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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab106592-10μl
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10μl |
10
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$69.90
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Ab106592-50μl
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50μl |
4
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$199.90
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Ab106592-100μl
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100μl |
2
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$289.90
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Ab106592-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$2,599.90
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pAb; Rabbit anti Human GSDMD Antibody; WB; Unconjugated
| Product Name | GSDMD Antibody - Primary antibody, specific to GSDMD, Rabbit IgG |
|---|---|
| Synonyms | Gasdermin domain-containing protein 1 | 1810036L03Rik antibody | DF 5L antibody | DF5L antibody | DFNA 5L antibody | DFNA5L antibody | FKSG 10 antibody | FKSG10 antibody | FLJ12150 antibody | Gasdermin antibody | Gasdermin D antibody | Gasdermin domain co |
| Specifications & Purity | Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA |
| Host species | Rabbit |
| Specificity | GSDMD |
| Immunogen | Recombinant GSDMD expressed in E.coli (AA 232-484) |
| Positive Control | WB: A431, U2OS and NIH/3T3 cell lysates. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Rabbit anti Human GSDMD Antibody, Polyclonal, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified and Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
GSDMD Antibody (Ab106592) - Western Blot
All lanes: GSDMD Antibody (Ab106592) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 32, 45, 51kDa
Exposure time: 30.0 s
GSDMD Antibody (Ab106592) - IF/ICC
IF analysis of GSDMD Antibody (Ab106592) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with GSDMD Antibody (Ab106592) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
| Application | Dilution info |
|---|---|
| WB |
0.01-2 µg/mL
|
| IF/ICC |
2.5 µg/mL
|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | May 11, 2024 | Ab106592 | |
| Certificate of Analysis | May 11, 2024 | Ab106592 | |
| Certificate of Analysis | May 11, 2024 | Ab106592 |