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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab185413-10μg
|
10μg |
4
|
$49.90
|
|
|
Ab185413-25μg
|
25μg |
4
|
$99.90
|
|
|
Ab185413-100μg
|
100μg |
1
|
$239.90
|
|
mAb (2D1); Mouse anti Human CD45 Antibody (Biotin); Flow
| Product Name | CD45 Mouse mAb (Biotin) - Primary antibody, specific to CD45, Mouse IgG1 |
|---|---|
| Synonyms | CD45 Monoclonal Antibody (2D1), Biotin | Biotin Anti-Human CD45 Antibody[2D1] | Biotin Mouse Anti-Human CD45 | Biotin anti-human CD45 Antibody | T200 leukocyte common antigen antibody | T200 glycoprotein antibody | T200 antibody | Receptor-type tyrosine-p |
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Host species | Mouse |
| Specificity | CD45 |
| Immunogen | Human PBMC |
| Positive Control | Flow: Jurkat |
| Conjugation | Biotin |
| Grade | ExactAb™, Validated |
| Product Description |
Application: |
| Isotype | Mouse IgG1 |
|---|---|
| Purification Method | Protein G purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
CD45 Mouse mAb (Biotin) (Ab185413) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab185413) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤0.5μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume. |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Apr 25, 2025 | Ab185413 | |
| Certificate of Analysis | Jul 22, 2024 | Ab185413 | |
| Certificate of Analysis | Jul 22, 2024 | Ab185413 | |
| Certificate of Analysis | Jul 12, 2024 | Ab185413 |
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