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CD45 Mouse mAb (Biotin) - Primary antibody, specific to CD45, Mouse IgG1

    Application:
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: HI30
  • Conjugation: Biotin
In stock
Item Number
Ab179532
Grouped product items
SKU Size
Availability
Price Qty
Ab179532-10μg
10μg
7
$19.90
Ab179532-25μg
25μg
2
$29.90
Ab179532-100μg
100μg
1
$75.90

mAb (HI30); Mouse anti Human CD45 Antibody (Biotin); Flow

Basic Description

Product Name CD45 Mouse mAb (Biotin) - Primary antibody, specific to CD45, Mouse IgG1
Synonyms B220 | CD_antigen: CD45 | CD45 antigen | CD45 | CD45R | EC 3.1.3.48 | EC:3.1.3.48 | GP180 | LCA | L-CA | Leukocyte common antigen | LY5 | protein tyrosine phosphatase, receptor type, c polypeptide | PTPRC | receptor-type tyrosine-protein phosphatase C | T
Specifications & Purity ExactAb™, Validated, Azide Free, 0.5 mg/mL
Host species Mouse
Specificity CD45
Immunogen Peripheral blood mononuclear cells
Positive Control Flow: Jurkat
Conjugation Biotin
Grade Azide Free, ExactAb™, Validated
Product Description

Application:

Flow: ≤0.5μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
Purification Method Protein A purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

CD45 Mouse mAb (Biotin) (Ab179532) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab179532) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤0.5μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0404694 Certificate of Analysis Apr 19, 2024 Ab179532
ZJ24F0404693 Certificate of Analysis Apr 19, 2024 Ab179532
ZJ24F0404695 Certificate of Analysis Apr 19, 2024 Ab179532

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