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Goat Anti-Mouse IgG H&L (FITC) - Secondary antibody, Goat IgG

    Application:
  • Flow Cytometry
  • IF/ICC
  • Host species: Goat
  • Reactive species: Mouse
  • Isotype: Goat IgG
  • Conjugation: FITC
In stock
Item Number
Ab179003
Grouped product items
SKU Size
Availability
Price Qty
Ab179003-100μg
100μg
≥10
$29.90
Ab179003-1mg
1mg
1
$119.90

Secondary Antibody; Goat Anti-Mouse IgG H&L (FITC); IF, Flow

Basic Description

Product Name Goat Anti-Mouse IgG H&L (FITC) - Secondary antibody, Goat IgG
Synonyms Goat Anti-Mouse IgG H&L Antibody (FITC) | Anti-Mouse IgG H&L Antibody (FITC) | Mouse IgG H&L Antibody (FITC) | FITC-conjugated Goat Anti-Mouse IgG (H+L) | Goat Anti Mouse IgG Antibody (FITC) | Anti-Mouse IgG Antibody (FITC) | Mouse IgG Antibody (FITC) | G
Specifications & Purity ExactAb™, Validated, Azide Free, Ex:498nm, Em:517nm, 1.0 mg/mL
Host species Goat
Immunogen Mouse IgG
Conjugation FITC
Excitation(nm) Blue Laser(488nm)
Emission(nm) 517nm
Ex/Em(nm) Ex:498nm;Em:517nm
Grade Azide Free, Em:517nm, Ex:498nm, ExactAb™, Validated
Product Description

Application
IF: 1:1,000
Flow: 1:100-1:2,000

Product Properties

Isotype Goat IgG
SDS-PAGE 150 kDa
Purification Method Antigen affinity purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

Goat Anti-Mouse IgG H&L (FITC) (Ab179003) - IF
IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/1600 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Goat Anti-Mouse IgG H&L (FITC) (Ab179003) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with CD45 Mouse mAb (Ab094902). Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/300 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
IF/ICC

1:1000

Flow Cytometry

1:100-1:2000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number Certificate Type Date Item
ZJ24R1201659 Certificate of Analysis Dec 26, 2024 Ab179003
ZJ23F1202024 Certificate of Analysis Dec 13, 2023 Ab179003
ZJ23F1202023 Certificate of Analysis Dec 13, 2023 Ab179003
ZJ23R1200181 Certificate of Analysis Dec 07, 2023 Ab179003

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Citations of This Product

1. Jialin Hu, Lingling Guo, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Xinxin Xu.  (2024)  Rapid determination of α-lactalbumin in raw cow's milk and recombined milk using an immunochromatographic assay strip analysis method.  Food Bioscience,  61  (104936). 

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