase 1 antibody \| GDP-fucose protein O-fucosyltransferase 1 antibody \| O Fuc T antibody \| o fucosyltransferase protein antibody \| O FucT 1 antibody \| O FucT antibody \| O FucT1 antibody
Specifications & Purity	Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Host species	Rabbit
Specificity	Pofut1
Immunogen	Recombinant POFUT1 expressed in E.coli (AA 238-388).
Positive Control	WB: Hep G2, HeLa, MOLT-4, NIH/3T3 and C6 cell lysates. IHC: Human kidney, liver, lung cancer, liver cancer tissues.
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description	Rabbit anti Human Pofut1 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 0.01-2 µg/mL IHC: 5-20 µg/mL Protein Function: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified + Protein A purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

POFUT1 Tbio GDP-fucose protein O-fucosyltransferase 1 (0 Activities)

Discover Target: POFUT1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: Pofut1 Antibody (Ab122526)

Pofut1 Antibody (Ab122526) - Western Blot
All lanes: Pofut1 Antibody (Ab122526) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 46 kDa
Exposure time: 9.2 s

IHC: Pofut1 Antibody (Ab122526)

Pofut1 Antibody (Ab122526) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 20 µg/mL Pofut1 Antibody (Ab122526)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Pofut1 Antibody (Ab122526)

Pofut1 Antibody (Ab122526) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 20 µg/mL Pofut1 Antibody (Ab122526)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Pofut1 Antibody (Ab122526)

Pofut1 Antibody (Ab122526) - IHC
DAB staining on IHC
Samples: Human Lung cancer Tissue
Primary Ab: 20 µg/mL Pofut1 Antibody (Ab122526)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Pofut1 Antibody (Ab122526)

Pofut1 Antibody (Ab122526) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 20 µg/mL Pofut1 Antibody (Ab122526)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Pofut1 Antibody (Ab122526)

Pofut1 Antibody (Ab122526) - IHC
DAB staining on IHC
Sample: Human Liver Tissue
Primary Ab: 20 µg/mL Pofut1 Antibody (Ab122526)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Pofut1 Antibody (Ab122526)

Pofut1 Antibody (Ab122526) - IHC
DAB staining on IHC-P
Sample: Human Liver cancer Tissue
Primary Ab: 20 µg/mL Pofut1 Antibody (Ab122526)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: Pofut1 Antibody (Ab122526)

Pofut1 Antibody (Ab122526) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL Pofut1 Antibody (Ab122526)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Application

Application	Dilution info
IHC	5-20 µg/mL
WB	0.01-2 µg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0505299	Certificate of Analysis	May 09, 2024	Ab122526
ZJ24F0505298	Certificate of Analysis	May 09, 2024	Ab122526
ZJ24F0505297	Certificate of Analysis	May 09, 2024	Ab122526

Documents & Articles

Sodium Azide Removal Protocol

Categories: Protocols

Tags:

Recombinant antibody Sodium Azide Removal Antibody Purification Sephadex G-25 Sodium Azide-Free Antibody

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