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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab179532-10μg
|
10μg |
7
|
$19.90
|
|
|
Ab179532-25μg
|
25μg |
2
|
$29.90
|
|
|
Ab179532-100μg
|
100μg |
1
|
$75.90
|
|
mAb (HI30); Mouse anti Human CD45 Antibody (Biotin); Flow
| Product Name | CD45 Mouse mAb (Biotin) - Primary antibody, specific to CD45, Mouse IgG1 |
|---|---|
| Synonyms | B220 | CD_antigen: CD45 | CD45 antigen | CD45 | CD45R | EC 3.1.3.48 | EC:3.1.3.48 | GP180 | LCA | L-CA | Leukocyte common antigen | LY5 | protein tyrosine phosphatase, receptor type, c polypeptide | PTPRC | receptor-type tyrosine-protein phosphatase C | T |
| Specifications & Purity | ExactAb™, Validated, Azide Free, 0.5 mg/mL |
| Host species | Mouse |
| Specificity | CD45 |
| Immunogen | Peripheral blood mononuclear cells |
| Positive Control | Flow: Jurkat |
| Conjugation | Biotin |
| Grade | Azide Free, ExactAb™, Validated |
| Product Description |
Application: Flow: ≤0.5μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume or 100 µL of whole blood. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
CD45 Mouse mAb (Biotin) (Ab179532) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab179532) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤0.5μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume. |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Apr 19, 2024 | Ab179532 | |
| Certificate of Analysis | Apr 19, 2024 | Ab179532 | |
| Certificate of Analysis | Apr 19, 2024 | Ab179532 |
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